Is there any correlation between apoptotic genes expression in cumulus cells with embryo morphokinetics?

Faramarzi, Azita; Khalili, Mohammad Ali; Jahromi, Masoumeh Golestan

doi:10.1007/s11033-019-04781-z

Cited by 9 publications

(5 citation statements)

References 52 publications

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“…Furthermore, the interactions between the oocyte and the CCs were revealed to significantly influence oocyte maturation rates and were found to be essential for generating embryos with high developmental competence (32). The association between CC apoptotic gene expression and embryo morphogenetics and cleavage pattern was also demonstrated (33). Therefore, CCs play an essential role in oocyte developmental competence and embryo viability in their early stage.…”

Section: Introductionmentioning

confidence: 99%

Resolvin E1 in Follicular Fluid Acts as a Potential Biomarker and Improves Oocyte Developmental Competence by Optimizing Cumulus Cells

Zhang

Zhu

et al. 2020

Front. Endocrinol.

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Metabolic profile of follicular fluid (FF) has been investigated to look for biomarkers for oocyte quality. Resolvin E1 (RvE1), a potent pro-resolving mediator, was reported to have protective action in cell function. The study aimed to examine the predictive value of RvE1 for oocyte quality and to explore the cellular mechanism of RvE1 in improving oocyte competence. Metabolic profiles of 80 FF samples showed a higher level of RvE1 in group A (blastocysts scored ≥ B3BC and B3CB according to Gardner's blastocyst scoring system, N = 36) than that of group B (blastocysts scored < B3BC and B3CB, N = 44, P = 0.0018). The receiver operating characteristic (ROC) curve analysis showed that RvE1 level in FF below 8.96 pg/ml (AUC:0.75; 95%CI: 0.64-0.86; P = 0.00012) could predict poor oocyte quality with specificity of 97.22%, suggesting RvE1 as a potential biomarker to exclude inferior oocytes. Besides, the level of RvE1 was found to be significantly lower in FF than in serum (57.49 to 17.62 pg/ml; P = 0.0037) and was gradually accumulated in the culture medium of cumulus cells (CCs) during cell culture, which indicated that RvE1 came from both blood exudates and local secretion. The in vitro experiment revealed the cellular mechanism of RvE1 in improving oocyte quality by decreasing the cumulus cell apoptotic rate and increasing cell viability and proliferation. It is the first time that the role of RvE1 in reproduction is explored. In conclusion, RvE1 is valuable as a potential exclusive biomarker for oocyte selection and plays a role in improving oocyte quality.

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Section: Introductionmentioning

confidence: 99%

Resolvin E1 in Follicular Fluid Acts as a Potential Biomarker and Improves Oocyte Developmental Competence by Optimizing Cumulus Cells

Zhang

Zhu

et al. 2020

Front. Endocrinol.

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“…Also, calculated morphokinetic variables were evaluated according to s1 (t2-tPNF), s2 (t4-t3), s3 (t8-t5), and duration of the second cell cycle (cc2a = t3-t2, cc2b = t4-t2) and third cell cycle (cc3a = t5-t4, cc3b = t6-t4, cc3c = t7-t4, cc3d = t8-t4). The rate of embryo arrest and cleavage anomalies was calculated, including uneven blastomere size at two-cell stage, multinucleation in blastomere at two-and four-cell stage, direct cleavage (DC), reverse cleavage (RC), and atypical phenotypes as presence of fragmentation and vacuoles [15].…”

Section: Ovarian Hyperstimulationmentioning

confidence: 99%

The Consequence of Short Insemination Strategy on Sperm Biological Characteristics, Embryo Morphokinetics, and Clinical Outcomes in the IVF Program

