Isoelectric focusing of bacteria: Species location within an isoelectric focusing column by surface charge

Longton, Robert W.; Cole, John S.; Quinn, P.F.

doi:10.1016/0003-9969(75)90161-2

Cited by 36 publications

(17 citation statements)

References 13 publications

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“…We calculated the pI of E. coli between the pI markers 3.9 and 5.3 as 4.3. In [32,34] the pI of E. coli was found in the range of 4.2-6.4 by direct CIEF experiments. According to the microfluidic experiments presented in [52] pI value of E. coli was found less than 3.…”

Section: Cief Of Mixed Cultures Of Microorganismsmentioning

confidence: 98%

“…Their isoelectric points are practically independent on the experimental measurement conditions [29,30]. Recently, several papers were published showing successful separation of microorganisms according their pI by CIEF [4,27,28,[31][32][33][34]. It is easy possible to quantify on-line mixed bacterial cultures by electrophoretic techniques [25,35].…”

Section: Introductionmentioning

confidence: 99%

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Sol‐gel column technology for capillary isoelectric focusing of microorganisms and biopolymers with UV or fluorometric detection

et al. 2003

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The sol-gel surface modification is used for capillary isoelectric focusing of microorganisms and biopolymers. The coating strongly decreases the electroosmotic flow so that it enables the use of the short capillaries down to 100 mm in the separation length. The examples of capillary isoelectric focusing of the low-molecular-mass pI markers and mixed cultures of microbial populations of Escherichia coli, Candida albicans, Staphylococcus epidermidis, and Enteroccocus faecalis with UV detection are shown. It is possible to quantify bacterial cells according to their peak areas; the minimum detectable number of microbial cells was 5 x 10(2)-1 x 10(3). The compatibility of sol-gel capillaries with sensitive fluorometric detection of fluorescent pI markers together with fluorescein labeled proteins is demonstrated.

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Section: Cief Of Mixed Cultures Of Microorganismsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Sol‐gel column technology for capillary isoelectric focusing of microorganisms and biopolymers with UV or fluorometric detection

et al. 2003

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“…Those pI values derived from EPM vs. pH measurements were all less than 3 over a range of ionic strengths [8,11,17,29]. Earlier experiments that measured the pI of E. coli by direct IEF experiments (performed in density gradients with Ampholine buffers) found the pI to be much higher, from 4.2 to 6.4 [3,10] …”

Section: Isoelectric Point Of E Herbicolamentioning

confidence: 99%

“…In theory, particles after treatment with these techniques can remain in their native state. In practice, experiments have shown that a significant fraction of the initial bacterial population remained viable after either ZE [2] or IEF [3]. Reducing the size of ZE and IEF equipment can result in decreasing both power consumption and run-time.…”

Section: Introductionmentioning

confidence: 99%

“…However, use of ZE and IEF as a practical tool for the concentration and/or fractionation of bacterial samples has received considerably less attention. Typically, such reports required a density gradient and 12±24 h [3,10,12], or were performed in capillaries [2,13,14]; all of which were operated in batch mode at high applied voltages (0.3±20 kV). Recent application of carrier-free capillary isoelectric focusing (CIEF) to fractionation of a heterogeneous bacterial sample resulted in excellent separation among three different Gram-negative bacteria after~3 min [13], proving that such samples are quite amenable to electrokinetic-based separations.…”

Section: Introductionmentioning

confidence: 99%

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Continuous concentration of bacteria in a microfluidic flow cell using electrokinetic techniques

2001

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A novel method for the concentration of bacterial solutions is presented that implements electrokinetic techniques, zone electrophoresis (ZE) and isoelectric focusing (IEF), in a microfluidic device. The method requires low power (< 3e-5 W) and can be performed continuously on a flowing stream. The device consists of two palladium electrodes held in a flow cell constructed from layers of polymeric film held together by a pressure-sensitive adhesive. Both ZE and IEF are performed with carrier-free solutions in devices in which the electrodes are in intimate contact with the sample fluid. IEF experiments were performed using natural pH gradients; no carrier ampholyte solution was required. Experiments performed in buffer alone resulted in significant electroosmotic flow. Pretreatment of the sample chamber with bleach followed by a concentrated solution of cationic detergent effectively suppressed electroosmotic flow.

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Bacterial Cell Walls and Surfaces

Wicken

1985

Bacterial Adhesion

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Isoelectric focusing of bacteria: Species location within an isoelectric focusing column by surface charge

Cited by 36 publications

References 13 publications

Sol‐gel column technology for capillary isoelectric focusing of microorganisms and biopolymers with UV or fluorometric detection

Sol‐gel column technology for capillary isoelectric focusing of microorganisms and biopolymers with UV or fluorometric detection

Continuous concentration of bacteria in a microfluidic flow cell using electrokinetic techniques

Bacterial Cell Walls and Surfaces

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