Isoenzyme characterization of Leishmania braziliensis braziliensis isolates obtained from Bolivian and Peruvian patients

Revollo, Susana; Dimier-David, L.; David, C.; Lyevre, P.; Camacho, Clara; Dedet, Jean-Pierre

doi:10.1016/0035-9203(92)90231-z

Cited by 16 publications

(6 citation statements)

References 11 publications

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“…( V .) braziliensis is polymorphic [5, 10, 35, 36, 53]. The lack of polymorphism in our study is perhaps due to the fact that only three species of phlebotomine sand flies have been incriminated as the vectors of L .…”

Section: Discussionmentioning

confidence: 72%

Phenotypic characterization ofLeishmaniaspp. causing cutaneous leishmaniasis in the lower Amazon region, western Pará state, Brazil, reveals a putative hybrid parasite,Leishmania(Viannia)guyanensis × Leishmania(Viannia)shawi shawi

et al. 2014

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We phenotypically characterized 43 leishmanial parasites from cutaneous leishmaniasis by isoenzyme electrophoresis and the indirect immunofluorescence antibody test (23 McAbs). Identifications revealed 11 (25.6%) strains of Leishmania (V.) braziliensis, 4 (9.3%) of L. (V.) shawi shawi, 7 (16.3%) of L. (V.) shawi santarensis, 6 (13.9%) of L. (V.) guyanensis and L. (V.) lainsoni, 2 (4.7%) of L. (L.) amazonensis, and 7 (16.3%) of a putative hybrid parasite, L. (V.) guyanensis/L. (V.) shawi shawi. McAbs detected three different serodemes of L. (V.) braziliensis: I-7, II-1, and III-3 strains. Among the strains of L. (V.) shawi we identified two populations: one (7 strains) expressing the B19 epitope that was previously considered to be species-specific for L. (V.) guyanensis. We have given this population sub-specific rank, naming it L. (V.) s. santarensis. The other one (4 strains) did not express the B19 epitope like the L. (V.) shawi reference strain, which we now designate as L. (V.) s. shawi. For the first time in the eastern Brazilian Amazon we register a putative hybrid parasite (7 strains), L. (V.) guyanensis/L. (V.) s. shawi, characterized by a new 6PGDH three-band profile at the level of L. (V.) guyanensis. Its PGM profile, however, was very similar to that of L. (V.) s. shawi. These results suggest that the lower Amazon region – western Pará state, Brazil, represents a biome where L. (V.) guyanensis and L. (V.) s. shawi exchange genetic information.

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Section: Discussionmentioning

confidence: 72%

Phenotypic characterization ofLeishmaniaspp. causing cutaneous leishmaniasis in the lower Amazon region, western Pará state, Brazil, reveals a putative hybrid parasite,Leishmania(Viannia)guyanensis × Leishmania(Viannia)shawi shawi

et al. 2014

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“…The diversity of the geographical origin of the isolates showed that 2 zymodemes PAR5 and PAR6 are present in different counties in the Northwest region of Argentina. The zymodemes that present slight variation in NP2 enzyme (PAR7) have also been reported in Bolivia (Revollo et al 1992) and Colombia (Saravia et al 1985). The population studied (16 isolates) showed low intraspecific variation.…”

Section: Discussionmentioning

confidence: 80%

Characterization of human infection by Leishmania spp. in the Northwest of Argentina: immune response, double infection with Trypanosoma cruzi and species of Leishmania involved

