ISAba36 inserted into the outer membrane protein gene carO and associated with the carbapenemase gene blaOXA-24-like in Acinetobacter baumannii

Khorsi, Khadidja; Messai, Yamina; Ammari, Houria; Hamidi, Moufida; Bakour, Rabah

doi:10.1016/j.jgar.2018.08.020

Cited by 6 publications

(5 citation statements)

References 8 publications

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“…They have demonstrated that CarO serves as an uptake channel for L-ornithine and possibly carbapenems [66]. In carbapenem-resistant clinical isolates of A. baumannii insertion, elements can disrupt the expression of the chromosomal CarO gene and lead to complete loss of CarO [65,68,69]. In addition, CarO gene mutations can induce significant structural modifications in CarO and thus reduce outer membrane permeability and eventually cause drug resistance [70].…”

Section: Outer Membrane Proteinsmentioning

confidence: 99%

Acinetobacter baumannii Antibiotic Resistance Mechanisms

et al. 2021

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Acinetobacter baumannii is a Gram-negative ESKAPE microorganism that poses a threat to public health by causing severe and invasive (mostly nosocomial) infections linked with high mortality rates. During the last years, this pathogen displayed multidrug resistance (MDR), mainly due to extensive antibiotic abuse and poor stewardship. MDR isolates are associated with medical history of long hospitalization stays, presence of catheters, and mechanical ventilation, while immunocompromised and severely ill hosts predispose to invasive infections. Next-generation sequencing techniques have revolutionized diagnosis of severe A. baumannii infections, contributing to timely diagnosis and personalized therapeutic regimens according to the identification of the respective resistance genes. The aim of this review is to describe in detail all current knowledge on the genetic background of A. baumannii resistance mechanisms in humans as regards beta-lactams (penicillins, cephalosporins, carbapenems, monobactams, and beta-lactamase inhibitors), aminoglycosides, tetracyclines, fluoroquinolones, macrolides, lincosamides, streptogramin antibiotics, polymyxins, and others (amphenicols, oxazolidinones, rifamycins, fosfomycin, diaminopyrimidines, sulfonamides, glycopeptide, and lipopeptide antibiotics). Mechanisms of antimicrobial resistance refer mainly to regulation of antibiotic transportation through bacterial membranes, alteration of the antibiotic target site, and enzymatic modifications resulting in antibiotic neutralization. Virulence factors that may affect antibiotic susceptibility profiles and confer drug resistance are also being discussed. Reports from cases of A. baumannii coinfection with SARS-CoV-2 during the COVID-19 pandemic in terms of resistance profiles and MDR genes have been investigated.

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Section: Outer Membrane Proteinsmentioning

confidence: 99%

Acinetobacter baumannii Antibiotic Resistance Mechanisms

et al. 2021

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“…Besides nutrient availability, macromolecular secretions, pili expression, environmental signals, the heat-modifiable protein OmpA, and biofilm-related protein BamA all together have a significant role in modulating biofilm formation and adhesion in A. baumannii ( Singh et al, 2014 , 2017 ; Lin et al, 2015 ; Nie et al, 2020 ). The virulence factors exposed on cell-envelope include Omp33–36, Omp22, CarO, OmpW, LptD, and OprD, which protect cells against physiological response, alter cell envelope integrity to being assailed by harsh environmental conditions, as well as exposure to treatment options, such as cephalosporins and carbapenems ( Catel-Ferreira et al, 2012 , 2016 ; Bojkovic et al, 2015 ; Abellón-Ruiz et al, 2018 ; Guo et al, 2018 ; Khorsi et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Genotypic and Phenotypic Characterization of Novel Sequence Types of Carbapenem-Resistant Acinetobacter baumannii, With Heterogeneous Resistance Determinants and Targeted Variations in Efflux Operons

2021

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Acinetobacter baumannii has emerged as one of the dominant nosocomial human pathogens associated with high morbidity and mortality globally. Increased incidences of carbapenem-resistant A. baumannii (CRAB) have resulted in an enormous socioeconomic burden on health-care systems. Here, we report the genotypic and phenotypic characterization of novel ST1816 and ST128 variants in A. baumannii strains belonging to International clone II (GC2) with capsule types KL1:OCL8 and KL3:OCL1d from India. Sequence analysis revealed the presence of diverse virulome and resistome in these clinical strains, in addition to islands, prophages, and resistance genes. The oxacillinase blaOXA–23detected in the genomic island also highlighted the coexistence of blaOXA–66/blaOXA–98, blaADC73/blaADC–3, and blaTEM–1D in their mobile scaffolds, which is alarming. Together with these resistance-determining enzymes, multidrug efflux transporters also harbored substitutions, with increased expression in CRAB strains. The hotspot mutations in colistin resistance-conferring operons, PmrAB, LpxACD, and AdeRS, were additionally confirmed. Phenotype microarray analysis indicated that multidrug-resistant strains A. baumannii DR2 and A. baumannii AB067 preferred a range of antimicrobial compounds as their substrates relative to the other. To our knowledge, this is the first comprehensive report on the characterization of A. baumannii variants ST1816 and ST128, with different genetic makeup and genome organization. The occurrence of CRAB infections worldwide is a severe threat to available limited therapeutic options; hence, continued surveillance to monitor the emergence and dissemination of such novel ST variants in A. baumannii is imperative.

