Isch�mie/Reperfusionsschaden der Leber durch freie radikale ? direkter radikalnachweis mittels elektronenspinresonanz (ESR)

Kunz, R.; Brune, Hans Albert; Ziegler, U.; Marzinzig, M.; Beger, H. G.

doi:10.1007/bf00250337

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…The ESR spectrum of guinea pig cochleas had three main peaks, peak I (e ES II((e E and III (d E [ Figure 2 ]. Peak I was a background peak of resonance, which appeared in all of the specimens for reasons that are unclear, not only in the cochleas,[ 29 ] but also in all of the other tissues[ 30 ] of animals without peaks found in this site. Peak II was called peak O ∥ , peak III was called peak O ⊥ .…”

Section: R Esultsmentioning

confidence: 99%

Solid Lipid Nanoparticles Loaded with Edaravone for Inner Ear Protection After Noise Exposure

Gao

Liu²,

Zhou³

et al. 2015

Chinese Medical Journal

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Background:Antioxidants and the duration of treatment after noise exposure on hearing recovery are important. We investigated the protective effects of an antioxidant substance, edaravone, and its slow-release dosage form, edaravone solid lipid nanoparticles (SLNs), in steady noise-exposed guinea pigs.Methods:SLNs loaded with edaravone were produced by an ultrasound technique. Edaravone solution or edaravone SLNs were administered by intratympanic or intravenous injection after the 1st day of noise exposure. Guinea pigs were exposed to 110 dB sound pressure level (SPL) noise, centered at 0.25–4.0 kHz, for 4 days at 2 h/d. After noise exposure, the guinea pigs underwent auditory brainstem response (ABR) threshold measurements, reactive oxygen species (ROS) were detected in their cochleas with electron spin resonance (ESR), and outer hair cells (OHCs) were counted with silvernitrate (AgNO3) staining at 1, 4, and 6 days.Results:The ultrasound technique was able to prepare adequate edaravone SLNs with a mean particle size of 93.6 nm and entrapment efficiency of 76.7%. Acoustic stress-induced ROS formation and edaravone exerted a protective effect on the cochlea. Comparisons of hearing thresholds and ROS changes in different animal groups showed that the threshold shift and ROS generation were significantly lower in treated animals than in those without treatment, especially in the edaravone SLN intratympanic injection group.Conclusions:Edaravone SLNs show noticeable slow-release effects and have certain protective effects against noise-induced hearing loss (NIHL).

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Section: R Esultsmentioning

confidence: 99%

Solid Lipid Nanoparticles Loaded with Edaravone for Inner Ear Protection After Noise Exposure

Gao

Liu²,

Zhou³

et al. 2015

Chinese Medical Journal

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The surgical school of Hans G. Beger in Ulm

Bittner

Büchler

2007

The American Journal of Surgery

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Hepatobiliary scintigraphy for evaluating the hepatotoxic effect of halothane and the protective effect of catechin in comparison with histo-chemical analysis of liver tissue

Karamanlıoğlu

Yüksel²,

Temiz³

et al. 2002

Nuclear Medicine Communications

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Halothane and its metabolites cause liver damage by decreasing liver blood flow and generating free-radical species. Catechin suppresses lipid peroxidation and increases enzyme activity, therefore it seems to be capable of protecting liver parenchyma against the direct toxic effect of halothane. The aim of this study was to investigate the role of hepatobiliary scintigraphy in detecting liver damage after halothane anaesthesia and the protective effect of catechin in comparison with histo-chemical analysis. Thirty rabbits, divided into three groups (A, controls; B, halothane; and C, catechin+halothane), were investigated. In group A no anaesthesia was administered. Group B only received halothane, while group C was pretreated with catechin and halothane anaesthesia was administered for 2 h. Dynamic scintigrams were taken for 60 min after injecting 99mTc-mebrofenin, and the time of peak uptake (TPU) and the time for half of the activity to clear from the liver (T(1/2)) were calculated. Rabbits were killed, and malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) levels were measured in hepatic tissue. The TPU and T(1/2) values of group A is significantly lower than in groups B and C (P<0.0002 and P<0.0002, respectively, for TPU; and P<0.0002 and P<0.0003, respectively, for T(1/2)). The TPU and T(1/2) values of group B were significantly higher than in group C (P<0.0003 and P<0.0003, respectively). The hepatic MDA level of group A was significantly lower than in groups B and C (P<0.0002 and P<0.0002, respectively). SOD, GSH-Px and CAT levels of group A were significantly higher than in groups B and C (P<0.0002, P<0.0001 and P<0.003, respectively, for group A vs group B; and P<0.0005, P<0.0002 and P<0.03, respectively, for group A vs group C). The MDA level of group B was significantly higher than that in group C (P<0.0002). SOD, GSH-Px and CAT levels of group B were significantly lower than in group C (P<0.0002, P<0.0002 and P<0.003, respectively). According to these results, we suggest that catechin protects liver parenchyma against the toxic effect of halothane and its metabolites, and that, compared to invasive histo-chemical analysis, hepatobiliary scintigraphy is a useful and alternative non-invasive method for detecting the protective effect of catechin on liver parenchyma after halothane anaesthesia.

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Isch�mie/Reperfusionsschaden der Leber durch freie radikale ? direkter radikalnachweis mittels elektronenspinresonanz (ESR)

Cited by 3 publications

References 24 publications

Solid Lipid Nanoparticles Loaded with Edaravone for Inner Ear Protection After Noise Exposure

Solid Lipid Nanoparticles Loaded with Edaravone for Inner Ear Protection After Noise Exposure

The surgical school of Hans G. Beger in Ulm

Hepatobiliary scintigraphy for evaluating the hepatotoxic effect of halothane and the protective effect of catechin in comparison with histo-chemical analysis of liver tissue

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