Is pro-matrix metalloproteinase-3 a marker for posttraumatic cartilage degradation?

Bobacz, Klaus; Maier, R.; Fialka, Christian; Ekhart, H; Woloszczuk, W.; Geyer, G; Erlacher, L; Smolen, JS; Graninger, Winfried

doi:10.1016/s1063-4584(03)00159-6

Cited by 13 publications

(8 citation statements)

References 36 publications

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“…COMP is an extracellular matrix protein that is a biomarker for articular cartilage injury. The levels of COMP and MMP-3 increase in the serum of OA patients (Bobacz et al, 2003). COMP was originally found in articular cartilage, and later found in tendons, synovial membranes, and bones.…”

Section: Discussionmentioning

confidence: 95%

Cartilage oligomeric matrix protein and matrix metalloproteinase-3 expression in the serum and joint fluid of a reversible osteoarthritis rabbit model

Xq¹,

Wang²,

Ld³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The main pathological characteristic of osteoarthritis (OA) is cartilage damage. We explored cartilage oligomeric matrix protein (COMP) and matrix metalloproteinase-3 (MMP-3) changes during articular cartilage injury and repair. Rabbits were randomly divided into the following: a blank control group; groups M1, M2, and M3, in which breaking was performed for 2, 4, and 6 weeks, respectively; and groups L1, L2, and L3, in which breaking was discontinued for 2, 4, and 6 weeks, respectively, following a 4-week recovery period. There are 7 rabbits in each group. The degree of cartilage damage in each group was scored (OA score). An enzyme-linked immunosorbent assay was used to detect COMP and MMP-3 levels in serum and joint fluid. The OA scores were 3.89 ± 2.31, 7.21 ± 2.31, and 10.88 ± 2.08 points in groups M1, M2, and M3, respectively (P < 0.05). COMP and MMP-3 levels were significantly 14208 X.Q. Chu et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 14207-14215 (2015) higher in groups M1, M2, and M3 than in C. The OA score improved significantly following the 4-week recovery period (P < 0.05). COMP and MMP-3 levels began to decrease as the time following discontinuation of breaking increased, but were higher than in the control (P < 0.05). MMP-3 and COMP levels were correlated with OA score (r > 0.7, P < 0.05). COMP and MMP-3 levels were correlated between joint fluid and serum (r = 0.899, r = 0.874, P < 0.05, respectively). Long-term joint breaking can cause articular cartilage damage. Doing some activites after the process can promote self-repair of articular cartilage. COMP and MMP-3 levels were associated with articular cartilage destruction and repair.

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Section: Discussionmentioning

confidence: 95%

Cartilage oligomeric matrix protein and matrix metalloproteinase-3 expression in the serum and joint fluid of a reversible osteoarthritis rabbit model

Xq¹,

Wang²,

Ld³

et al. 2015

Genet. Mol. Res.

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“…MMP-13 has been shown to be five times to 10 times more active in digesting Type II collagen than other collagenases, and MMP-13 overexpression in mice has been associated with advanced joint degeneration [3,17,25,31]. Prior human studies have demonstrated elevated MMP concentrations in arthritic joint synovial fluid [4,21,23,28,34]. However, there were no prior studies evaluating for the presence of MMPs after articular fracture, which is the clinical setting with the most articular damage and the highest rates of posttraumatic OA.…”

Section: Discussionmentioning

confidence: 99%

“…A variety of animal OA models with ACL transection have shown upregulation of genes coding for aggrecanases and MMPs as well as increased concentrations of these destructive proteases [1,10,20,47]. Most human studies demonstrate elevated MMPs and aggrecan degradation in patients with OA as compared with healthy control subjects and that these proteases are more elevated in patients with end-stage OA compared with early OA [4,21,23,28,34]. In an effort to look specifically at the presence of proteolytic enzymes after traumatic injury, several authors have shown elevated MMPs and aggrecan degradation after ACL tear.…”

Section: Introductionmentioning

confidence: 99%

Intraarticular Matrix Metalloproteinases and Aggrecan Degradation Are Elevated After Articular Fracture

