Is the fish embryo toxicity test (FET) with the zebrafish (Danio rerio) a potential alternative for the fish acute toxicity test?

Exposure to DE-71 alters thyroid hormone levels and gene transcription in the hypothalamic–pituitary–thyroid axis of zebrafish larvae

“…The results of the present study indicate that PBDE exposure modulates gene transcription in the HPT axis as well as affects the levels of THs in zebrafish larvae; therefore, zebrafish embryos/larvae can be considered as a reliable model for investigating developmental toxicity of PBDEs and mechanisms by which PBDEs disrupt the activity of THs. The new European Chemical Policy REACH (registration, evaluation, and authorization of chemicals) states that fish embryo toxicity tests can be an alternative approach for toxicity assessment (Lammer et al, 2009). In line with this view, the results of the present study also suggested that zebrafish embryos/larvae can represent a valuable and powerful alternative for novel studies of large-scale screening of chemicals that can disrupt the activity of thyroid hormones.…”

Waterborne exposure to PFOS causes disruption of the hypothalamus–pituitary–thyroid axis in zebrafish larvae

“…In addition to a high public demand for the reduction or replacement of animal tests for ethical and political reasons, there are also scientific and cost-effective reasons to embrace a more differentiated testing approach (Lammer et al, 2009). In this regard, a toxicity test in fish embryos provides an alternative approach for toxicity assessment (Scholz and Mayer, 2008;Lammer et al, 2009).…”

Image-Based Fluidic Sorting System for Automated Zebrafish Egg Sorting into Multiwell Plates

“…The other half were used in the ZebraFactor to automatically fill 96-well plates. We followed the procedure described by Lammer et al, 6 except that eggs were dispensed at approximately 4 h instead of 3 h postfertilization (hpf). The time for each run was measured and the errors noted.…”