Isocyanate-terminated urethane-based methacrylate for in situ collagen scaffold modification

Fan, Yu; Xu, Rongchen; Huang, Li; Luo, M.L.; Li, J.; Tay, Franklin R.; Niu, Li Na; Chen, Ji Hua

doi:10.1016/j.msec.2020.110902

Cited by 15 publications

(11 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Presumably, the aldehydes bind covalently to the functional domains of gelatinases such as the cysteine residues (sulfhydryl groups) in the prodomains relevant to the activation and stability of enzymes (Liu and Khalil 2017; Laronha and Caldeira 2020). FA inhibits collagenolytic activity by immobilizing the gelatinase molecules or inducing irreversible changes in the active domains (Yu, Xu, et al 2020). In addition, FA improves the enzymatic resistance of the dentin collagen matrix by covalently binding to the collagen fibers (Yu et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Effect of Collagen-Reactive Functional Monomer on Etch-and-Rinse Adhesives

Tian

et al. 2022

J Dent Res

View full text Add to dashboard Cite

In this study, we evaluated a novel functional monomer (4-formylphenyl acrylate [FA]) that can specifically and covalently bind to the dentin collagen matrix as a potential alternative hydrophobic diluent-like monomer for improving the durability of dentin bonding. Experimental adhesives with different FA contents (0%, 10%, 20%, and 30%) were evaluated as partial substituents for the hydrophilic monomer 2-hydroxyethyl methacrylate, with the commercial adhesive One-Step (Bisco, Inc.) employed as the positive control. Their degree of conversion, viscosity, hydrophobicity, mechanical properties, and water absorption/solubility were measured as the comprehensive characterization. In situ zymographic assays were performed to determine the extent to which FA inhibits the endogenous hydrolytic activity of dentin. Finally, the bonding performances of the novel adhesives were evaluated with microtensile strength tests and scanning electron microscopy. The results showed that the incorporation of FA significantly improved the mobility of experimental adhesives attributable to the dilution property of FA. In contrast to the possible compromised rate of polymerization by hydroxyethyl methacrylate, FA exhibited typical characteristics of favorable copolymerization with polymerizable monomers in adhesives and improved the degree of conversion of experimental adhesives. The rigidity and hydrophobic properties of the phenyl framework of the FA molecule conferred superior mechanical properties and hydrolysis resistance to the novel experimental adhesives. An inhibitory effect on gelatinolytic activities within the hybrid layer was also observed in the in situ zymographic assays, even at a low FA concentration (10%). In conjunction with the significantly improved infiltration found via scanning electron microscopy, the experimental adhesives containing FA possessed significantly better-maintained microtensile strength, even after aging. Thus, the incorporation of this novel monomer endowed the experimental adhesives with multiple enhanced functionalities. These remarkable advantages highlight the suitability of the monomer for further applications in clinical practice.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Collagen-Reactive Functional Monomer on Etch-and-Rinse Adhesives

Tian

et al. 2022

J Dent Res

View full text Add to dashboard Cite

show abstract

“…To evaluate the binding affinity of CLM to dentin collagen, molecular docking was performed using Molecular Operating Environment (Chemical Computing Group, Montreal, QC, Canada) [ 34 , 42 ]. Three crystal structures of type I collagen were selected from the Protein Data Bank database (IDs: 4OY5, 1CGD, and 1QSU).…”

Section: Methodsmentioning

confidence: 99%

Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding

Hu¹,

et al. 2023

Materials Today Bio

View full text Add to dashboard Cite

“…We first studied the modification effects of TiO 2 NSs on the physicochemical properties of ARCs, which could basically regulate the stability and bioactivity of MTFs [37,38]. The properties include DC, surface elastic module, hardness, thermal stability, water solubility (WSL), water sorption (WSp), surface wettability, and roughness [39][40][41][42][43].…”

Section: Physicochemical Properties Of Tio 2 -Arcsmentioning

confidence: 99%

Enhancing bioactivity and stability of polymer-based material-tissue interface through coupling multiscale interfacial interactions with atomic-thin TiO2 nanosheets

et al. 2022

Nano Res.

View full text Add to dashboard Cite

Stable and bioactive material—tissue interface (MTF) basically determines the clinical applications of biomaterials in wound healing, sustained drug release, and tissue engineering. Although many inorganic nanomaterials have been widely explored to enhance the stability and bioactivity of polymer-based biomaterials, most are still restricted by their stability and biocompatibility. Here we demonstrate the enhanced bioactivity and stability of polymer-matrix bio-composite through coupling multiscale material—tissue interfacial interactions with atomically thin TiO 2 nanosheets. Resin modified with TiO 2 nanosheets displays improved mechanical properties, hydrophilicity, and stability. Also, we confirm that this resin can effectively stimulate the adhesion, proliferation, and differentiation into osteogenic and odontogenic lineages of human dental pulp stem cells using in vitro cell—resin interface model. TiO 2 nanosheets can also enhance the interaction between demineralized dentinal collagen and resin. Our results suggest an approach to effectively up-regulate the stability and bioactivity of MTFs by designing biocompatible materials at the sub-nanoscale. Electronic Supplementary Material Supplementary material (further details of fabrication and characterization of TiO 2 NSs and TiO 2 -ARCs, the bioactivity evaluation of TiO 2 -ARCs on hDPSCs, and the measurement of interaction with demineralized dentin collagen) is available in the online version of this article at 10.1007/s12274-022-5153-1.

show abstract

Isocyanate-terminated urethane-based methacrylate for in situ collagen scaffold modification

Cited by 15 publications

References 35 publications

Effect of Collagen-Reactive Functional Monomer on Etch-and-Rinse Adhesives

Effect of Collagen-Reactive Functional Monomer on Etch-and-Rinse Adhesives

Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding

Enhancing bioactivity and stability of polymer-based material-tissue interface through coupling multiscale interfacial interactions with atomic-thin TiO2 nanosheets

Contact Info

Product

Resources

About