Isoenzyme patterns as a tool in taxonomy of chlorococcal algae

Kilias, Harald

doi:10.1002/elps.1150090926

Cited by 3 publications

(1 citation statement)

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“…The prerequisites were sterile cultured isolates maintained under defined conditions and cultures of type species for comparison (Tschermak-Woess 1988). Later, the photobiont diversity within thalli of conspecific lichen-forming fungi were biochemically analysed, the focus being on isoenzyme patterns (Kilias 1988; Kilias et al 1988; Fahselt 1989). With molecular techniques and algal-specific primers applied to whole lichen DNA, the photobionts can be conveniently identified at the species level without any isolation and culturing (Beck et al 1998; Friedl et al 2000; Dahlkild et al 2001; Helms et al 2001; Beck et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of sterile culturedTrebouxiaphotobionts associated with the lichen-forming fungusXanthoria parietinavisualized with RAPD-PCR fingerprinting techniques

Nyati

Werth

Honegger³

2013

The Lichenologist

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Photobiont diversity within populations of Xanthoria parietina was studied at the species level by means of ITS analyses and at the subspecific level with fingerprinting techniques (RAPD-PCR) applied to sterile cultured algal isolates. Populations from coastal, rural and urban sites from NW, SW and central France and from NE Switzerland were investigated. Between 8 and 63 samples per population, altogether 150 isolates, were subjected to phenetic and ordination analyses. Epiphytic samples of X. parietina associated with different genotypes of Trebouxia decolorans but saxicolous samples contained T. arboricola. For comparison the T. gelatinosa photobiont of a small population of Teloschistes chrysophthalmus (4 samples) was investigated. ITS sequences of T. decolorans isolates from different geographic locations were largely similar. In all populations a surprisingly high diversity of genotypes was observed in Trebouxia isolated from lichen thalli growing side by side. As Trebouxia spp. are assumed to be asexually reproducing haplonts, the genetic background of this diversity is discussed. Fingerprinting techniques are a powerful tool for obtaining valuable insights into the genetic diversity within the algal partner of lichen-forming fungi at the population level, provided that sterile cultured isolates are available.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity of sterile culturedTrebouxiaphotobionts associated with the lichen-forming fungusXanthoria parietinavisualized with RAPD-PCR fingerprinting techniques

Nyati

Werth

Honegger³

2013

The Lichenologist

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Classification of wheat varieties by isoelectric focusing patterns of gliadins and neural network

1994

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Classification of wheat varieties, using isoelectric focusing patterns of the gliadins, image processing and neural networks, is described. The method was compared to a statistical classification method, discriminant analysis. The isoelectric point and the area of each band were calculated by image processing. Different methods of presenting the electrophoretic patterns to the neural network were studied. The most effective method was transformation of the electrophoretic pattern to a small (11 x 47 pixels) representation of the original digitized image, which was presented to the neural network as a vector. The neural network was trained with a number of patterns and tested with new patterns from different electrophoretic runs of the same wheat varieties. In this study we used ten different wheat varieties and the neural network was able to classify 95.5% of the patterns correctly. The statistical classification method classified the same data set 91.8% correctly. We conclude that both the neural network and discriminant analysis were able to classify the patterns correctly with a high degree of certainty. The patterns that were misclassified were indistinguishable by visual inspection.

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Phytogeography of Southern Hemisphere Lichens

Galloway

1991

Quantitative Approaches to Phytogeography

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Isoenzyme patterns as a tool in taxonomy of chlorococcal algae

Cited by 3 publications

References 21 publications

Genetic diversity of sterile culturedTrebouxiaphotobionts associated with the lichen-forming fungusXanthoria parietinavisualized with RAPD-PCR fingerprinting techniques

Genetic diversity of sterile culturedTrebouxiaphotobionts associated with the lichen-forming fungusXanthoria parietinavisualized with RAPD-PCR fingerprinting techniques

Classification of wheat varieties by isoelectric focusing patterns of gliadins and neural network

Phytogeography of Southern Hemisphere Lichens

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