<i>In vitro</i>models of the human placental barrier –<i>In regione caecorum rex est luscus</i>

Carreira, Sara Correia; Walker, Laura; Paul, Kai B.; Saunders, Margaret

doi:10.3109/17435390.2013.869628

Cited by 7 publications

(3 citation statements)

References 16 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…30%) and provide limited mechanistic insights [ 9 , 26 ]. Consequently, in vitro transfer models based on microporous supports are frequently used as a complementary approach with increased throughput capability and the possibility to address permeability across individual cell layers [ 25 , 33 – 35 ]. Here, we applied our recently developed human placental co-culture model to investigate translocation across the trophoblast layer (BeWo cells), endothelial layer (HPEC) or the co-culture [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Gold nanoparticle distribution in advanced in vitro and ex vivo human placental barrier models

et al. 2018

View full text Add to dashboard Cite

BackgroundGold nanoparticles (AuNPs) are promising candidates to design the next generation NP-based drug formulations specifically treating maternal, fetal or placental complications with reduced side effects. Profound knowledge on AuNP distribution and effects at the human placental barrier in dependence on the particle properties and surface modifications, however, is currently lacking. Moreover, the predictive value of human placental transfer models for NP translocation studies is not yet clearly understood, in particular with regards to differences between static and dynamic exposures. To understand if small (3–4 nm) AuNPs with different surface modifications (PEGylated versus carboxylated) are taken up and cross the human placental barrier, we performed translocation studies in a static human in vitro co-culture placenta model and the dynamic human ex vivo placental perfusion model. The samples were analysed using ICP-MS, laser ablation-ICP-MS and TEM analysis for sensitive, label-free detection of AuNPs.ResultsAfter 24 h of exposure, both AuNP types crossed the human placental barrier in vitro, although in low amounts. Even though cellular uptake was higher for carboxylated AuNPs, translocation was slightly increased for PEGylated AuNPs. After 6 h of perfusion, only PEGylated AuNPs were observed in the fetal circulation and tissue accumulation was similar for both AuNP types. While PEGylated AuNPs were highly stable in the biological media and provided consistent results among the two placenta models, carboxylated AuNPs agglomerated and adhered to the perfusion device, resulting in different cellular doses under static and dynamic exposure conditions.ConclusionsGold nanoparticles cross the human placental barrier in limited amounts and accumulate in placental tissue, depending on their size- and/or surface modification. However, it is challenging to identify the contribution of individual characteristics since they often affect colloidal particle stability, resulting in different biological interaction in particular under static versus dynamic conditions. This study highlights that human ex vivo and in vitro placenta models can provide valuable mechanistic insights on NP uptake and translocation if accounting for NP stability and non-specific interactions with the test system.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0406-6) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Gold nanoparticle distribution in advanced in vitro and ex vivo human placental barrier models

et al. 2018

View full text Add to dashboard Cite

show abstract

“… 2015 ) and the limitations of the approaches have been discussed (Correia Carreira et al. 2015b ; Keelan et al. 2015 ; Nikitina, Dohr, and Juch 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Dendritic polyglycerol nanoparticles show charge dependent bio-distribution in early human placental explants and reduce hCG secretion

et al. 2018

View full text Add to dashboard Cite

A thorough understanding of nanoparticle bio-distribution at the feto-maternal interface will be a prerequisite for their diagnostic or therapeutic application in women of childbearing age and for teratologic risk assessment. Therefore, the tissue interaction of biocompatible dendritic polyglycerol nanoparticles (dPG-NPs) with first- trimester human placental explants were analyzed and compared to less sophisticated trophoblast-cell based models. First-trimester human placental explants, BeWo cells and primary trophoblast cells from human term placenta were exposed to fluorescence labeled, ∼5 nm dPG-NPs, with differently charged surfaces, at concentrations of 1 µM and 10 nM, for 6 and 24 h. Accumulation of dPGs was visualized by fluorescence microscopy. To assess the impact of dPG-NP on trophoblast integrity and endocrine function, LDH, and hCG releases were measured. A dose- and charge-dependent accumulation of dPG-NPs was observed at the early placental barrier and in cell lines, with positive dPG-NP-surface causing deposits even in the mesenchymal core of the placental villi. No signs of plasma membrane damage could be detected. After 24 h we observed a significant reduction of hCG secretion in placental explants, without significant changes in trophoblast apoptosis, at low concentrations of charged dPG-NPs. In conclusion, dPG-NP’s surface charge substantially influences their bio-distribution at the feto-maternal interface, with positive charge facilitating trans-trophoblast passage, and in contrast to more artificial models, the first-trimester placental explant culture model reveals potentially hazardous influences of charged dPG-NPs on early placental physiology.

