Artificial Insemination in Pigs

Maes, Dominiek; Alfonso, Lopez Rodriguez; Rijsselaere, Tom; Vyt, P; Ann, Van Soom

doi:10.5772/16592

Cited by 29 publications

(27 citation statements)

References 58 publications

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“…Average sperm production ranges between 60 and 120 billion sperm per ejaculate [39,42], depending on collection interval and boar age, and resulting in 20 to 40 traditional AI doses or 40 to 60 low sperm doses [20,33]. Collection most often occurs using the gloved-hand technique [43], but new equipment for semiautomation has been developed to aid boar collection [44,45]. Certain stud design features such as placement and use of warmup pens can help improve collection time and sperm output [42].…”

Section: Boar Stud Management For Production Of Semenmentioning

confidence: 99%

“…Certain stud design features such as placement and use of warmup pens can help improve collection time and sperm output [42]. Most AI centers have criteria for processing an ejaculate which include meeting standards for motility (70%) [40] and abnormalities (<20%) which may also include measures for total sperm, ejaculate volume, sperm concentration, agglutination, and bacterial contamination [8,43,46,47]. If the sample passes the minimum criteria, it is diluted with extender and then partitioned into disposable plastic containers that hold single AI doses of either 1.5 to 3.0 billion viable sperm in volumes that may range from 30 to 80 mL.…”

Section: Boar Stud Management For Production Of Semenmentioning

confidence: 99%

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Artificial insemination in pigs today

2016

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Section: Boar Stud Management For Production Of Semenmentioning

confidence: 99%

Section: Boar Stud Management For Production Of Semenmentioning

confidence: 99%

Artificial insemination in pigs today

2016

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“…The use of artificial insemination (AI) in commercial pig herds has increased significantly in the last decade [Maes et al 2011;Riesenbeck 2011]. To provide high quality insemination doses, routine assessment of the semen quality of the ejaculates is necessary.…”

Section: Introductionmentioning

confidence: 99%

Boar seminal plasma components and their relation with semen quality

Rodriguez

Rijsselaere

Beek

et al. 2012

Systems Biology in Reproductive Medicine

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Select boar seminal plasma (SP) components and their relation to semen quality were investigated. Thirty nine boars from three artificial insemination (AI) centers were divided into group A (GA: > 80% normal sperm and >70% motility) and group B (GB: < 80% normal sperm and < 70% motility). Each ejaculate was collected and semen volume, concentration, sperm motility (computer aided semen analysis; CASA), morphology, and vitality (both eosin nigrosin staining) were investigated. The SP was separated and analyzed for aspartate-amino-transferase (AST), γ-glutamyl-transferase (GGT), alkaline phosphatase (ALP) activity, and the concentrations of sodium (Na), potassium (K), chloride (Cl), calcium (Ca), phosphate (PO 4 3-), magnesium (Mg), selenium (Se) and zinc (Zn) were assessed. Repeated measures (2 months interval) were conducted in eight boars of GA from one AI center. The activity of GGT (r = -0.482) and ALP (r = -0.459) was moderately associated ( p < 0.05) with ejaculate volume and strongly associated with concentration (r = 0.580 and r = 0.618, respectively; p = 0.000). Moderate associations ( p < 0.05) were found between ALP (r = 0.439), GGT (r = 0.387), Na (r = -0.428), K (r = 0.354), and Se (r = 0.354) with progressive motility. The SP concentration of Na (r = -0.401), Cl (r = -0.521), and K (r = 0.350) was associated ( p < 0.05) with normal morphology. Only Mg was associated ( p < 0.05) with membrane damage (r = -0.335). The concentration of Na, Cl, and Zn (1681.0 vs. 1701.0 µg/dL) was different between groups ( p < 0.05). Repeated measures showed significant differences in time but only for Na, Mg, and Zn ( p < 0.05). In conclusion, several biochemical components of SP were related to semen quality. The analysis of biochemical parameters could provide extra information about reproductive health of AI boars.

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“…Taking into account Maes et al (2011), at least 70% of morphologically normal spermatozoa per insemination dose are required to achieve high fertilization rates. However, Vyt et al (2008), reported that morphological characteristics have a small predictive value of sperm fertility.…”

Section: P-valuementioning

confidence: 99%

A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility

et al. 2016

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The present study compared the quality characteristics of boar semen diluted with three extenders of different proposed preservation times (short-term, medium-term and long-term). A part of extended semen was used for artificial insemination on the farm (30 sows/extender), while the remaining part was stored for three days (16-18 °C). Stored and used semen was also laboratory assessed at insemination time, on days 1 and 2 after the collection (day 0). The long-term extender was used for a short time, within 2 days from semen collection, with the aim to investigate a possible advantage over the others regarding laboratory or farm fertility indicators at the beginning of the preservation time. Viability, motility, kinetic indicators, morphology and DNA fragmentation were estimated. The results showed reduced viability, higher values for most of the kinetics, and higher immotile spermatozoa from day 1 to day 2 in all extenders; however, the long-term extender was superior compared to the other two on both days. With regard to morphology and chromatin integrity, the percentage of abnormal and fragmented spermatozoa increased on day 2 compared to day 1 for all of the extenders. However, based on the farrowing rate and the number of piglets born alive after the application of conventional artificial insemination within 2 days from semen collection/dilution, it was found that the medium-term diluents were more effective. In conclusion, it seems that the in vivo fertilization process involves more factors than simply the quality of laboratory evaluated sperm indicators, warranting further research. DNA fragmentation, ejaculate, in vitro quality, in vivo fertility, semen analysis, semen storageConventional examination of boar sperm on commercial farms aims to evaluate the fertilizing capacity and to control the conditions of breeding and sperm manipulation after the collection of the ejaculate (Tsakmakidis et al. 2010). Semen quality characteristics are affected by many factors, including the type of semen extender, storage duration and temperature (Fraser and Strzezek 2004).Boar semen diluents are classified by the commercial providers into three categories: short-term (ST), medium-term (MT) and long term (LT) extenders according to their preservation capacity (1-2, 3-4 and 7-10 days after collection, respectively). Long-term extenders allow for long distance sperm transportation. Moreover, the acquired time flexibility enables collection centres to conduct specialized diagnostic tests and to better organize semen procedures (Gadea 2003).In literature, only few studies compare the preservation capacity of different extenders in the laboratory and the relation with actual field conditions. To our knowledge, data on the efficacy of swine artificial insemination (AI) 1-2 days from collection and dilution in long-term extenders are even scarcer. This category of extenders is supposed to maintain sperm quality within acceptable levels (i.e. total sperm number > 35 × 10 9 sperm/ejaculate, gross motility > 70%, abnorma...

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Artificial Insemination in Pigs

Cited by 29 publications

References 58 publications

Artificial insemination in pigs today

Artificial insemination in pigs today

Boar seminal plasma components and their relation with semen quality

A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility

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