Ischemic flap survival improvement by composition-selective fat grafting with novel adipose tissue derived product - stromal vascular fraction gel

Zhang, Pan; Feng, Jiaxuan; Liao, Yunjun; Cai, Junrong; Zhou, Tao; Sun, Mingliang; Gao, Jianhua; Gao, Xinbo

doi:10.1016/j.bbrc.2017.11.196

Cited by 30 publications

(22 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SEM demonstrated successful concentration of ECM fibers in the ECM/SVF-gel, which could thus provide a scaffolding biomaterial with the microenvironment required for MSCs renewal and differentiation. Consistent with previous studies, we found that ADSCs isolated from ECM/SVF-gel could proliferate and differentiate into various lineages (Zhang et al, 2018;, demonstrating that the ECM/SVF-gel preserved the stem cells within adipose tissue. Therefore, the ECM/SVFgel has attractive prospects as a natural scaffolding biomaterial and stem cell carrier to promote cartilage regeneration.…”

Section: Discussionsupporting

confidence: 92%

“…Based on this principle, researchers have developed nanofat from lipoaspirate grafting between two syringes, and micro-fragmented adipose tissue from a commercial device kit. Recently, these have shown attractive potential in regenerative medicine including promotion of wound healing (Yao et al, 2017;Lonardi et al, 2019), improving ischemic flap survival (Zhang et al, 2018), and remolding bone tissue formation (Guerrero et al, 2018). However, it is still unclear whether autologous fractionated adipose tissue can provide new treatment options to repair articular cartilage defects.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Autologous Fractionated Adipose Tissue as a Natural Biomaterial and Novel One-Step Stem Cell Therapy for Repairing Articular Cartilage Defects

Zhao

Zong

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Articular cartilage damage remains a tough challenge for clinicians. Stem cells have emerged promising biologics in regenerative medicine. Previous research has widely demonstrated that adipose-derived mesenchymal stem cells (ADSCs) can promote cartilage repair due to their multipotency. However, enzymatic isolation and monolayer expansion of ADSCs decrease their differentiation potential and limit their clinical application. Here, a novel adipose tissue-derived product, extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel), was obtained by simple mechanical shifting and centrifugation to separate the fat oil and concentrate the effective constituents. This study aimed to evaluate the therapeutic effect of this natural biomaterial on the repair of articular cartilage defects. Scanning electron microscopy showed that the fibrous structure in the ECM/SVF-gel was preserved. ADSCs sprouted from the ECM/SVFgel were characterized by their ability of differentiation into chondrocytes, osteoblasts, and adipocytes. In a rabbit model, critical-sized cartilage defects (diameter, 4 mm; depth, 1.5 mm) were created and treated with microfracture (MF) or a combination of autologous ECM/SVF-gel injection. The knee joints were evaluated at 6 and 12 weeks through magnetic resonance imaging, macroscopic observation, histology, and immunohistochemistry. The International Cartilage Repair Society score and histological score were significantly higher in the ECM/SVF-gel group than those in the MFtreated group. The ECM/SVF-gel distinctly improved cartilage regeneration, integration with surrounding normal cartilage, and the expression of hyaline cartilage marker, type II collagen, in comparison with the MF treatment alone. Overall, the ready-touse ECM/SVF-gel is a promising therapeutic strategy to facilitate articular cartilage regeneration. Moreover, due to the simple, time-sparing, cost-effective, enzyme-free, and minimally invasive preparation process, this gel provides a valuable alternative to stem cell-based therapy for clinical translation.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Autologous Fractionated Adipose Tissue as a Natural Biomaterial and Novel One-Step Stem Cell Therapy for Repairing Articular Cartilage Defects

Zhao

Zong

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Adipose tissues, as well as their derivatives, including nanofat, ADSCs, SVF, and SVF-gel, have been used in the treatment of many ischemic disorders in different animal models [ 1 , 29 , 30 , 32 , 35 – 37 ]. The protective effects of those materials are believed to be mainly related to their cellular components via paracrine growth factors.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have also shown that, when centrifuged, nanofat consists of an oil layer, a cellular/extracellular matrix fraction, and a liquid fraction [ 29 – 31 ]. The cellular/extracellular matrix fraction, known as “SVF-gel”, is composed of a high concentration of ADSCs and a functional extracellular matrix that could promote angiogenesis in ischemic tissues [ 29 , 30 , 32 ]. Unfortunately, the liquid fraction has been ignored in previous studies.…”

