Ischaemic preconditioning protects the rat kidney from reperfusion injury

Abstract

Objective To examine the possible role of ischaemic at 0, 2 and 9 days after treatment, and expressed as a percentage of the total renal uptake. preconditioning (IPC), an adaptive pathophysiological phenomenon that increases tolerance to ischaemiaResults The mean (sem) maximum decrease in left renal function, to 14.5 (4.3)% of the total, occurred on day reperfusion (I-R) injury, in renal protection when rats are presented with an I-R challenge.2 in Group C. The equivalent value in Group D showed relative preservation of function, at 36.0 (3.5)% (P= Materials and methods Female Wistar rats (n=36) were divided randomly into four groups: (A) sham-operated 0.001 compared with Group C). The mean left renal function improved by day 9, to 39.6 (6.7)% (Group controls; (B) IPC only; (C) renal ischaemia (RI) only; and (D) IPC+RI. The left kidney in groups B and D C) and 48.6 (1.5)% (Group D). The mean left renal function in Group B (50.5-53.9%) did not diCer from was preconditioned with four cycles of renal artery occlusion lasting 4 min, each occlusion separated by that in controls (49.4-51.4%). Conclusion An IPC regimen applied 5 min before RI in 11 min of reperfusion. The ischaemic insult, applied in groups C and D, comprised 40 min of sustained left the rat significantly protects it from the functional impairment associated with ischaemia and reperrenal artery occlusion. In Group D, the IPC cycle was completed 5 min before the start of the ischaemic fusion.

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“…6,7 In the present study, the 48-hour reperfusion period led to clear histologic and molecular alterations, which are in line with previous studies. 1,4,26 Our finding that IPC does not alter histologic or molecular markers of renal injury in this model should be placed in the context of the current controversy as to whether IPC protects from subsequent renal IRI. Although several investigators have found IPC to be effective in protecting against renal IRI in various animal species, 3,[27][28][29][30] other studies have found no evidence that IPC is protective against renal injury.…”

Effect of ischemia preconditioning on renal ischemia/reperfusion injury in rats

“…before exposure to a 40 min. period of RI in the rat signifi cantly protected it from the functional impairment associated with kidney I/R (22). Furthermore, the results of an IP regimen (three 2 min.…”

No Protection of the Porcine Kidney by Ischaemic Preconditioning

“…If the interval between the last preconditioning ischaemia and the sustained ischaemia is too long the protective effect by classical IP is lost. Investigations on the effect of IP in rat kidneys demonstrated that an interval of 41 min resulted in loss of protection (Islam et al 1997), whereas intervals of 10 (Riera et al 1999) to 16 min (Toosy et al 1999;Jefayri et al 2000) did not alter the protective effect of IP in rat kidneys. The duration and severity of renal blood flow reduction predict the extent of the resulting functional and morphological damage (Moran et al 1992).…”

“…Therefore, this study related to SWOP rather than to classical IP. Recently published in vivo studies in rat kidneys reported attenuation of functional and morphological injury also by classical IP (Cochrane et al 1999;Riera et al 1999;Toosy et al 1999;Chien et al 1999; Lee & Emala, 2000;Jefayri et al 2000). In contrast to rat kidneys, IP did not protect human embryonic kidney cells in vitro, whereas rabbit ventricular myocytes and differentiated CµC12 myotubes were protected by IP (Liu et al 1996).…”