Islet Cell Transplantation

Reemtsma, K; Giraldo, Nelson; Depp, David A.; Eichwald, E. J.

doi:10.1097/00000658-196809000-00011

Cited by 19 publications

(4 citation statements)

References 9 publications

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“…3 In 1972 Lee described a new method which included end-to-side anastomoses and the removal of a segment of the donor's aorta along with not only the celiac axis but the mesenteric artery as well.5 The reason for this was to maintain pancreatic vascularization using the lower pancreatic-duodenal vessels.…”

Section: Discussionmentioning

confidence: 99%

Pancreas Transplantation in the Rat Using Lee's Technique: A Reliable Model in Experimental Microsurgery

Cataldo

Puleo

et al. 1989

Journal of Investigative Surgery

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Numerous unresolved problems, both technical and immunological, in pancreas transplantation stimulate experimental studies. Dogs have been routinely used in experimental studies but today rats are more commonly used. However, pancreas transplantation in the rat presents complex technical problems and requires a good knowledge of microsurgical techniques. In 1983 Squifflet undertook an experimental study aimed at evaluating the technical aspects of pancreas transplantation in the rat and calculating the success rates using different methods. The comparison of four methods revealed to our surprise that 100% of the rats operated on using Lee's technique had complications, with a 0% survival rate. In our study we report our experience using Lee's technique which we had the opportunity of mastering directly under the supervision of Professor Lee. We performed 100 pancreas transplantations using Lee's technique and divided our study in two phases. In the first phase we performed 70 pancreas transplantations and overall survival, after 1 week, was 42 rats (60%). In the second phase on 30 rats diabetes was induced by administering 70 mg/kg of streptozotocin. These 30 diabetic rats underwent pancreas transplantation and overall survival, after 1 week, was 25 (83.3%). We believe that our successful survival rates could probably be explained by the close collaboration between Lee and our department. Moreover, we noted the importance of constant training in obtaining better results, and in our opinion Lee's technique of pancreas transplantation is a reliable experimental model which can be used to resolve problems linked to pancreas transplantation.

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Section: Discussionmentioning

confidence: 99%

Pancreas Transplantation in the Rat Using Lee's Technique: A Reliable Model in Experimental Microsurgery

Cataldo

Puleo

et al. 1989

Journal of Investigative Surgery

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“…Whether this theoretical advantage would outweigh the very real problems of genetic disparity is unanswered. From an historical standpoint, the earliest examples of islet cell transplants involved xenogeneic, rather than allogeneic tissue [7,8].…”

Section: Replacementmentioning

confidence: 99%

Address by the Honorary Founding President of the Xenotransplantation SocietyThe Eye of an Eagle: xenobiology and the quest for bioadvantage

Reemtsma

2000

Xenotransplantation

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“…Rodent models are genetically well defined and are superior to large animals for immunological studies. In 1968, Reemtsma et al were the first to report a ductligated rat model. ' In the early 1970s Lee et al described a rat pancreaticoduodenal transplant which drained into the recipient They continued to develop the model with drainage ultimately into the recipient bladder.4 A long warm ischaemic time and clamping the systemic circulation for long periods of time combined with the lengthy training necessary for adequate microsurgical skil15s6 led to the use of cuff techniques for anastomoses using aortic interposition for PTx in the rat.5…”

Section: Introductionmentioning

confidence: 99%

Successful Methods of Pancreas Transplantation in the Rat Using a Cuff Technique

Kobayashi

Kamada

Toyama

et al. 1994

Australian and New Zealand Journal of Surgery

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Two models of pancreas transplantation were examined in the rat with a view to choosing one for regular use in functional experiments. A cuff technique applied to the renal vessels of the recipient was used. The histology and endocrine functioning of whole pancreas-duodenal transplant (Tx) plus bladder drainage (drainage model), and the pancreatic duct ligated segmental pancreas Tx (ligated model) were examined. Syngeneic operations were performed using either Lewis or Wistar rats. Streptozotocin (STZ) 60mg/kg intravenously (i.v.) was used to render the recipient rats diabetic. A cuff technique was used with both models to anastomose the grafts to the renal vessels instead of the conventional technique of hand suturing to the abdominal vessels. This allows a shorter warm ischaemia time for the donor pancreas and leaves the systemic circulation intact leading to a high success rate for both techniques. Operation survival rates were 93% (n = 30) and 90% (n= 10) in the ligated and drainage models, respectively. The recipients in both groups were normoglycaemic for > 100 days. Histological examination revealed atrophic exocrine tissue early in the ligated group but only two from the drainage group showed exocrine atrophy at > 100 days. There was no statistical difference in i.v. glucose tolerance tests with both models showing a normal pattern. Thus, endocrine function remained independent of exocrine function. Both models are quicker than the conventional techniques. The duct ligation model was a simpler transplant to perform, suggesting that this should be the technique of choice in future experiments.

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Islet Cell Transplantation

Cited by 19 publications

References 9 publications

Pancreas Transplantation in the Rat Using Lee's Technique: A Reliable Model in Experimental Microsurgery

Pancreas Transplantation in the Rat Using Lee's Technique: A Reliable Model in Experimental Microsurgery

Address by the Honorary Founding President of the Xenotransplantation SocietyThe Eye of an Eagle: xenobiology and the quest for bioadvantage

Successful Methods of Pancreas Transplantation in the Rat Using a Cuff Technique

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