IS1111 insertion sequences of Coxiella burnetii: characterization and use for repetitive element PCR-based differentiation of Coxiella burnetii isolates

Denison, Amy M.; Thompson, Herbert A.; Massung, Robert F.

doi:10.1186/1471-2180-7-91

Cited by 61 publications

(45 citation statements)

References 18 publications

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“…The majority of PCR assays for C. burnetii have targeted the insertion sequence element IS1111 (6)(7)(8)(9)(10). As a mobile genetic element, there is the potential for IS1111 to move from one organism type to another, theoretically resulting in loss of specificity (11).…”

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A Case of Q Fever Prosthetic Joint Infection and Description of an Assay for Detection of Coxiella burnetii

et al. 2013

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We present the first published case of Coxiella burnetii prosthetic joint infection. Diagnosis was established with PCR and culture of periprosthetic tissue and synovial fluid (and serology). A novel PCR assay is described herein. Q fever should be considered in patients with prosthetic joint infection without an identified pathogen. J oint replacement is a life-enhancing procedure performed on hundreds of thousands of patients in the United States each year. While prosthetic joint infection (PJI) occurs in only approximately 2% of patients following knee arthroplasty (1), it is a devastating complication. Accurate microbiologic diagnosis of PJI is important for directing appropriate management. There is no identified pathogen in approximately 7% of PJI cases, frequently as a result of antecedent antimicrobial therapy, but occasionally due to an inability to detect a pathogen by standard laboratory methods (2). Coxiella burnetii is not an organism that would traditionally be considered in a case of culture-negative PJI. We describe here the first published case of PJI caused by C. burnetii.The causative agent of the zoonosis Q fever, C. burnetii, is a small, obligate intracellular Gram-negative bacterium. Acute and chronic types of Q fever are classically diagnosed based on compatible clinical illnesses, supported by serologic results (3). Isolation of C. burnetii in cell culture is laborious, requires specially trained staff, and poses a safety risk to staff (4, 5), confining C. burnetii culture to a small number of research and public health laboratories and limiting medical practitioners' access to this technique for diagnostics.Q fever PCR offers a safe alternative to culture for direct detection of C. burnetii in clinical specimens. The majority of PCR assays for C. burnetii have targeted the insertion sequence element IS1111 (6-10). As a mobile genetic element, there is the potential for IS1111 to move from one organism type to another, theoretically resulting in loss of specificity (11). The diagnosis of C. burnetii PJI in the case reported here was made using a novel real-time PCR assay (described below) targeting the single-copy housekeeping gene target, shikimate dehydrogenase (aroE) of C. burnetii. CASE REPORTA 56-year-old male presented to our institution with new increasing right knee pain and swelling around a previously revised total knee arthroplasty (TKA).He first developed right knee pain after patellectomy for a comminuted patellar fracture at 27 years of age. He underwent repeated arthroscopies without improvement; right TKA was performed at age 44. This was complicated by early postoperative deep vein thrombosis (DVT). He developed increasing right knee pain, swelling, and stiffness approximately 12 weeks after arthroplasty. Subcutaneous corticosteroid and lidocaine injections were administered around the branches of the saphenous nerve on a monthly basis for presumed neuropathic pain.At the age of 53, he presented to our facility with a 5-day history of general malaise, mild nonproductive c...

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A Case of Q Fever Prosthetic Joint Infection and Description of an Assay for Detection of Coxiella burnetii

et al. 2013

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“…Alternatively IS1111 transposons could be present but with highly variable sequences. Denison et al (2007) reported some sequence variability and deletions in individual copies of the IS1111 transposon of C. burnetii. Based on the data by Denison et al (2007) and GenBank sequences the primers described by Willems et al (1994) should amplify at least one copy of the IS1111 transposon in all C. burnetii isolates.…”

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confidence: 99%

Molecular evidence for a novel Coxiella from Argas monolakensis (Acari: Argasidae) from Mono Lake, California, USA

Reeves

2008

Exp Appl Acarol

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Argasid ticks are vectors of viral and bacterial agents of humans and animals. Recent reports indicate that some ornithophilic argasids harbored rickettsial agents. A Nearctic tick, Argas monolakensis Schwan, Corwin, Brown is ornithophilic and has not previously been examined for rickettsial agents. Thirty adult A. monolakensis were tested by PCR for DNA from Rickettsia or Coxiella. Amplicons from a Coxiella sp. that were divergent from Coxiella burnetii were detected in 16/30 A. monolakensis. These molecular isolates were similar but not identical to C. burnetii, the Coxiella spp. of other ticks, and "Coxiella cheraxi" a pathogen of crayfish.

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“…The three other isolates used in this study were from acute cases of Q fever in Australia and were classified as group III (typing method from [10] (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Growth Yields of Four <i>Coxiella burnetii</i> Isolates in Four Different Cell Culture Lines

Lockhart¹,

Islam²,

Fenwick³

et al. 2013

AiM

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Although Coxiella burnetii is considered to be an obligate intracellular bacterium and grows in embryonated eggs, laboratory animals and cell culture, recently it has been grown in cell-free media and on agar plates. This current study was conducted to compare four cell lines for their yield of C. burnetii. Four different isolates of C. burnetii (Henzerling, Arandale, Cumberland and Timony) were grown in DH82, L929, Vero and XTC-2 cell lines. The DH82 and XTC-2 cells lines produced the highest C. burnetii yield which was slightly less than the yields achieved in recently published studies using cell free media. The Arandale isolate of C. burnetii produced a significantly higher yield in DH82 cells compared to XTC-2 cells (P < 0.03).

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IS1111 insertion sequences of Coxiella burnetii: characterization and use for repetitive element PCR-based differentiation of Coxiella burnetii isolates

Cited by 61 publications

References 18 publications

A Case of Q Fever Prosthetic Joint Infection and Description of an Assay for Detection of Coxiella burnetii

A Case of Q Fever Prosthetic Joint Infection and Description of an Assay for Detection of Coxiella burnetii

Molecular evidence for a novel Coxiella from Argas monolakensis (Acari: Argasidae) from Mono Lake, California, USA

Growth Yields of Four <i>Coxiella burnetii</i> Isolates in Four Different Cell Culture Lines

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IS1111 insertion sequences of Coxiella burnetii: characterization and use for repetitive element PCR-based differentiation of Coxiella burnetii isolates

Cited by 61 publications

References 18 publications

A Case of Q Fever Prosthetic Joint Infection and Description of an Assay for Detection of Coxiella burnetii

A Case of Q Fever Prosthetic Joint Infection and Description of an Assay for Detection of Coxiella burnetii

Molecular evidence for a novel Coxiella from Argas monolakensis (Acari: Argasidae) from Mono Lake, California, USA

Growth Yields of Four &lt;i&gt;Coxiella burnetii&lt;/i&gt; Isolates in Four Different Cell Culture Lines

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Growth Yields of Four <i>Coxiella burnetii</i> Isolates in Four Different Cell Culture Lines