Isolamento e identificação dos agentes da cromomicose em Belo Horizonte

Oliveira, Luiz Gonzaga de

doi:10.1590/s0037-86821973000100002

Cited by 30 publications

(20 citation statements)

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“…The pathogenic strain of F. pedrosoi (5VPL) used in this study was isolated from a human patient with CBM (Oliveira et al, 1973). Stock cultures were maintained in Sabouraud-dextrose (glucose) agar, at 4 uC.…”

Section: Methodsmentioning

confidence: 99%

Ectophosphatase activity in conidial forms of Fonsecaea pedrosoi is modulated by exogenous phosphate and influences fungal adhesion to mammalian cells

et al. 2004

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Section: Methodsmentioning

confidence: 99%

Ectophosphatase activity in conidial forms of Fonsecaea pedrosoi is modulated by exogenous phosphate and influences fungal adhesion to mammalian cells

et al. 2004

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“…A pathogenic strain of F. pedrosoi was isolated from a human patient with chromoblastomycosis (Oliveira et al, 1973). The fungus was maintained in Sabouraud dextrose agar (SDA), covered with a layer of mineral oil (0.5 ml), at 4 "C. Transfers were made at six month intervals.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Fonsecaea pedrosoi melanin

Alviano¹,

Farbiarz

Souza

et al. 1991

Journal of General Microbiology

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The constituents of the melanin complex from mycelial forms of Fonsecaea pedrosoi were partially characterized. The pigment was mainly accumulated on large alkali-extractable, electron-dense cytoplasmic bodies (melanosomes) and, apparently, on the outer layer of the cell wall as external deposits within verrucose outgrowths. Using electron microscopy and Thiky's periodate/thiosemicarbazide/silver proteinate staining method, glycogenlike particles were also detected at the periphery of the cells. Melanin constituents comprised aromatic and aliphatic/glycosidic structures with a predominance of the latter. Infrared spectra showed the presence of hydroxyl, carbonyl and carboxyl groups. The aliphatic/glycosidic moiety consisted of fatty acids and polysaccharides with protein, in a ratio protein/polysaccharide 1 : 15. Rhamnose, mannose, galactose and glucose (in the ratio 1 : 2: 4: 3.5) were the constituents of the polysaccharide. Lipid components included even-numbered, saturated and unsaturated fatty acids (in the ratio 2: 1) ranging from C16 to Cis. Palmitic and oleic acids were the prominent fatty acids. Aspartic and glutamic acids, leucine, glycine and alanine were the major amino acids. Nonpigmented cells of F.pedrosoi were studied for comparison with the pigmented forms: they did not accumulate acidinsoluble precursors of melanin.

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“…A human isolate of F. pedrosoi (strain 5VLP, Oliveira et al, 1973) was used in the present work. Stock cultures were maintained on Saubouraud's glucose agar medium under mineral oil and kept at 4°C.…”

Section: Microorganism and Growth Conditionsmentioning

confidence: 99%