Ectophosphatase activity in conidial forms of Fonsecaea pedrosoi is modulated by exogenous phosphate and influences fungal adhesion to mammalian cells

Kneipp, Lucimar F.; Rodrigues, Márcio L.; Holandino, Carla; Esteves, Fabiano F.; Souto-Padrón, Thaïs; Alviano, Celuta Sales; Travassos, Luiz R.; Meyer‐Fernandes, José Roberto

doi:10.1099/mic.0.27405-0

Cited by 59 publications

(79 citation statements)

References 38 publications

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“…The walls of F. pedrosoi muriform cells present higher phosphatase activity than do conidia or hyphae (207), and phosphatase activity enhances host cell adhesion in both F. pedrosoi (206) and Rhinocladiella aquaspersa (208). The cytolytic effect of ATP suggests that ecto-ATPases found at the surface of F. pedrosoi may favor fungus survival in hostile environments such as the human body (209,210).…”

Section: Extracellular Enzymes and Metabolitesmentioning

confidence: 99%

“…Proteolytic enzymes can be detrimental to various components of the host defense mechanisms, leading to immunological escape or antimicrobial resistance (204,205). Kneipp et al demonstrated that muriform cells, in comparison to conidia and mycelia, have high phosphatase activity associated with pathogenicity (206,207). Phosphorylated substrates were hydrolyzed by surface ectophosphatases.…”

Section: Extracellular Enzymes and Metabolitesmentioning

confidence: 99%

“…Chronicity developed after failure of costimulation of pattern recognition receptors (PRRs) (206). In cases of Fonsecaea pedrosoi, initial fungal recognition is mediated only by C-type lectin receptors (CLRs), leading to the flawed stimulation of proinflammatory compounds.…”

Section: Innate Immune Responsementioning

confidence: 99%

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Chromoblastomycosis

et al. 2017

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SUMMARY Chromoblastomycosis (CBM), also known as chromomycosis, is one of the most prevalent implantation fungal infections, being the most common of the gamut of mycoses caused by melanized or brown-pigmented fungi. CBM is mainly a tropical or subtropical disease that may affect individuals with certain risk factors around the world. The following characteristics are associated with this disease: (i) traumatic inoculation by implantation from an environmental source, leading to an initial cutaneous lesion at the inoculation site; (ii) chronic and progressive cutaneous and subcutaneous tissular involvement associated with fibrotic and granulomatous reactions associated with microabscesses and often with tissue proliferation; (iii) a nonprotective T helper type 2 (Th2) immune response with ineffective humoral involvement; and (iv) the presence of muriform (sclerotic) cells embedded in the affected tissue. CBM lesions are clinically polymorphic and are commonly misdiagnosed as various other infectious and noninfectious diseases. In its more severe clinical forms, CBM may cause an incapacity for labor due to fibrotic sequelae and also due to a series of clinical complications, and if not recognized at an early stage, this disease can be refractory to antifungal therapy.

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Section: Extracellular Enzymes and Metabolitesmentioning

confidence: 99%

Section: Extracellular Enzymes and Metabolitesmentioning

confidence: 99%

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Chromoblastomycosis

et al. 2017

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“…Several studies have demonstrated that ecto-phosphatase activities are involved in cell adhesion and invasion of distinct microorganisms, including bacteria (Ivanov et al 2005 ), fungal cells (Kneipp et al 2004 ;Collopy-Junior et al 2006 ;Kiffer-Moreira et al 2007 ;Portela et al 2010 ;Kneipp et al 2012 ;CosentinoGomes et al 2013 ) and protozoa Aguirre-García et al 2002 ;Anaya-Ruiz et al 2003 ;Dos Santos et al 2012 ). Protein tyrosine phosphatases are increasingly recognized as important effectors of host-pathogen interactions (Heneberg 2012 ).…”

Section: Ecto-phosphatases As Virulence Markers: Possible Roles In Admentioning

confidence: 98%

Ecto-nucleotidases and Ecto-phosphatases from Leishmania and Trypanosoma Parasites

Freitas-Mesquita

Meyer‐Fernandes

2013

Subcellular Biochemistry

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Ecto-enzymes can be defined as membrane-bound proteins that have their active site facing the extracellular millieu. In trypanosomatids, the physiological roles of these enzymes remain to be completed elucidated; however, many important events have already been related to them, such as the survival of parasites during their complex life cycle and the successful establishment of host infection. This chapter focuses on two remarkable classes of ecto-enzymes: ecto-nucleotidases and ecto-phosphatases, summarizing their occurrence and possible physiological roles in Leishmania and Trypanosoma genera. Ecto-nucleotidases are characterized by their ability to hydrolyze extracellular nucleotides, playing an important role in purinergic signaling. By the action of these ecto-enzymes, parasites are capable of modulating the host immune system, which leads to a successful parasite infection. Furthermore, ecto-nucleotidases are also involved in the purine salvage pathway, acting in the generation of nucleosides that are able to cross plasma membrane via specialized transporters. Another important ecto-enzyme present in a vast number of pathogenic organisms is the ecto-phosphatase. These enzymes are able to hydrolyze extracellular phosphorylated substrates, releasing free inorganic phosphate that can be internalized by the cell, crossing the plasma membrane through a Pi-transporter. Ecto-phosphatases are also involved in the invasion and survival of parasite in the host cells. Several alternative functions have been suggested for these enzymes in parasites, such as participation in their proliferation, differentiation, nutrition and protection. In this context, the present chapter provides an overview of recent discoveries related to the occurrence of ecto-nucleotidase and ecto-phosphatase activities in Leishmania and Trypanosoma parasites.

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“…A functional Ecm33p is required for cell integrity and for an adequate formation of hyphae, and it is also critical for virulence of this pathogenic yeast. MeyerFernandes and coworkers have studied a phosphatase associated with the cell wall of the chromoblastomycosis agent Fonsecaea pedrosi (Kneipp et al, 2004). They conclude that the presence of this enzyme in the cell wall is relevant for adhesion to mammalian cells.…”

Section: The Cell Wall Determines Interaction With the Environmentmentioning

confidence: 99%

Fungal cell wall biogenesis: building a dynamic interface with the environment

Durán

2004

Microbiology

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Ectophosphatase activity in conidial forms of Fonsecaea pedrosoi is modulated by exogenous phosphate and influences fungal adhesion to mammalian cells

Cited by 59 publications

References 38 publications

Chromoblastomycosis

Chromoblastomycosis

Ecto-nucleotidases and Ecto-phosphatases from Leishmania and Trypanosoma Parasites

Fungal cell wall biogenesis: building a dynamic interface with the environment

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