show abstract

“…Among these different cell lines, the BeWo cells and its b30 subclone are the most commonly used cell lines to study placental transport. Culturing the BeWo b30 cells on permeable membranes in transwells where a continuous cell layer is formed results in a model where the maternal and foetal side are represented by respectively the apical and basolateral compartments of the transwells (Correia Carreira et al 2015b;Correia Carreira et al 2015a;Liu et al 1997). This model has been used to study the transport of different NPs e.g.…”

Section: )mentioning

confidence: 99%

In vitro testing strategies for hazard assessment of nanoparticles

Abdelkhaliq¹

View full text Add to dashboard Cite

A549 lung cells exposed to SiO2 NPs in serum-free medium (to prevent corona formation) had higher cellular internalization, association and cytotoxicity compared to exposure to the SiO2 NPs under serum-rich conditions (Lesniak et al. 2012). Not only the presence of the protein corona has an effect on the biological interactions and effects of the NPs, but also the composition of the corona affects these characteristics (Tedja et al. 2012). Recently, it has been shown that SiO2 NPs with a different protein corona can be recognized by different cell receptors, resulting in different mechanisms of cellular uptake (Francia et al. 2019). The main protein candidates in the protein corona would be mainly lung surfactant proteins, serum proteins, or enzymes and food biomolecules depending on whether the exposure took place via the inhalation, intravenous, or oral route, respectively (Riediker et al. 2019). Dissolution The potential of part of the NPs, especially metal-based NPs, to dissolve is another property of the NPs that defines their biological interactions and influences their biopersistence and possible accumulation (Borm et al. 2006). Dissolution and ion release could take place before, during or after the contact of NPs with biological media, e.g. in stock suspensions containing NPs or in the cell culture medium used in in vitro cell models (Maurer et al. 2014; Borm et al. 2006). The dissolution rate of NPs differs between the NPs, as the dissolution of AuNPs was limited (0.6 -3) (Carlander et al. 2019) while AgNPs were found to dissolve up to 90% (Kittler et al. 2010) and ZnO NPs (Eixenberger et al. 2017) and CuO NPs (Liu et al. 2020) can dissolve completely. Dissolution of NPs is a dynamic process that is influenced by intrinsic properties of the NPs like the size, surface chemistry or chemical composition and by properties of the surrounding environment like the temperature, pH, ionic strength, light and presence of organic components in the suspensions (Misra et al. 2012). The size of the NPs is an important factor influencing the NP dissolution, as it was found that small AgNPs with a size of 10 nm dissolved and released relatively more silver ions (Ag + ) than the larger AgNPs (40, 50 and 75 nm) (Gliga et al. 2014). Furthermore, the study of Studer et al. demonstrated that the surface chemistry of the NPs also affects the dissolution of the NPs where coating of metal oxide NPs

show abstract

In vitromodels of the human placental barrier –In regione caecorum rex est luscus

Cited by 7 publications

References 16 publications

Gold nanoparticle distribution in advanced in vitro and ex vivo human placental barrier models

Gold nanoparticle distribution in advanced in vitro and ex vivo human placental barrier models

Dendritic polyglycerol nanoparticles show charge dependent bio-distribution in early human placental explants and reduce hCG secretion

In vitro testing strategies for hazard assessment of nanoparticles

Contact Info

Product

Resources

About