Anbari,

Khalili,

Mahaldashtian

et al. 2023

Andrologia

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Overnight coincubation of the cumulus–oocyte complexes (COCs) with spermatozoa may be accompanied by overproduction of reactive oxygen species (ROS) and possible determinant effects on sperm cellular status in the in vitro fertilization (IVF) program. The COCs from 100 cases were divided into two groups of short (2 hr) and long (18 hr) inseminations. The malondialdehyde (MDA) level as by-product of ROS was assessed in the insemination medium. The sperm DNA integrity, the mitochondrial membrane potential (MMP), and acrosome reaction (AR) were evaluated using sperm chromatin dispersion (SCD), fluorescence stain of JC-1, and double staining, respectively. Normally, fertilized oocytes (n = 525) were assessed via time-lapse monitoring (TLM) for assessment of the time of fading (tPNF), 2 until 8 timing (t2-t8), s1 (t2-tPNF), s2 (t4-t3), s3 (t8-t5), and duration of cell cycles. Finally, the best embryos were transferred, and clinical outcomes were assessed. Higher rates of MDA concentration, DNA fragmentation, and AR and low rate of MMP were noticed in long compared to short insemination groups ( p ≤ 0.0001 ). The morphokinetic parameters showed five-cell stage (t5) and cell cycle 3 (cc3) that were significantly different between the groups ( p = 0.04 and p = 0.03 , respectively). A high level of ROS observed in the long insemination group might have a detrimental effect on sperm status. Although, similar embryo quality and clinical outcomes were noticed in two insemination groups, but the trend was toward the short insemination.

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“…Combination of morphological and morphokinetic parameters can assist in selecting the embryos with high implantation potential. Time‐laps monitoring (TLM) is a predicting tool for evaluating embryos with higher development potential, implantation and even embryo aneuploidy status (Faramarzi, Khalili, & Jahromi, 2019; Mangoli et al., 2019; Omidi, Khalili, Halvaei, Montazeri, & Kalantar, 2020). This technology allows investigation of embryo development in stable incubator condition (Bhide et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

Does sperm DNA fragmentation have negative impact on embryo morphology and morphokinetics in IVF programme?

et al. 2020

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Evaluation of sperm integrity may predict the in vitro fertilisation (IVF) outcomes. The aim was to evaluate the relationship between the sperm DNA fragmentation (sDNAf) with embryo morphology and morphokinetic using time‐laps monitoring (TLM) and to select the best time points for normalisation in IVF setting. After evaluating the fertilisation and pronuclei (Z) scoring, 328 normally fertilised oocytes were assessed to time of pronuclei fading, time of 2 to 8 discrete cells (t2–t8) and abnormal cleavage patterns, such as multinucleation, direct cleavage, reverse cleavage and fragmentation. Sperm chromatin dispersion (SCD) assay was used for assessment of prepared sperm chromatin status. SCD was categorised into 4 groups of <6.5, 6.5–10.7, 10.7–20.1 and >20.1. The finding showed significant differences in t6 (p = .012), t7 (p = .045), t8 (p = .013) and s1 (p = .001) between 4 SCD groups. When morphokinetic variables were normalised to tPNf, this difference was observed in t2 (p = .003) and t6 (p = .017). Subsequently, the percentage of top quality embryos and Z1 scoring were dependent to the sDNAf rate. In conclusion, tPNf was the best reference time point in IVF cycles. Also, we found high sDNAf rate had no negative impact on embryo morphology and morphokinetics in conventional IVF.

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Is there any correlation between apoptotic genes expression in cumulus cells with embryo morphokinetics?

Cited by 9 publications

References 52 publications

Resolvin E1 in Follicular Fluid Acts as a Potential Biomarker and Improves Oocyte Developmental Competence by Optimizing Cumulus Cells

Resolvin E1 in Follicular Fluid Acts as a Potential Biomarker and Improves Oocyte Developmental Competence by Optimizing Cumulus Cells

The Consequence of Short Insemination Strategy on Sperm Biological Characteristics, Embryo Morphokinetics, and Clinical Outcomes in the IVF Program

Does sperm DNA fragmentation have negative impact on embryo morphology and morphokinetics in IVF programme?

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