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“…[10][11][12] Carbapenem-resistant Acinetobacter species are mainly associated with the carbapenem-hydrolyzing NDM and CHDLs, such as bla OXA-23 -like, bla OXA-24/40 -like, bla OXA-51 -like, bla OXA-134 -like and bla OXA-211 -like gene. [13][14][15][16][17][18] Especially, co-carrying of the bla NDM and bla OXA in clinical, food, environmental derived isolates of Acinetobacter species were prevalent in the world. 11,[19][20][21] In addition, the genes encoding bla NDM and bla OXA are known to be carried on some mobile genetic elements that inserted into the chromosome or plasmids, and it is suspected the mechanism of horizontal gene transfer (HGT) promotes the exchange of resistance genes among pathogenic microorganisms isolated from the clinical, environmental samples, and food animals.…”

Section: Introductionmentioning

confidence: 99%

Whole-Genome Analysis of Two Copies of blaNDM-1 Gene Carrying Acinetobacter johnsonii Strain Acsw19 Isolated from Sichuan, China

Tang

Shen

Zhang

et al. 2020

IDR

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Purpose: To characterize the genetic feature of the carbapenems resistant Acinetobacter johnsonii strain Acsw19 isolated from municipal sludge. This strain was found to carry two copies of bla NDM-1, cmlB1-like gene, and bla OXA-211-like gene along with other 8 antimicrobial resistance genes, 3 plasmids, 15 genomic islands and 8 prophages. Methods: A carbapenem-resistant Acinetobacter johnsonii strain Acsw19 isolated from municipal sludge was subjected to whole-genome sequencing (WGS) via the PacBio and Illumina MiSeq platforms. Thereafter, the characteristic was analyzed by a series of bioinformatics software. Results: The results showed that the genome of Acsw19 was consisted of a 3,433,749 bp circular chromosome and 3 circular plasmids, pAcsw19-1 (11,161 bp), pAcsw19-2 (351,885 bp) and pAcsw19-3 (38,391bp), respectively. Resistome analysis showed that Acsw19 carried 12 antimicrobial resistance genes, including 6 [cmlB1-like, bla NDM-1 , bla OXA-58 , aph (3ʹ)-VIa, msr(E) and mph(E)] in the plasmid pAcsw19-2 and 6 (bla OXA-211-like, bla NDM-1 , aph(3")-Ib, aph(6)-Id, sul2, and floR) in the chromosome genome. Specifically, the cmlB1-like gene shared 86.33%, 71.7% and 71.9% similarities with the cmlB1, cmlA4 and cmlA8 gene, and the bla OXA-211-like gene shared 94.4%, 95.39% and 96.36% similarities with bla OXA-211 , bla OXA-643 and bla OXA-652 , at the nucleotide level, respectively. Phylogenetic analysis showed that the bla OXA-211-like gene and cmlB1-like gene had the closest evolutionary relationship with bla OXA-643 and cmlB1, respectively. These results indicated that the bla OXA-211-like and cmlB1-like genes identified in the current study should be the novel variant resistance genes. Conclusion: Carrying of two copies of bla NDM-1 , cmlB1-like, bla OXA-211-like and along with other 8 antimicrobial resistance genes, 3 plasmids, 15 genomic islands and 8 prophages Acinetobacter johnsonii strain might increase the possibility of spreading of resistance genes.

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ISAba36 inserted into the outer membrane protein gene carO and associated with the carbapenemase gene blaOXA-24-like in Acinetobacter baumannii

Cited by 6 publications

References 8 publications

Acinetobacter baumannii Antibiotic Resistance Mechanisms

Acinetobacter baumannii Antibiotic Resistance Mechanisms

Genotypic and Phenotypic Characterization of Novel Sequence Types of Carbapenem-Resistant Acinetobacter baumannii, With Heterogeneous Resistance Determinants and Targeted Variations in Efflux Operons

<p>Whole-Genome Analysis of Two Copies of <em>bla</em><sub>NDM-1</sub> Gene Carrying <em>Acinetobacter johnsonii</em> Strain Acsw19 Isolated from Sichuan, China</p>

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