Is photometry an accurate and reliable method to assess boar semen concentration?

Camus, Agnès; Camugli, S.; Lévêque, Christophe; Schmitt, Éric; Staub, Christophe

doi:10.1016/j.theriogenology.2010.09.025

Cited by 16 publications

(15 citation statements)

References 13 publications

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“…The accuracy of these systems depends not only on the optical properties and the software settings, but also on the kind of counting chamber that is used (Kuster, 2005). Nucleocounters are also used for determining sperm concentration, and they provide similar counts as those obtained with photometers (Camus et al, 2011;Hànsen and Hedeboe, 2003). In these devices, DNA is fluorescently labelled and counted by image analysis resulting in an accurate determination of sperm concentration.…”

Section: Other Methodsmentioning

confidence: 99%

Artificial Insemination in Pigs

Maes¹,

Alfonso²,

Rijsselaere³

et al. 2011

Artificial Insemination in Farm Animals

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Section: Other Methodsmentioning

confidence: 99%

Artificial Insemination in Pigs

Maes¹,

Alfonso²,

Rijsselaere³

et al. 2011

Artificial Insemination in Farm Animals

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“…Although considered the gold standard for humans, hemocytometer counts are time consuming and require adequate training in order to avoid variable results, which have been demonstrated by various studies ( WHO, 2010 ; Brito et al, 2016 ). Spectrophotometers, while precise, fast and relatively inexpensive, also have drawbacks of not being as accurate due to the inability to discriminate between spermatozoa and other cells, particles, or debris that may be in the suspension ( Anzar et al, 2009 ; Camus et al, 2011 ; Brito et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Technical Note: The use of iSperm technology for on-farm measurement of equine sperm motility and concentration

Moraes

Runcan

Blawut

et al. 2019

Translational Animal Science

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The iSperm is a newly released semen analysis tool from Aidmics Biotechnology Co. LTD, which allows an iPad Mini to be transformed into a handheld microscope with objective semen analysis software for equine available through the Apple Store (version 4.5.2). The aim of this study was to compare iSperm values for sperm motility and sperm concentration to current acceptable methods for semen analysis and to determine the agreement with these methods using statistical methods. Two ejaculates from each of five Standardbred stallions were used to compare sperm motility (computer-assisted semen analysis [CASA] vs. iSperm) and concentration (NucleoCounter SP-100 [NC] vs. hemocytometer vs. iSperm). Data were analyzed by first testing for the differences between the means of each method using a linear mixed-effects model. The agreement between the two continuous measurements for each method was then investigated by computing Lin’s concordance correlation coefficient (CCC), with a value of 1 indicating perfect agreement between methods. Results are reported as the CCC with the associated 95% confidence interval in parentheses. Means for both total motility (TM) and progressive motility (PM) were equal between CASA and iSperm values (P = 0.0741 and P = 0.725, respectively). However, means for all velocity measurements were significantly different between CASA and iSperm readings (P < 0.001). For concentration, means were equal between NC and iSperm values (P = 0.748) and for hemocytometer and iSperm values (P = 0.953). The CCC for TM was 0.871 (0.788, 0.923) and for PM was 0.916 (0.847, 0.955) indicating good agreement between methods. Low levels of agreement were observed for all velocity measurements. Finally, the CCC for concentration compared by iSperm and NC was 0.970 (0.949, 0.982) and for iSperm and hemocytometer it was 0.962 (0.934, 0.978), both close to the line of perfect concordance. Although more work is needed to improve the iSperm software for velocity measurements to be acceptable by research standards, in its present form the iSperm will introduce a low-cost and affordable method for on-farm semen analysis (TM, PM, concentration) for breeders and veterinarians. As a result, more farms will have access to accurate sperm analysis tools which will help to standardize semen processing procedures leading to better overall quality of semen used for artificial insemination.

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“…However, photometers are widely used routinely for determining sperm concentration by many AI organisations, for bulls and boars as well as other species (Woelders, 1991). They need to be evaluated before use, because accurate concentration measurement is the first and crucial step of the semen preparation process for production of semen doses (Camus et al, 2011). Correct assessment of sperm concentration is essential to ensure that the number of sperm per insemination dose meets requirements and that the maximal number of doses can be produced per ejaculate.…”

Section: Methods Agreement For Determining Sperm Concentrationmentioning

confidence: 99%

“…For example, the first large scale, nation-wide proficiency testing program for clinical andrology laboratories in the United States reported that the inter-laboratory coefficient of variation for manual sperm concentration determination was 80%, with a range for a single semen specimen of 3 -492 x 10 6 /ml (Keel et al, 2000). The accuracy, reliability and repeatability of different instruments that evaluate sperm concentration of raw semen have already been compared in several previous studies (Christensen et al, 2004;Hansen et al, 2006;Prathalingam et al, 2006;Anzar et al, 2009;Camus et al, 2011). Variation in the results from different laboratories could be due to the lack of standardisation of methods between laboratories (Maatson, 1995).…”

Section: Methods Agreement For Determining Sperm Concentrationmentioning

confidence: 99%

“…Animal species of agricultural interest are mainly produced by artificial insemination (AI) which contributes highly to the development of worldwide swine production, making the impact of the male in reproductive efficiency of the pig herds more crucial (Jounala et al, 1998). The efficiency of AI (fertility rate and prolificacy) is directly dependent on the quality of semen doses and on the number of spermatozoa used for insemination (Camus et al, 2011). In commercial farms, routine examination of boar semen is performed aiming to predict the male's fertility.…”

Section: Introductionmentioning

confidence: 99%

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