Haller

Swearingen

Partridge

et al. 2015

Clin Orthop Relat Res

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Background Posttraumatic osteoarthritis (OA) is a variant of OA that can develop after articular injury. Although the mechanism(s) of posttraumatic OA are uncertain, the presence and impact of postinjury proteolytic enzymes on articular cartilage remain unknown. To our knowledge, there are no studies that evaluate the presence of matrix metalloproteinases (MMPs) or aggrecan degradation after articular fracture.Questions/purposes (1) Are MMP concentrations and aggrecan degradation elevated after intraarticular fracture? (2) Are MMP concentrations and aggrecan degradation greater in high-energy injuries compared with low-energy injuries? (3) Do the concentrations of these biomarkers remain elevated at a secondary aspiration? Methods Between December 2011 and June 2013, we prospectively enrolled patients older than 18 years of age with acute tibial plateau fracture. Exclusion criteria included age older than 60 years, preexisting knee OA, injury greater than 24 hours before evaluation, contralateral knee injury, history of autoimmune disease, open fracture, and non-English-speaking patients. During the enrollment period, we enrolled 45 of the 91 (49%) tibial plateau fractures treated at our facility. Knee synovial fluid aspirations were obtained from both the injured and uninjured knees; two patients received aspirations in the emergency department and the remaining patients received aspirations in the operating room. Twenty patients who underwent spanning external fixator followed by definitive fixation were aspirated during both surgical procedures. MMP-1, -2, -3, -7, -9, -10, -12, and -13 concentrations were quantified using multiplex assays. Aggrecan degradation was quantified using sandwich enzyme-linked immunosorbent assay. Results There were higher concentrations of MMP-1 (3.89 ng/mL [95% confidence interval {CI}, 2.37-6.37] versus 0.37 ng/mL [95% CI, 0.23-0.61], p \ 0.001), MMP-3The institution of one or more of the authors (JMH, TFH) has received peer-reviewed research grant funding from the Orthopedic Trauma Association, LS Peery Foundation, and AO North America for this project. One of the authors certifies that he (TFH), or a member of his immediate family, has signed an agreement with DePuy Synthes (Warsaw, IN, USA) for consulting activities not related to the current study, and has not received payments or benefits, during the study period. One or more of the authors (CAS, KT) are employed at Eli Lilly (Indianapolis, IN, USA). All ICMJE Conflict of Interest Forms for authors and Clinical Orthopaedics and Related Research1 editors and board members are on file with the publication and can be viewed on request. Clinical Orthopaedics and Related Research1 neither advocates nor endorses the use of any treatment, drug, or device. Readers are encouraged to always seek additional information, including FDAapproval status, of any drug or device prior to clinical use. Each author certifies that his or her institution approved or waived approval for the reporting of this investigation and that all inves...

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“…Some investigators have failed to find a relationship between blood levels of MMP-3 and radiographic OA or between MMP-3 levels and markers of systemic inflammation or clinical features of OA, such as joint pain or functional impairment (8,9). Bobacz et al (28) found that serum levels of pro-MMP-3 were not related to the severity of cartilage damage in patients with knee injury who underwent arthroscopy because of persistent pain or joint instability. However, only 26% of the samples analyzed had a serum pro-MMP-3 level higher than normal, and levels of pro-MMP-3 did not correlate significantly with concentrations of highly sensitive C-reactive protein (CRP), which may reflect an inflammatory reaction within the joint.…”

Section: Lohmander Et Almentioning

confidence: 99%

Use of the plasma stromelysin (matrix metalloproteinase 3) concentration to predict joint space narrowing in knee osteoarthritis

Lohmander

Brandt

Mazzuca

et al. 2005

Arthritis & Rheumatism

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Is pro-matrix metalloproteinase-3 a marker for posttraumatic cartilage degradation?

Abstract: The measurement of proMMP-3 in serum or SF did not reflect the present cartilage damage and thus appears to have only minor potential for clinical use, but it should be considered for longitudinal studies, since it may reflect a risk for cartilage degradation in a subset of patients.

Cited by 13 publications

References 36 publications

Cartilage oligomeric matrix protein and matrix metalloproteinase-3 expression in the serum and joint fluid of a reversible osteoarthritis rabbit model

Cartilage oligomeric matrix protein and matrix metalloproteinase-3 expression in the serum and joint fluid of a reversible osteoarthritis rabbit model

Intraarticular Matrix Metalloproteinases and Aggrecan Degradation Are Elevated After Articular Fracture

Use of the plasma stromelysin (matrix metalloproteinase 3) concentration to predict joint space narrowing in knee osteoarthritis

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