A stable live avirulent, genetically modified Acya Acrp SalmoneUla typhimurium vaccine strain, x3985, was used in several vaccination strategies to evaluate its use in the control of SalmoneUla infection in chickens. Oral vaccination of chickens at 1 and at 14 days of age with 108 CFU of x3985 protected against invasion of spleen, ovary, and bursa of Fabricius and colonization of the ileum and cecum in chickens challenged with 106 CFU of virulent homologous SalmoneUla strains from group B. Chickens challenged with heterologous Salmonella strains from groups C, D, and E were protected against visceral invasion of spleen and ovary, while invasion of the bursa of Fabricius and colonization of ileum and cecum was reduced in vaccinated chickens. Oral vaccination at 2 and at 4 weeks of age induced an excellent protection against challenge with virulent group B Salmonella serotypes and very good protection against challenge with group D or E SalmoneUla serotypes, while protection against challenge with group C SalmoneUa serotypes was marginal but significant. Vaccination at 2 and at 4 weeks of age also protected vaccinated chickens against challenge with 108 CFU of highly invasive S. typhimurium or S. enteritidis strains. The protection of chickens vaccinated with X3985 against challenge with homologous and heterologous Salmonella serotypes is outstanding, and the complete protection against ovarian