Isocratic high-performance liquid chromatography (HPLC) for simultaneous quantification of curcumin and piperine in a microparticle formulation containing Curcuma longa and Piper nigrum

Abstract

Poor bioavailability has been reported as a major challenge in the development of curcumin as a pharmaceutical agent. However, co-administration of curcumin with piperine has been shown to improve curcumin bioavailability. Therefore, to assure product control quality, an analytical method needs to be developed for the determination of curcumin and piperine content in a dosage form formulation. The objective of this study was to develop a simple isocratic reversed-phase HPLC (RP-HPLC) method to simultaneously quantify curcumin and piperine content in solid dispersion based microparticle formulation containing Curcuma longa and Piper nigrum extracts. The method was validated according to the International Council for Harmonization (ICH) guideline. Chromatographic separation of three curcuminoids and piperine could be achieved using acetonitrile-methanol-water of 65:5:35 %, at a flow rate of 1 mL/min and a wavelength of 353 nm for detection. Resolution (Rs) of 3.57 and 1.68 for piperine and curcumin, respectively, a theoretical plate number (N) > 8000 and a tailing factor (T) < 1.5 indicate a satisfactory separation of the compounds. The calibration curve was linear from 1.25-15 μg/mL and 2.5–30 μg/mL for piperine and curcumin, respectively, with the correlation coefficient of >0.999. The intra-day/inter-day accuracy and precision demonstrated a recovery of 99.54–101.50%/99.38–99.89% and 100.78–102.51%/101.15–102.47% with a Relative Standard Deviation (RSD) of 0.53–0.95%/0.13–1.44 % and 0.28–1.62%/0.46–1.14% for piperine/curcumin. The limit of detection (LOD) were 0.27 and 0.42 μg/mL, for piperine and curcumin, which reveals an adequate sensitivity. A solid dispersion based microparticle formulation containing C. longa and P. nigrum extracts confirmed the validity of the developed method as a recovery of 91.14% and 99.14% for piperine and curcumin, respectively. In conclusion, all the tested parameters confirm the precision, accuracy, and reliability of the method for the simultaneous analysis of curcumin and piperine within a microparticle formulation containing C. longa and P. nigrum extracts.

Formulation of Piperine Nanoparticles: In Vitro Breast Cancer Cell Line and In Vivo Evaluation

“…The aliquots (5 mL) were taken at pre-decided time points (i.e., just after 15, 30, 45, 60, 75, 90, 105, 120, 135, 150, 165, and 180 min) and replaced with an equal volume of fresh release media to maintain the volume of the medium. In the end, the concentration of permeated PPN from the samples was measured as per the reported RP-HPLC method [ 36 ]. For RP-HPLC analysis, a C 18 column (250 ± 4.6 mm, Eurospher 100 with 5 μm) was used for chromatographic separation of PPN.…”

“…The supernatant was removed and diluted properly with the mobile phase. Then, the samples were evaluated in the plasma at each time point by RP-HPLC [ 36 ]. Finally, different pharmacokinetic parameters were calculated from the plasma PPN concentration and time profiles.…”

Effect of Co-Administration of Curcumin with Amlodipine in Hypertension

“…Therefore, it was thought to be a statistical error caused by the difference in the baseline blood pressure values of the individual. As such, the effect of co-administration was better in ex vivo, but the reason there were no differences in in vivo was presumed to be the poor bioavailability of curcumin due to its poor absorption, rapid systemic elimination [ 36 ], and high rate of metabolism [ 23 ].…”

“…Since curcumin also relaxes blood vessels and lowers blood pressure, it might have beneficial effects when administered in combination with curcumin. The co-administration of curcumin and piperine, an alkaloid compound of black pepper ( P. nigrum ), was found to improve the bioavailability of curcumin [ 23 ], and the co-administration of curcumin and arabinogalactan was found to induce the apoptosis of breast cancer cells and inhibit tumor growth through p53 overexpression [ 24 ]. However, there have been no studies on the effects of the co-administration of curcumin and amlodipine on hypertension.…”

Determination of Curcuminoids, Piperine, Boswellic Acids and Andrographolides in Food and Dietary Supplements by HPLC

“…piperine have been developed (19,20), albeit not mentioning or determining BDMC and DMC. HPLC and HPTLC methods for determination of curcumin and α-and β-boswellic acids (21) and curcumin, PIP and boswellic acid (22) were found, but for the former, BDMC and DMC, as well as other boswellic acids are not mentioned, while for the latter separation of the components of each substance group was not achieved.…”