Is propylene glycol an inert substance?

Hoenig, V; Werner, Françoise

doi:10.1016/0378-4274(80)90020-x

Cited by 5 publications

(1 citation statement)

References 14 publications

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“…Four‐ to six‐week‐old, male M. coucha , obtained from Mahatma Gandhi Institute of Medical Sciences, India, were immunized with 50 μg of purified recombinant NALT, LALT, NALT with DAG and LALT with DAG (five animals per group). The amount of dipalmitoyl glycerol in 50 μg of LALT was calculated to be 2 μg; hence, 1 mg/mL concentration of dipalmitoyl glycerol (Sigma Aldrich Chemicals, St. Louis, MO, USA) in propylene glycol was prepared as stock and 2 μL of it is mixed with 48 μg corresponding antigen for administering in Protein + DAG groups. Mastomys were handled in accordance with the institutional guidelines, and the experimental protocol was approved by the responsible institutional animal care committee.…”

Section: Methodsmentioning

confidence: 99%

Bacterial lipid modification enhances immunoprophylaxis of filarial abundant larval transcript‐2 protein in Mastomys model

Sharmila

Christiana

Pote

et al. 2013

Parasite Immunology

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As in many other parasitic diseases, efficacious vaccine for lymphatic filariasis has been elusive for want of new approaches leaving billions of people either debilitated or at risk. With multiple B- and T-cell epitopes, the abundant larval transcript-2 (ALT-2) of the filarial worm, Brugia malayi, has been shown to be a promising immunoprophylactic target. To enhance its efficacy, it was lipid modified using our recently developed protein engineering tool, which then offered 30% more immunoprotection (49 vs. 79%) in Mastomys coucha model. Sustained high levels of IFN-γ (about 100 times) and high antibody titres (10-fold) elicited by lipid-modified ALT-2, as compared to the native form, indicated the maintenance of Th1/Th2 balance that is impaired in filariasis. Thus, this study provides the basis for developing efficacious vaccines for filariasis and other parasitic diseases by exploiting bacterial lipid modification.

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Section: Methodsmentioning

confidence: 99%