Ischemic Stroke: New Neuron Recovery Approach with Mesenchymal and Neural Stem Cells

Chouw, Angliana; Triana, Rina; Dewi, Nurrani Mustika; Darmayanti, Siska; Rahman, Miftakh Nur; Susanto, Ardian; Putera, Bayu Winata; Sartika, C.R.

doi:10.21705/mcbs.v2i2.28

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…Ischemic stroke is more common than hemorrhagic stroke, with a prevalence of 67.1% of all stroke cases. 3 Ischemic stroke is one of the most common causes of death or results in disabilities, including cognitive function. The prevalence of post-stroke cognitive impairment is 20-80%.…”

Section: Introductionmentioning

confidence: 99%

Chlorogenic Acid Protects Cell Death in the Cerebellum through Anti-Apoptotic Protein Bcl2 in Transient Global Ischemia Cases

Hermawati,

Handini,

Ilmiawan

et al. 2024

Mol Cell Biomed Sci

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Background: Cerebellum is one of the vital components of the brain that will be affected by ischemia-reperfusion (IR) injury. IR injury will increase free radicals, which in turn can trigger apoptosis and cell death. Therefore, this study was conducted to examine the effect of chlorogenic acid administration on apoptosis and the number of cells in the cerebellum of rats with global ischemic transients.Materials and methods: Wistar rats were divided into five groups: sham-operated (C1), IR (C2), IR + 15 mg/kgBW chlorogenic acid (T1), IR + 30 mg/kgBW chlorogenic acid (T2), and IR + 60 mg/kgBW chlorogenic acid (T3). C2, T1, T2, and T3 groups received bilateral common carotid occlusion (BCCO) surgery to induce IR injury. Thirty minutes after BCCO surgery, T1, T2, and T3 rats were administered chlorogenic acid in various doses intraperitoneally. RNA extraction and real-time polymerase chain reaction (PCR) measurements were then performed on NeuN, Bcl2, Bax, caspase 3, as well as on GAPDH as housekeeping genes.Results: There were significant differences in NeuN expressions between groups, with the highest expression shown in C1 followed by T3. Bcl2 expressions were also significantly different between groups, and rats in C1 and T3 showed to be significantly higher compared to C2, while T1 was significantly lower than C1. However, Bax and caspase 3 expressions showed no significant differences.Conclusion: Chlorogenic acid in 60 mg/kgBW dose increases NeuN expression and Bcl2 mRNA expression after transient global ischemia. These increases might correlate with the heightened level of protection against apoptosis in the cerebellum, hence showing its potential in protecting neuron cells.Keywords: transient global ischemia, chlorogenic acid, cerebellum, apoptosis

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Section: Introductionmentioning

confidence: 99%

Chlorogenic Acid Protects Cell Death in the Cerebellum through Anti-Apoptotic Protein Bcl2 in Transient Global Ischemia Cases

Hermawati,

Handini,

Ilmiawan

et al. 2024

Mol Cell Biomed Sci

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“…Stem cells can treat various diseases and become an alternative treatment of COVID-19. 6 Mesenchymal stem cells (MSCs) are cells that have a high division rate and can differentiate into several cell types. 7 MSCs can be found in organs and tissues, such as umbilical cord, umbilical cord blood, adult connective tissue, placenta, amniotic membranes, dental tissue, and bone marrow.…”

Section: Introductionmentioning

confidence: 99%

Allogeneic Mesenchymal Stem Cells and Its Conditioned Medium as a Potential Adjuvant Therapy for COVID-19

Widowati

Faried

Kusuma³

et al. 2023

Mol Cell Biomed Sci

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Recent research has demonstrated that mesenchymal stem cells (MSCs) potentially benefit and enhance coronavirus disease (COVID-19) recovery. This benefit occurs via a mechanism that promotes viral clearance by phagocytes and macrophages. This action occurs through the innate (increase in IL-10 production and decrease in TNF-α and IL-12 production) and the adaptive immune system (decrease in IL-17 production, promote regulatory T cell proliferation and inhibit effectors T cell proliferation). MSCs are expected to act as an anti-inflammatory in the hyper-inflammatory state of COVID-19. MSCs enhance immune cell replacement that have been overwhelmed or have been lost due to cytokine storm. Although vaccines are the answer to this pandemic, MSCs can improve COVID-19 patients, especially in patients with chronic illnesses. The focus on keeping death-rates low is a great opportunity for MSCs-based therapy for severe or critically ill patients. MSCs and conditioned medium have the potential to serve as adjunctive therapy in preventing the body's overactive defense response or the so-called cytokine storm caused by COVID-19.Keywords: adjuvant therapy, COVID-19, mesenchymal stem cells, secretome

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Nanomaterial-based approach in stroke

Ghosh,

Datta,

Sarmah

et al. 2024

Natural Molecules in Neuroprotection and Neurotoxicity

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Ischemic Stroke: New Neuron Recovery Approach with Mesenchymal and Neural Stem Cells

Cited by 4 publications

References 35 publications

Chlorogenic Acid Protects Cell Death in the Cerebellum through Anti-Apoptotic Protein Bcl2 in Transient Global Ischemia Cases

Chlorogenic Acid Protects Cell Death in the Cerebellum through Anti-Apoptotic Protein Bcl2 in Transient Global Ischemia Cases

Allogeneic Mesenchymal Stem Cells and Its Conditioned Medium as a Potential Adjuvant Therapy for COVID-19

Nanomaterial-based approach in stroke

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