et al. 2003

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The aims of this study were to characterize human American tegumentary leishmaniasis, which includes cutaneous, mucocutaneous and mucosal leishmaniasis, in Northwest Argentina, to determine the prevalence of double infection with Trypanosoma cruzi and to identify the species of Leishmania in this area. Most of the 330 leishmaniasis patients presented cutaneous ulcers (96.1%), 2.4% mucocutaneous and 1.5% the mucosal form ('espundia'). The aetiological agents, determined by isoenzyme electrophoresis, were identified as Leishmania (Viannia) braziliensis in 16 out of 20 isolates and in the remaining 4 as Leishmania (Leishimania) amazonensis, the first ever-documented in Argentina. Sera analysed by ELISA and IFA using complex antigen from both T. cruzi and L. braziliensis showed a very high percentage of positives (66.3-78.2%). When antigens for specific diagnosis of Chagas' disease were used, 40.9% of the leishmaniasis patients were also found to be infected by T. cruzi. These results indicate that the strong immune response against T. cruzi gave no protection to Leishmania, in spite of the serological cross-reaction between these parasites. In addition, we showed that more than 40% of the patients would be misdiagnosed as chagasic if complex antigens, as epimastigotes or soluble fraction from epimastigotes, were used in IFA or ELISA. This is of paramount importance not only because patients' treatment would be associated to misdiagnosis but the fact that in many countries in Central and South America, a positive test for Chagas' disease means a rejection for those seeking employment.

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“…The second is the migrant group, which consists of highland Indians (Quechua and Aymara), comprising -50,000 persons who, after clearing initially forested land, have engaged in agriculture. In addition to being suitable because of the presence of two distinct ethnic populations, this area has been chosen because the prevalence of tegumentary leishmaniasis is supposed to be the highest in the country (David et al 1993) and because the infecting parasite, L. braziliensis, is unique to it (Revello et al 1992).…”

Section: Study Areamentioning

confidence: 99%

Evidence for a Major Gene Controlling Susceptibility to Tegumentary Leishmaniasis in a Recently Exposed Bolivian Population

Alcaïs¹,

Abel²,

David³

et al. 1997

The American Journal of Human Genetics

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Tegumentary leishmaniasis due to Leishmania braziliensis is a parasitic disease that occurs in two stages after the infected sandfly bite: (1) a primary cutaneous lesion followed by (2) a secondary mucosal involvement generally resulting in severe facial deformities. In order to investigate the genetic and environmental factors involved in the development of the cutaneous lesion, a familial study was performed in a region of Bolivia in which the disease is endemic. Complete selection of 118 nuclear families (703 subjects, with 241 patients), each with at least one cutaneous affected subject, was achieved; 41 families were of native origin, and 77 (herein designated "migrant") recently had settled in the area. For the analysis, the trait under study was the time to onset of the primary cutaneous lesion. The start of the follow-up was birth, for native population, or date of arrival in the endemic area, for migrant population. Segregation analysis was performed by use of a model based on survival analysis methods that allows joint estimation of genetic and environmental effects and accounts for gene x covariate interactions. A significant effect of gender, home-forest distance, and forest-related activity was found. In the 77 migrant families there was evidence for a recessive major gene controlling the onset of the primary cutaneous lesion, with residual familial dependences and age x genotype interaction. Penetrance estimations show that young subjects are genetically more susceptible than older subjects, suggesting that this genetic component could concern mechanisms involved in the development of individual protection during childhood. There was also a significant genetic heterogeneity of the sample according to the native/migrant origin of the families, and no major-gene effect was found in the native subsample.

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Isoenzyme characterization of Leishmania braziliensis braziliensis isolates obtained from Bolivian and Peruvian patients

Cited by 16 publications

References 11 publications

Phenotypic characterization ofLeishmaniaspp. causing cutaneous leishmaniasis in the lower Amazon region, western Pará state, Brazil, reveals a putative hybrid parasite,Leishmania(Viannia)guyanensis × Leishmania(Viannia)shawi shawi

Phenotypic characterization ofLeishmaniaspp. causing cutaneous leishmaniasis in the lower Amazon region, western Pará state, Brazil, reveals a putative hybrid parasite,Leishmania(Viannia)guyanensis × Leishmania(Viannia)shawi shawi

Characterization of human infection by Leishmania spp. in the Northwest of Argentina: immune response, double infection with Trypanosoma cruzi and species of Leishmania involved

Evidence for a Major Gene Controlling Susceptibility to Tegumentary Leishmaniasis in a Recently Exposed Bolivian Population

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