Isoelectric Fractionation, Analysis, and Characterization of Ampholytes in Natural pH Gradients. II. Buffering Capacity and Conductance of Isoionic Ampholytes. A Correction.

Rilbe, Harry; Haugsten, Kjell; Rasmussen, Svend Erik; Svensson, Sigfrid; Koskikallio, Jouko; Kachi, Sukeji

doi:10.3891/acta.chem.scand.25-2768

Cited by 7 publications

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“…They compared the titration curve of the hypothetical ampholyte with s = 1 and with pKs at 7 k log 2 with those of two monovalent protolytes, one acid and one base, with the same pRs. This was in agreement with the well-established opinion put forward by authorities such as MacInnes and Ricci, and also strengthened by the present author [17], that log 4 is the least possible ApR that can occur in a bivalent protolyte. The author is as surprised as any reader of this article that intrinsic dissociation constants turn out to be directly responsible for titration and buffer capacity curves.…”

Section: Comparison With Previous Literaturesupporting

confidence: 93%

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On the use of dimensionless parameters in acid‐base theory: VI. The buffer capacities of equimolar binary mixtures of monovalent weak protolytes as compared to that of bivalent protolytes

Rilbe

1994

Electrophoresis

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The general equation for the relative molar buffer capacity, earlier shown to be valid for bivalent acids, bases, and ampholytes, is shown to hold also for equimolar, binary mixtures of monovalent protolytes if only the parameter s = square root of K1'/4K2' is exchanged for t = s + 1/4s. The same applies to the equations for the mean valence of the two classes of protolytes. As a consequence thereof, the titration and buffer capacity curves of a bivalent protolyte are identical with those of a monovalent protolyte with a pK' value equal to the with those of a monovalent protolyte with a pK' value equal to square root of K1'K2' of the bivalent one (the isoprotic point of an ampholyte). For a hypothetical bivalent acid, base, or ampholyte with s = 1, delta pK' = log 4, this implies that the intrinsic rather than the hybrid dissociation constants are responsible for the titration and buffer capacity curves.

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Section: Comparison With Previous Literaturesupporting

confidence: 93%

“…Thus the identity between Eqs. (12) and (17) and the corresponding equations for bivalent protolytes becomes natural and understandable if one accepts the view that the intrinsic dissociation constants are those which dictate the courses of the titration and buffer capacity curves. This also explains why b in Fig.…”

Section: The04mentioning

confidence: 99%

On the use of dimensionless parameters in acid‐base theory: VI. The buffer capacities of equimolar binary mixtures of monovalent weak protolytes as compared to that of bivalent protolytes

Rilbe

1994

Electrophoresis

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Can amphoteric substances with very small (or negative) ΔpK difference exist and what properties would their water solutions exhibit?

Stoyanov

Righetti

1998

Electrophoresis

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For calculating the buffer capacity of ampholytes an application of the stepwise and parallel dissociation models is considered. It is demonstrated that the scheme with stepwise dissociation possesses a "nonadditive" sum, i.e., the resulting buffer capacity of the system exceeds the sum of the separate contributions of the two ionogenic groups. On the other hand, the scheme of parallel dissociation is free from this disadvantage, and thus does not impose any restriction on the deltapK parameter.

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pK determinations via pH-mobility curves obtained by isoelectric focusing-electrophoresis: Theory and experimental verification

Valentini¹,

Gianazza

Righetti

1980

Journal of Biochemical and Biophysical Methods

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Isoelectric Fractionation, Analysis, and Characterization of Ampholytes in Natural pH Gradients. II. Buffering Capacity and Conductance of Isoionic Ampholytes. A Correction.

Cited by 7 publications

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On the use of dimensionless parameters in acid‐base theory: VI. The buffer capacities of equimolar binary mixtures of monovalent weak protolytes as compared to that of bivalent protolytes

On the use of dimensionless parameters in acid‐base theory: VI. The buffer capacities of equimolar binary mixtures of monovalent weak protolytes as compared to that of bivalent protolytes

Can amphoteric substances with very small (or negative) ΔpK difference exist and what properties would their water solutions exhibit?

pK determinations via pH-mobility curves obtained by isoelectric focusing-electrophoresis: Theory and experimental verification

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