Section: Introductionmentioning

confidence: 99%

Fat extract promotes angiogenesis in a murine model of limb ischemia: a novel cell-free therapeutic strategy

et al. 2018

View full text Add to dashboard Cite

BackgroundThe proangiogenic capacity of adipose tissue and its derivatives has been demonstrated in a variety of studies. The paracrine mechanism of the cellular component is considered to play a critical role in the regenerative properties of these tissues. However, cell-based therapy for clinical application has been hindered by limitations such as safety, immunogenicity issues, and difficulties in cell preservation, transportation, and phenotype control. In the current study, we aimed to produce a cell-free extract directly from human fat tissue and evaluate its potential therapeutic efficacy.MethodsWe developed a novel physical approach to produce a cell-free aqueous extract from human fat tissue (fat extract (FE)). The therapeutic potential of FE was investigated in the ischemic hindlimb model of nude mice. After establishment of hindlimb ischemia with ligation of the left femoral artery and intramuscular injection of FE, blood perfusion was monitored at days 0, 7, 14, 21, and 28. Tissue necrosis and capillary density were evaluated. Enzyme-linked immunosorbent assay was used to analyze the growth factors contained in FE. Moreover, the proliferation, migration, and tube formation ability were tested on human umbilical vein endothelial cells (HUVECs) in vitro when treated with FE. The proangiogenic ability of FE was further assessed in an in-vivo Matrigel plug assay.ResultsFE was prepared and characterized. The intramuscular injection of FE into the ischemic hindlimb of mice attenuated severe limb loss and increased blood flow and capillary density of the ischemic tissue. Enzyme-linked immunosorbent assay showed that FE contained high levels of various growth factors. When added as a cell culture supplement, FE promoted HUVEC proliferation, migration, and tube formation ability in a dose-dependent manner. The subcutaneous injection of Matrigel infused with FE enhanced vascular formation.ConclusionsWe developed a novel cell-free therapeutic agent, FE, produced from human adipose tissue. FE was able to attenuate ischemic injury and stimulate angiogenesis in ischemic tissues. This study indicates that FE may represent a novel cell-free therapeutic agent in the treatment of ischemic disorders.

show abstract

“…After centrifugation, we found that the processed lipoaspirates were separated into four parts (from top to bottom: lipid portion, lipid-devoid adipose tissue, liquid portion, and cell/tissue debris). The lipid-devoid adipose tissue, namely, extracellular matrix/stromal vascular fraction gel (SVF-gel), was found to have therapeutic ability in inducing angiogenesis and adipogenesis in vivo [20][21][22][23][24]. Unfortunately, the study of the liquid portion acquired from these physically processed lipoaspirates has been ignored.…”

Section: Introductionmentioning

confidence: 99%

Human adipose liquid extract induces angiogenesis and adipogenesis: a novel cell-free therapeutic agent

Xia

Chen

et al. 2019

Stem Cell Res Ther

View full text Add to dashboard Cite

Background Taking advantage of cellular paracrine mechanisms, the secretome of adipose-derived stem cells (ADSCs) and adipose tissue has been demonstrated to induce tissue repair and regeneration in various ischemic and impaired conditions. However, these cell-based therapies have been hindered by issues, such as inherent safety and cost-efficiency for clinical applications. In this study, we prepared a liquid cell-free extract from human adipose tissue [adipose liquid extract (ALE)] and evaluated its potential therapeutic efficacy. Methods ALE was prepared from human subcutaneous adipose tissue using a rapid and physical approach, and the protein components in ALE were identified using mass spectrometry analysis. In vivo, the therapeutic effect of this agent was investigated on wound healing in C57BL/6 mice, and wound healing rate, vessel density, and neo-adipocyte formation in wounded skins were measured at days 3, 7, 11, and 14. In vitro, the effect of ALE on the viability of human ADSCs, tube formation of human umbilical vein endothelial cells (HUVECs), and adipogenic differentiation of ADSCs were tested. Results The results demonstrated that ALE contained a variety of growth factors and did not affect cell viability. ALE-treated wounds exhibited accelerated wound healing with increased vessel density and formation of neo-adipocytes compared to that of control wounds. Moreover, when added as a cell culture supplement, ALE effectively induced tube formation of HUVECs and lipid accumulation in ADSCs. ALE-treated ADSCs also exhibited elevated levels of adipogenic gene expression. Conclusions ALE is a novel growth-rich therapeutic agent that is cell-free and easy to produce. Besides, it is also able to induce angiogenesis and adipogenesis both in vitro and in vivo, thus indicating that it could be used for wound repair and soft tissue regeneration.

show abstract

Ischemic flap survival improvement by composition-selective fat grafting with novel adipose tissue derived product - stromal vascular fraction gel

Cited by 30 publications

References 27 publications

Autologous Fractionated Adipose Tissue as a Natural Biomaterial and Novel One-Step Stem Cell Therapy for Repairing Articular Cartilage Defects

Autologous Fractionated Adipose Tissue as a Natural Biomaterial and Novel One-Step Stem Cell Therapy for Repairing Articular Cartilage Defects

Fat extract promotes angiogenesis in a murine model of limb ischemia: a novel cell-free therapeutic strategy

Human adipose liquid extract induces angiogenesis and adipogenesis: a novel cell-free therapeutic agent

Contact Info

Product

Resources

About