Radioimmunoassay of Human Ligandin

Sherman, Morris; Bass, Nathan M.; Campbell, J. A.; Kirsch, Ralph E.

doi:10.1002/hep.1840030205

Cited by 30 publications

(3 citation statements)

References 14 publications

(4 reference statements)

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“…Alpha-GST were sporadically or persistently increasedeven in the absence of other factors capable of inducing a-GST [drugs, alcohol abuse, or toxic substances (7,9)] -in about 60% of the HCV-RNA-positive patients defined as apparently healthy carriers and in none of the HCV-RNAnegative subjects. These subjects showed a persistent nomality of ALT and y-GT levels; however, in some pathological hepatic conditions, the utility of ALT determination for monitoring liver damage has been questioned.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the increment of a-GST may be considered an early index of acute damage and their short half-life might allow a more direct monitoring of the duration of damage. Plasma a-GST levels are also markers of activity involving the centrolobular zone considered to be the principal site of the detoxifying system for toxic substances (hypoxia, xenobiotics, alcohol, and drugs), as well as for virus-mediated damage (7)(8)(9). Usually, ALT determination is employed to evaluate liver dam-age; however, no correlation exists between plasma ALT levels and liver histology, and ALT may also be normal for a long time when histologically documented liver damage occurs (10).…”

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confidence: 99%

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Determination of plasma alpha‐glutathione S‐transferases in patients with HCV‐related chronic infection: its significance and possible clinical relevance

Loguercio

Tuccillo

Caporaso

et al. 1998

Liver

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nation of plasma alpha-glutathione S-transferases in patients with HCVrelated chronic infection: its significance and possible clinical relevance.Abstract: Aims/Background: Alpha-glutathione S-transferases (a-GST) are the cytoplasmatic class of enzymes responsible for cellular detoxifying processes. We evaluated the plasma a-GST activity in relation to chronic infection caused by hepatitis C virus (HCV). Methods: Eighteen anti-HCV-negative healthy subjects (controls), 32 anti-HCV-positive subjects with a constant normality of alanine aminotransferases (ALT) and gamma-glutamyl transpeptidase (y-GT) levels ("apparently healthy carriers"), and 85 patients with HCV-related chronic liver disease (40 chronic hepatitis, 27 cirrhosis, and 18 with hepatocellular carcinoma) were studied. We assayed plasma a-GST in all subjects upon their entry into the study; and every 6 months for 3 years in the control group and in anti-HCV apparently healthy carriers. Results: Alpha-GST values were significantly higher than normal values in 57% of the 21 HCV-RNA-positive apparently healthy carriers and in none of 11 persistently HCV-RNAnegative subjects; the highest increment of a-GST was documented in patients with chronic hepatitis. We did not observe correlation among HCV-RNA, histological activity, y-GT and ALT or a-GST values. Conclusions: Therefore, the increment of plasma a-GST indicates a liver involvement even when ALT levels are normal. This may be clinically relevant to "apparently healthy carriers" whose plasma a-GST values. when 1 increased, might need further evaluation.Alpha-glutathione S-transferases (a-GST) are the "basic" class of a large enzyme family; they are ubiquitously distributed in the cells, particularly in the hepatocytes. Their biological functions are to transport hepatic anions, to conjugate glutathione (GSH) with various electrophiles and hydroperoxides, to synthesize prostaglandins and leukotrienes, and to activate selenium-independent glutathioneperoxidase (1-4).Alpha-GST are distributed throughout the liver lobule, represent about 5% of the cytosol in the hepatocytes and quickly rise in the plasma after liver damage. Their half-life iseabout 60 min, and when increased, plasma levels return to normal in about 5 days. Conversely, alanine aminotransferCarmela Loguerciol ,

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Determination of plasma alpha‐glutathione S‐transferases in patients with HCV‐related chronic infection: its significance and possible clinical relevance

Loguercio

Tuccillo

Caporaso

et al. 1998

Liver

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show abstract

“…Estudos imunohistoquímicos demonstram distribuição homogênea pelo parênquima hepático (Hiley et al, 1988), região periportal e centro lobular, sendo a última a zona mais segura de desintoxicação de álcool, drogas, hipóxia, assim como as lesões mediadas por vírus (Sherman et al, 1983;Harrison et al, 1990;Trull et al, 1994).…”

Section: Alpha Glutationa S Transferaseunclassified

Alpha Glutationa S Transferase: marcador de lesão hepática em pacientes com hepatite pelo vírus C?

Junior¹,

de²

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Ferraz que acreditaram em mim, quando eu era apenas mais um médico vindo do norte para estudar. Ao Prof Dr. Raymundo Soares de Azevedo Neto, coordenador da pósgraduação do Departamento de Patologia da FMUSP, pelo auxílio e compreensão nos momentos difíceis. Às secretárias Liduvina Barros e Vera Noya, pela ajuda constante. Ao Prof. Alex, pelo auxílio indispensável e incondicional na análise estatística. Aos pacientes e doadores de sangue que contribuíram, para que este trabalho fosse realizado.

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A review of new approaches to assessing hepatic function in animals

Cornelius

1987

Vet Res Commun

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Although a multitude of effective liver function tests are available for use in animals, a variety of modifications of currently used tests have been recently reported. In addition, newer procedures now used in human medicine may also provide unique insights into assessing and detecting acquired hepatic disorders in animals. Examples of new procedures are: assessing microsomal mixed function oxidase activity by plasma caffeine clearance; estimating the extent of active hepatic fibrogenesis through serum procollagen-III peptide levels; determining hepatic blood flow and functional mass by the galactose elimination capacity; detecting primary hepatocellular cancer through serum or urinary ligandin levels; and to estimate the liver's maximal capacity to excrete indocyanine green independent of blood flow. In evaluating drugs as to their hepatotoxicity, function tests should be included in the liver profile which measure specific metabolic alterations unique to the compound under study.

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Radioimmunoassay of Human Ligandin

Cited by 30 publications

References 14 publications

Determination of plasma alpha‐glutathione S‐transferases in patients with HCV‐related chronic infection: its significance and possible clinical relevance

Determination of plasma alpha‐glutathione S‐transferases in patients with HCV‐related chronic infection: its significance and possible clinical relevance

Alpha Glutationa S Transferase: marcador de lesão hepática em pacientes com hepatite pelo vírus C?

A review of new approaches to assessing hepatic function in animals

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