Cartilage oligomeric matrix protein and matrix metalloproteinase-3 expression in the serum and joint fluid of a reversible osteoarthritis rabbit model

Abstract

ABSTRACT. The main pathological characteristic of osteoarthritis (OA) is cartilage damage. We explored cartilage oligomeric matrix protein (COMP) and matrix metalloproteinase-3 (MMP-3) changes during articular cartilage injury and repair. Rabbits were randomly divided into the following: a blank control group; groups M1, M2, and M3, in which breaking was performed for 2, 4, and 6 weeks, respectively; and groups L1, L2, and L3, in which breaking was discontinued for 2, 4, and 6 weeks, respectively, following a 4-week recovery period. There are 7 rabbits in each group. The degree of cartilage damage in each group was scored (OA score). An enzyme-linked immunosorbent assay was used to detect COMP and MMP-3 levels in serum and joint fluid. The OA scores were 3.89 ± 2.31, 7.21 ± 2.31, and 10.88 ± 2.08 points in groups M1, M2, and M3, respectively (P < 0.05). COMP and MMP-3 levels were significantly 14208 X.Q. Chu et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 14207-14215 (2015) higher in groups M1, M2, and M3 than in C. The OA score improved significantly following the 4-week recovery period (P < 0.05). COMP and MMP-3 levels began to decrease as the time following discontinuation of breaking increased, but were higher than in the control (P < 0.05). MMP-3 and COMP levels were correlated with OA score (r > 0.7, P < 0.05). COMP and MMP-3 levels were correlated between joint fluid and serum (r = 0.899, r = 0.874, P < 0.05, respectively). Long-term joint breaking can cause articular cartilage damage. Doing some activites after the process can promote self-repair of articular cartilage. COMP and MMP-3 levels were associated with articular cartilage destruction and repair.

Anti-Osteoarthritic Effects of a Mixture of Dried Pomegranate Concentrate Powder, Eucommiae Cortex, and Achyranthis Radix 5:4:1 (g/g) in a Surgically Induced Osteoarthritic Rabbit Model

“…Approximately 10 mL of whole blood was collected from the vena cava at euthanization, and the serum was separated by centrifugation at 3000 rpm for 10 min. Serum concentrations of COMP, CTX-II, and MMP-3 were measured using sandwich enzyme-linked immunosorbent assays (ELISAs) in accordance with previous reports [15,16]. A rabbit COMP ELISA kit (MBS721182, MyBioSource, San Diego, CA, USA), rabbit CTX-II ELISA kit (MBS705896, MyBioSource, San Diego, CA, USA) and rabbit MMP-3 ELISA kit (MBS2502146, MyBioSource, San Diego, CA, USA) were used in this measurement.…”

“…Kuhne et al [75] reported that the serum COMP level in serum increases in patients after traumatic knee injury. COMP may be instructive regarding cartilage degradation status, and has been used as a marker of OA [15,16,76]. This protein is a constituent of AC, but is also synthesized by tendon synoviocytes and fibroblasts [76,77].…”

“…Synovial fluid was collected from the operated knee before euthanization and centrifuged at 12,500 rpm for 10 min at 4 • C. Synovial concentrations of COMP, CTX-II, MMP-3, IL-1β, and TNF-α were measured using ELISAs in accordance with previous reports [15,16,49,58], using methods similar to those described for the serum measurements. A rabbit COMP ELISA kit (MBS721182, MyBioSource, San Diego, CA, USA), rabbit CTX-II ELISA kit (MBS705896, MyBioSource, San Diego, CA, USA), rabbit MMP-3 ELISA kit (MBS2502146, MyBioSource, San Diego, CA, USA), rabbit IL-1β ELISA kit (LS-F23299, LSBio Seattle, WA, USA) and rabbit TNF-α ELISA kit (MBS2500169, MyBioSource, San Diego, CA, USA) were used.…”

“…During tissue turnover, molecular biomarkers of OA are derived from tissue matrices released into fluids (i.e., blood, urine, or synovial fluid) [15,16]. They reflect bone or cartilage turnover and synovial metabolism, and can be used as an assessment of pathophysiological processes [17,18].…”

“…MMP-3 levels have been also associated with the loss of volume [21]. Therefore, the value of CTX-II, COMP, and MMP-3 has been used as biomarkers of early-stage OA [15,16,22].…”