Ischemic postconditioning influences electron transport chain protein turnover in Langendorff-perfused rat hearts

Cao, Song; Lei, Yang; Wang, Haiying; Mao, Xiaole; Chen, Jincong; JiMing, Liu; Xia, Zhengyuan; Zhang, Lin; Liu, Xingkui; Yu, Tian

doi:10.7717/peerj.1706

Cited by 25 publications

(29 citation statements)

References 56 publications

(71 reference statements)

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“…Western blot analysis of total protein from c-kit + cell lysis was performed as described previously ( Cao et al, 2016 ). The protein extracts were separated by SDS-polyacrylamide gels electrophoresis (SDS-PAGE) and transferred to PVDF membranes.…”

Section: Methodsmentioning

confidence: 99%

miR-21 increases c-kit⁺ cardiac stem cell proliferation in vitro through PTEN/PI3K/Akt signaling

Shi

Deng

Long

et al. 2017

PeerJ

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The low survival rate of cardiac stem cells (CSCs) in the ischemic myocardium is one of the obstacles in ischemic cardiomyopathy cell therapy. The MicroRNA (miR)-21 and one of its target protein, the tensin homolog deleted on chromosome ten (PTEN), contributes to the proliferation of many kinds of tissues and cell types. It is reported that miR-21 promotes proliferation through PTEN/PI3K/Akt pathway, but its effects on c-kit+ CSC remain unclear. The authors hypothesized that miR-21 promotes the proliferation in c-kit+ CSC, and evaluated the involvement of PTEN/PI3K/Akt pathway in vitro. miR-21 up-regulation with miR-21 efficiently mimics accelerated cell viability and proliferation in c-kit+ CSC, which was evidenced by the CCK-8, EdU and cell cycle analyses. In addition, the over-expression of miR-21 in c-kit+ CSCs notably down-regulated the protein expression of PTEN although the mRNA level of PTEN showed little change. Gain-of-function of miR-21 also increased the phosphor-Akt (p-Akt) level. Phen, the selective inhibitor of PTEN, reproduced the pro-proliferation effects of miR-21, while PI3K inhibitor, LY294002, totally attenuated the pro-survival effect of miR-21. These results indicate that miR-21 is efficient in promoting proliferation in c-kit+ CSCs, which is contributed by the PTEN/PI3K/Akt pathway. miR-21 holds the potential to facilitate CSC therapy in ischemic myocardium.

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Section: Methodsmentioning

confidence: 99%

miR-21 increases c-kit⁺ cardiac stem cell proliferation in vitro through PTEN/PI3K/Akt signaling

Shi

Deng

Long

et al. 2017

PeerJ

Self Cite

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“…Western blot analysis of total protein from c-kit + cell lysis was performed as described previously [ 63 ]. The protein extracts were separated by SDS-polyacrylamide gels electrophoresis (SDS-PAGE) and transferred to PVDF membranes.…”

Section: Methodsmentioning

confidence: 99%

miR‐21 Reduces Hydrogen Peroxide‐Induced Apoptosis in c‐kit⁺ Cardiac Stem Cells In Vitro through PTEN/PI3K/Akt Signaling

Deng

Wang

Long

et al. 2016

Oxidative Medicine and Cellular Longevity

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The low survival rate of cardiac stem cells (CSCs) in the infarcted myocardium hampers cell therapy for ischemic cardiomyopathy. MicroRNA-21 (miR-21) and one of its target proteins, PTEN, contribute to the survival and proliferation of many cell types, but their prosurvival effects in c-kit+ CSC remain unclear. Thus, we hypothesized that miR-21 reduces hydrogen peroxide- (H2O2-) induced apoptosis in c-kit+ CSC and estimated the contribution of PTEN/PI3K/Akt signaling to this oxidative circumstance. miR-21 mimics efficiently reduced H2O2-induced apoptosis in c-kit+ CSC, as evidenced by the downregulation of the proapoptosis proteins caspase-3 and Bax and upregulation of the antiapoptotic Bcl-2. In addition, the gain of function of miR-21 in c-kit+ CSC downregulated the protein level of PTEN although its mRNA level changed slightly; in the meantime, miR-21 overexpression also increased phospho-Akt (p-Akt). The antiapoptotic effects of miR-21 were comparable with Phen (bpV), the selective inhibitor of PTEN, while miR-21 inhibitor or PI3K's inhibitor LY294002 efficiently attenuated the antiapoptotic effect of miR-21. Taken together, these results indicate that the anti-H2O2-induced apoptosis effect of miR-21 in c-kit+ CSC is contributed by PTEN/PI3K/Akt signaling. miR-21 could be a potential molecule to facilitate the c-kit+ CSC therapy in ischemic myocardium.

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“…ACT and Tub are cytoskeleton proteins (Zarrouk et al 2015 ), and ACT and Myo form the filaments of the muscle (Li et al 2016 ). SdhA is a component of electron transport chain complexes (Cao et al 2016 ). RP II catalyzes DNA transcription and synthesis of mRNA precursors (Sims et al 2004 ).…”

Section: Discussionmentioning

confidence: 99%

Identification and evaluation of reference genes in the Chinese white wax scale insect Ericerus pela

Yang

Sun

et al. 2016

SpringerPlus

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BackgroundThe Chinese white wax scale insect, Ericerus pela, is a well-known resource insect. The females and males are dramatically distinct at each developmental stage. We sought to identify suitable reference genes to use as internal controls in molecular research on E. plea.ResultsgeNorm, RefFinder and Normfinder analyses showed that ßTub-2 was the best reference gene throughout different developmental stages; SdhA-1 was the most stable reference gene in different tissues, and ßTub-1 was the most reliable reference gene under treatment with different temperatures. The results also showed that the optimal number of reference genes for analyzing target gene expression levels in the three experimental conditions was two.ConclusionsThe identified reference genes are suitable reference genes for normalization in RT-qPCR of E. pela samples.

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Ischemic postconditioning influences electron transport chain protein turnover in Langendorff-perfused rat hearts

Cited by 25 publications

References 56 publications

miR-21 increases c-kit⁺ cardiac stem cell proliferation in vitro through PTEN/PI3K/Akt signaling

miR-21 increases c-kit⁺ cardiac stem cell proliferation in vitro through PTEN/PI3K/Akt signaling

miR‐21 Reduces Hydrogen Peroxide‐Induced Apoptosis in c‐kit⁺ Cardiac Stem Cells In Vitro through PTEN/PI3K/Akt Signaling

Identification and evaluation of reference genes in the Chinese white wax scale insect Ericerus pela

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Ischemic postconditioning influences electron transport chain protein turnover in Langendorff-perfused rat hearts

Cited by 25 publications

References 56 publications

miR-21 increases c-kit+ cardiac stem cell proliferation in vitro through PTEN/PI3K/Akt signaling

miR-21 increases c-kit+ cardiac stem cell proliferation in vitro through PTEN/PI3K/Akt signaling

miR‐21 Reduces Hydrogen Peroxide‐Induced Apoptosis in c‐kit+ Cardiac Stem Cells In Vitro through PTEN/PI3K/Akt Signaling

Identification and evaluation of reference genes in the Chinese white wax scale insect Ericerus pela

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Product

Resources

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miR-21 increases c-kit⁺ cardiac stem cell proliferation in vitro through PTEN/PI3K/Akt signaling

miR-21 increases c-kit⁺ cardiac stem cell proliferation in vitro through PTEN/PI3K/Akt signaling

miR‐21 Reduces Hydrogen Peroxide‐Induced Apoptosis in c‐kit⁺ Cardiac Stem Cells In Vitro through PTEN/PI3K/Akt Signaling