1984
DOI: 10.1002/ijc.2910340111
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Isoenzyme pattern and subcellular localization of hexokinases in human breast cancer and nonpathological breast tissue

Abstract: Subcellular distribution of hexokinase (HK) isoenzymes in 22 human breast cancers (21 primary cancers and 1 axillary metastatic growth) and 7 non-pathological human mammary gland tissue samples was studied with starch gel electrophoresis on isolated cell fractions obtained by differential centrifugation. Fractions used were cytosol, mitochondria and microsomes. A comparison of two methods for detecting HK activity was made, yielding different results regarding HK II and HK III. A method based on the ability of… Show more

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Cited by 21 publications
(13 citation statements)
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“…In keeping with its activity to inhibit the PTP by protein-protein interactions, HKII is found upregulated in tumors (Shinohara et al, 1991;Rempel et al, 1996;AzoulayZohar et al, 2004). One study detected this enzyme in 45% of all breast tumor samples investigated (Gudnason et al, 1984). Besides the activation of the promotor of the HKII gene, an amplification of the gene has also been observed (Rempel et al, 1996).…”
Section: Subunits Of the Permeability Transition Pore Are Differentiamentioning
confidence: 99%
“…In keeping with its activity to inhibit the PTP by protein-protein interactions, HKII is found upregulated in tumors (Shinohara et al, 1991;Rempel et al, 1996;AzoulayZohar et al, 2004). One study detected this enzyme in 45% of all breast tumor samples investigated (Gudnason et al, 1984). Besides the activation of the promotor of the HKII gene, an amplification of the gene has also been observed (Rempel et al, 1996).…”
Section: Subunits Of the Permeability Transition Pore Are Differentiamentioning
confidence: 99%
“…Non-pathological samples were treated in the same way as tumor samples. No differences in the hexokinase isoenzyme patterns were observed between freshly prepared samples and previously frozen ones [11].…”
Section: Tissue Samplesmentioning
confidence: 69%
“…Postmitochondrial and cytosolic fractions (5-10 mg/ml) were lyophilized to dryness for concentration and stored desiccated at -70 ° C prior to electrophoresis. No difference in hexokinase isozyme patterns was detected before and after lyophilization [11].…”
Section: Preparation Of Cytosolic Fractionsmentioning
confidence: 94%
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“…De hecho, la Hx-2, se expresa en niveles significativamente altos en los gliomas (Muzi et al, 2001). Este mismo patrón de comportamiento se observa en otras células tumorales, donde el 45% de todas las muestras examinadas presentaron altos niveles de esta isoforma (Gudnason et al, 1984). En este sentido, aunque la Hx-1 es la isoforma predominante en la retina, los retinoblastomas expresan cantidades considerables de la Hx-1 (Beemer et al, 1982).…”
Section: Fosforilación De La Glucosa Y Mecanismos De Regulaciónunclassified