Isoelectric Focusing of Seed Proteins

Wrigley, C.W.

doi:10.1007/978-1-4613-4181-9_8

Cited by 4 publications

(1 citation statement)

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“…Isoelectric focusing in 10% polyacrylamide gel was performed according to the method of Wrigley (8), in an MHA water-cooled apparatus at 4"C, in the pH 3 to 10 ampholine range. Gels were stained with Coomassie brilliant blue (9), or cut into 2-mm sections, shaken for 24 hr in 0.3 ml of normal saline in sealed tubes under nitrogen before measuring pH and enzyme activity.…”

Section: Gel Isoelectric Focusingmentioning

confidence: 99%

Radioimmunoassay of Human Ligandin

et al. 1983

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A sensitive and specific radioimmunoassay for human ligandin has been developed and used to study ligandin release into the serum in acute and chronic hepatitis. Serum ligandin concentrations were elevated in 67 of 68 cases of acute viral hepatitis. Ligandin levels frequently returned to normal within the first 2 weeks of illness. The rapid disappearance of ligandin preceded the return to normal of serum SGOT. In chronic active hepatitis, serum ligandin levels correlated significantly (p < 0.01) with histologic severity of disease. This correlation was not seen with SGOT. Serum ligandin may be useful in monitoring progress and the need for therapy in chronic active hepatitis.Ligandin, a basic protein (PI 8.9 to 9.3) found in abundance in mammalian liver, proximal renal tubules, and the small intestine, has been shown to have the enzymatic activity and the ability to bind nonsubstrate ligands, characteristic of glutathione S-transferase (GSH-T) (EC.2.5

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Section: Gel Isoelectric Focusingmentioning

confidence: 99%