Isoforms of Amino Acid Transporters in Placental Syncytiotrophoblast: Plasma Membrane Localization and Potential Role in Maternal/Fetal Transport

Cariappa, Rohit; Heath-Monnig, E.; Smith, Carl H.

doi:10.1016/s0143-4004(03)00085-7

Cited by 49 publications

(25 citation statements)

References 178 publications

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“…At least 15 different active amino acid transport systems have been identified in the human placenta, 7 of which involving neutral amino acids. With the advances in molecular biology studies, at least 25 clones of complementary DNA for amino acid or amino acid subunit carrier proteins have been identified in trophoblastic cells (3,4).…”

Section: Introductionmentioning

confidence: 99%

Plasma amino acids in pregnancy, placental intervillous space and preterm newborn infants

Camelo

Martinez

Gonçalves

et al. 2007

Braz J Med Biol Res

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Plasma amino acid levels have never been studied in the placental intervillous space of preterm gestations. Our objective was to determine the possible relationship between plasma amino acids of maternal venous blood (M), of the placental intervillous space (PIVS) and of the umbilical vein (UV) of preterm newborn infants. Plasma amino acid levels were analyzed by ion-exchange chromatography in M from 14 parturients and in the PIVS and UV of their preterm newborn infants. Mean gestational age was 34 ± 2 weeks, weight = 1827 ± 510 g, and all newborns were considered adequate for gestational age. The mean Apgar score was 8 and 9 at the first and fifth minutes. Plasma amino acid values were significantly lower in M than in PIVS (166%), except for aminobutyric acid. On average, plasma amino acid levels were significantly higher in UV than in M (107%) and were closer to PIVS than to M values, except for cystine and aminobutyric acid (P < 0.05). Comparison of the mean plasma amino acid concentrations in the UV of preterm to those of term newborn infants previously studied by our group showed no significant difference, except for proline (P < 0.05), preterm > term. These data suggest that the mechanisms of active amino acid transport are centralized in the syncytiotrophoblast, with their passage to the fetus being an active bidirectional process with asymmetric efflux. PIVS could be a reserve amino acid space for the protection of the fetal compartment from inadequate maternal amino acid variations. Correspondence

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Section: Introductionmentioning

confidence: 99%

Plasma amino acids in pregnancy, placental intervillous space and preterm newborn infants

Camelo

Martinez

Gonçalves

et al. 2007

Braz J Med Biol Res

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“…SNAT3 has been reported to be expressed in the liver, brain and kidney. Moreover, its expression in the placenta has been discussed for a long time [18,22]. SNAT3 mediates glutamine transport with sodium and proton coupled manner, and it contributes to ammonia metabolism in the liver and brain [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Expression and Role of SNAT3 in the Placenta

et al. 2009

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The number of characters in the abstract; 1079 characters without spaces 2 The number of characters in the entire manuscripts; 28922 characters without spaces The number of illustrations; 8The number of references; 40 3 AbstractGlutamine is the most versatile amino acid and its plasma concentration is the highest of all amino acid. Many transporters are therefore involved in glutamine uptake or efflux. Glutamine is actively released from the placenta into fetal circulation. In this study, we examined the alteration of transporters that transport glutamine into fetal circulation as gestation progresses. High expression levels of system A and y + L were found in the rat placenta in the late period of pregnancy and the expression levels of these transporters increased as gestation progressed (p<0.05). On the other hand, the expression of SNAT3, the system N transporter, was detected in the early period of pregnancy and its expression level decreased as gestation progressed (p<0.05). SNAT3 was also found to be expressed in isolated human primary cytotrophoblast cells and its expression level was decreased by their differentiation into syncytiotrophoblast cells (p<0.05). Since this regulation is closely related to glutamine synthetase expression, SNAT3 may play a key role in providing glutamine corresponding to glutamine synthetase function in the early period of gestation. This is the first report on the expression of SNAT3 in the placenta in the early stage of pregnancy.

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“…Despite the fact that the cell line is malignant in origin, it has been demonstrated that the BeWo cell model has many similarities to normal trophoblast cells, which are of relevance to both toxicity and transport assessment. These similarities include placental hormone secretion (Sastry, 1996), close cell apposition and apical microvillous projections (Bode et al, 2006), and the ability to form a confluent monolayer with functional polarity (Bode et al, 2006;Cariappa et al, 2003). BeWo cells have also been shown to differentially express enzymes and transporters such as cytochrome P450 and Pgp with responses similar to trophoblasts in vivo (Jin & Audus, 2005;Thadani et al, 2004;Vähäkangas & Myllynen, 2006).…”

mentioning

confidence: 99%

In vitromodels of the human placental barrier –In regione caecorum rex est luscus

et al. 2015

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Isoforms of Amino Acid Transporters in Placental Syncytiotrophoblast: Plasma Membrane Localization and Potential Role in Maternal/Fetal Transport

Cited by 49 publications

References 178 publications

Plasma amino acids in pregnancy, placental intervillous space and preterm newborn infants

Plasma amino acids in pregnancy, placental intervillous space and preterm newborn infants

Expression and Role of SNAT3 in the Placenta

In vitromodels of the human placental barrier –In regione caecorum rex est luscus

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