Exposure to DE-71 alters thyroid hormone levels and gene transcription in the hypothalamic–pituitary–thyroid axis of zebrafish larvae

Effects of acute exposure to polybrominated diphenyl ethers on retinoid signaling in zebrafish larvae

“…Adult zebrafish (AB strain) maintenance and embryos exposure were carried out as detailed by Yu et al (2010). Embryos that developed normally and reached the blastula stage (2 h post-fertilization, hpf) were selected for further experimentation.…”

“…Gene transcription analyses were performed as described previously by Yu et al (2010). Briefly, total RNA was extracted from 30 homogenized larvae exposed to each treatment using Trizol Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.…”

Acute exposure to DE‐71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae

“…Adult zebrafish (Danio rerio; AB strain) maintenance and embryo exposure were carried out as described by Yu et al [26]. Zebrafish embryos that had developed normally and reached blastula stage (2 h postfertilization; hpf) were selected for use in experiments.…”

“…At 120 hpf, 30 larvae from each beaker (n ¼ 3) were randomly sampled for total RNA extraction using Trizol reagent as described by Yu et al [26]. The detailed procedure for extraction, purification, and quantification of total RNA and first-strand cDNA synthesis was performed as described previously [26] and is given in the Supplemental Data (S4).…”

“…The detailed procedure for extraction, purification, and quantification of total RNA and first-strand cDNA synthesis was performed as described previously [26] and is given in the Supplemental Data (S4). The primer sequences of target genes were designed in Primer3 (http://frodo.wi.mit.edu/) and are shown in the Supplemental Data, Table S1.…”