Is ozone pre-conditioning effect linked to Nrf2/EpRE activation pathway in vivo? A preliminary result

Re, Lamberto; Martı́nez-Sánchez, Gregorio; Bordicchia, Marica; Malcangi, Giuseppe; Pocognoli, Antonella; Morales-Segura, Miguel Angel; Rothchild, John; Rojas, Armando

doi:10.1016/j.ejphar.2014.08.029

Cited by 89 publications

(94 citation statements)

References 23 publications

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“…Experimental results demonstrated that ozone at therapeutic dosages ex vivo or in vivo can activate Nrf2 [20,32] that involve an indirect modulation (inhibition) of the NF-κB pathway. In addition, Nrf2 suppresses NF-κB activity by eliminating ROS, which may cause NF-κB activation via antioxidative protein induction, such as HO-1 and NQO1.…”

Section: Ozone Therapy As Redox Modulatormentioning

confidence: 99%

“…The paradoxical mechanism by which ozone (a potent oxidant) can induce an antioxidant response, is currently demonstrated not only at a proteomic level, but also at a genomic one. Ozone applied at a therapeutic dose modulates the nuclear factor Nrf2 and NF-κB and induces the homeostasis of the antioxidant environment [18,[29][30][31][32]. Oxidative stress and innate immunity have a key role in lung injury pathways that control the severity of acute lung cytotoxicity during viral infections like SARS [33].…”

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confidence: 99%

“…The induction of antiviral cytokines such as IFN and the modulation of pro-inflammatory cytokines as IL-6, have been demonstrated by ozonating blood such as major autohemotherapy (MAH). Although ozone is a weak inducer, reinfused lymphocytes and monocytes during mayor autohemotherapy (MAH), can migrate through the lymphoid system, and activate other cells that, in time, will lead to the stimulation of the immune system [32,34]. This may represent an important process because it is known that an acute viral disease becomes more severe because the virus is particularly virulent, the heterogeneous viral population evolves rapidly and escapes immune control, or because the immune system becomes tolerant to viral antigens and becomes unable to counteract the infection.…”

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confidence: 99%

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Potential Cytoprotective Activity of Ozone Therapy in SARS-CoV-2/COVID-19

2020

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1) Background: The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease in China at the end of 2019 has caused a large global outbreak. Systemic ozone therapy (OT) could be potentially useful in the clinical management of several complications secondary to SARS-CoV-2. The rationale and mechanism of action has already been proven clinically in other viral infections and has been shown in research studies to be highly effective at decreasing organ damage mediated by inflammation and oxidative stress. This review summarizes the OT studies that illustrate the possible cytoprotective mechanism of action of ozone and its physiological by-products in target organs affected by SARS-CoV-2. (2) Methods: This review encompasses a total of 74 peer-reviewed original articles. It is mainly focused on ozone as a modulator of the NF-κB/Nrf2 pathways and IL-6/IL-1β expression. (3) Results: In experimental models and the few existent clinical studies, homeostasis of the free radical and antioxidant balance by OT was associated with a modulation of NF-κB/Nrf2 balance and IL-6 and IL-1β expression. These molecular mechanisms support the cytoprotective effects of OT against tissue damage present in many inflammatory diseases, including viral infections. (4) Conclusions: The potential cytoprotective role of OT in the management of organ damage induced by COVID-19 merits further research. Controlled clinical trials are needed.

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Section: Ozone Therapy As Redox Modulatormentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Potential Cytoprotective Activity of Ozone Therapy in SARS-CoV-2/COVID-19

2020

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“…Nuclear factor erythroid-2-related factor 2 (Nrf2) is a nuclear transcription factor with neuroprotective effects against central nervous system disease (3). The Nrf2 pathway is anti-apoptotic and has multiple functions, including resistance to oxidative stress, regulation of inflammatory injury and relief from calcium overloading (4).…”

Section: Introductionmentioning

confidence: 99%

Curcumin attenuates hypoxic-ischemic brain injury in neonatal rats through induction of nuclear factor erythroid-2-related factor 2 and heme oxygenase-1

Cui

Song

Shi

2017

Experimental and Therapeutic Medicine

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Curcumin has previously demonstrated anti-inflammatory, anti-infective and immuno-suppressive effects. In the present study, whether the attenuating effects of curcumin against hypoxic-ischemic brain injury in neonatal rats are mediated via nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was investigated. A model of hypoxic-ischemic brain injury was created using 1-week-old Sprague Dawley rats (weight, 52±1 g). The model rats were treated with 150 mg/kg curcumin by gavage for 3 days. Malondialdehyde levels, and superoxide dismutase and caspase-3 activities were assayed using commercial kits and western blot analysis was used to measure inducible nitric oxide synthase (iNOS), Nrf2 and HO-1 expression levels. Treatment with curcumin effectively reduced the brain injury score, increased myelin basic protein (MBP) expression and increased the quantity of neuronal cells in neonatal rats with hypoxic-ischemic brain injury. Furthermore, treatment with curcumin significantly attenuated the changes in SOD activity and MDA levels and suppressed the iNOS protein expression induced in neonatal rats by hypoxic-ischemic brain injury. Treatment with curcumin significantly increased Nrf2 and HO-1 expression in the neonatal rats with hypoxic-ischemic brain injury. The present study indicated that curcumin attenuates hypoxic-ischemic brain injury in neonatal rats through the induction of Nrf2 and HO-1.

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“…Human studies [7] have been performed to evaluate some interesting metabolic pathways activated by a correct administration of ozone doses using the most appropriate technique. We imagine that very many major autohemotherapies must have been administered so far all around the world by the thousands of physicians working in this field.…”

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confidence: 99%