Isoenzyme and cotyledon protein variation for identification of black beans (Phaseolus vulgaris L.) with similar seed morphology

Abstract

Methods developed to identify genetically diverse varieties of beans (Phaseolus vulgaris L.) were applied to closely related lines that were difficult to distinguish on the basis of seed morphology. Seedling tissues and seeds of black beans, were examined electrophoretically for isoenzyme and cotyledon protein patterns. Seven enzymes, extracted from seeds or from seedling stem, root or leaf tissues, were compared for polymorphism. Peptidase, polyphenol oxidase, phosphoglucoisomerase and glutamate oxaloacetate transaminase patterns were the same for all lines. Some differences were observed for acid phosphatase, peroxidase and esterase patterns, but complete discrimination of the six selected lines was not possible on the basis of isoenzyme patterns alone. Using polyacrylamide gel electrophoresis (PAGE) of dissociated 0.

Genetic Assessment of Traits and Genetic Relationship in Blackgram (Vigna mungo) Revealed by Isoenzymes

“…Mittal et al (1993) studied eight Vigna radiata cultivars for enzyme activities and concluded that there was a positive correlation of acid phosphatase with 100 seed weight but a negative correlation with number of pods per plant and seed yield. Seven isoenzymes, extracted from seed, seedling, stem, root, or leaf tissues of six closely related lines of black bean, were compared for polymorphism (Driedger et al 1994), and variation was observed for acid phosphatase, peroxidase, and esterase, with some lines possessing unique banding patterns. In this study, bands were identified for trait-specific expression using different isoenzymes that can discriminate among plant accessions.…”