Is molecular size a discriminating factor in hyaluronan interaction with human cells?

D’Agostino, Antonella; Stellavato, Antonietta; Corsuto, Luisana; Paola, Diana; Filosa, Rosanna; Gatta, Annalisa La; Rosa, Mario De; Schiraldi, Chiara

doi:10.1016/j.carbpol.2016.07.125

Cited by 74 publications

(71 citation statements)

References 42 publications

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“…Additional time-lapse experiments using the lowest HA molecular weight preparation were performed and a significant increase in the wound repair capacity was still observed. This is in line with previous findings demonstrating that, except for very low molecular weight fragments (< 15kDa), HA fastens the wound healing of human keratinocytes regardless of the molecular weight and that only a slight reduction in the rate of wound closure occurs with the decrease in HA chains length [20]. We conclude that the interlot variation found in Aminogam® influences, to a certain extent, the physical properties related to HA molecular weight (i.e.…”

Section: Discussionsupporting

confidence: 93%

“…We have shown recently that high-and low-molecular weight chains of hyaluronan and their hybrid H-HA/L-HA complexes accelerate wound healing of HaCaT cells seeded on collagen-coated plates [19,20]. In this present study, we used uncoated plates to show that Aminogam® promotes keratinocyte migration regardless of integrin-collagen engagement.…”

Section: Discussionmentioning

confidence: 75%

“…The 'wound closure' phenomenon was monitored for 48h using TLVM station to observe on line the phenomena of HaCaT monolayer migrating to repair the wound, in the control and contemporary in presence of Aminogam®. The instrument is equipped with a motorized CO 2 stage incubator that allows, once selected an image, to return on the same field of view in times defined by the operator [20]. A quantitative analysis of the rate of wound closure was obtained.…”

Section: Dynamic Viscosity Measurementsmentioning

confidence: 99%

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A biophysically-defined hyaluronic acid-based compound accelerates migration and stimulates the production of keratinocyte-derived neuromodulators

Gatta

D’Agostino

Schiraldi

et al. 2018

Cell Adhesion & Migration

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Hyaluronic acid (HA) preparations are widely used in clinical practice and recent data suggest that commercially available HA-based compounds promote ulcer re-epithelialization and induce pain relief. However, the pathophysiological basis of these effects remains poorly understood. In the present study, we investigated the biophysical, biomolecular and functional properties of a HA preparation combined with a pool of collagen precursor synthetic aminoacids, namely l-proline, l-leucine, l-lysine and glycine (Aminogam®). Hydrodynamic characterization of Aminogam® by size exclusion chromatography-triple detector array (SEC-TDA) revealed an average molecular weight in the range of 700-1700 kDa. Rheological measurements of the 1700kDa M lot showed a pseoudoplastic behaviour with a zero-shear viscosity (η) equal to 90 ± 9 Pa∙s at 25°C and 55 ± 6 Pa∙s at 37°C. Automated time-lapse videomicroscopy studies in a fibroblast-free system demonstrated that 1% (v/v) Aminogam® significantly reduced the healing time of wounded keratinocyte monolayers. In AKGOS assays, Aminogam® stimulated cellular locomotion (chemokinesis) and directional migration (chemotaxis) of keratinocytes. Analysis of microarray data suggested that keratinocytes had a functional neuroendocrine machinery, and this was confirmed by testing the secretion of six neuroactive molecules by ELISA, namely α-MSH, β-endorphins, melatonin, substance P, cortisol, and neurotensin. Interestingly, Aminogam® regulated the production of several neuropeptides, including β-endorphins. In conclusion, our data shed light on the epithelial-dependent mechanisms that underlie the efficacy of Aminogam®, particularly in reference to wound healing and nociception.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 75%

Section: Dynamic Viscosity Measurementsmentioning

confidence: 99%

See 1 more Smart Citation

A biophysically-defined hyaluronic acid-based compound accelerates migration and stimulates the production of keratinocyte-derived neuromodulators

Gatta

D’Agostino

Schiraldi

et al. 2018

Cell Adhesion & Migration

Self Cite

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“…HA with a molecular weight higher than 6 kDa would inhibit angiogenesis. [21][22][23] In previous studies of HA-coated liposomes, H. S. S. Qhattal et al provided evidence that HA-liposomes were taken up into cells via CD44 receptor-controlled endocytosis, and the cellular uptake was increased when compared with PEG-liposomes. 24 However, HA-liposomes showed a different cellular targeting efficiency, which depended strongly upon the HA MW and graing density, indicating its varied binding affinity to the CD44 receptor.…”

Section: Introductionmentioning

confidence: 99%

“…[24][25][26] In this study, we selected two MWs of HA (380 and 102 kDa), respectively named as h-HA and l-HA, to investigate the targeting ability of different MWs of HA-coated gold nanobipyramids (GBPs) for TNBC. 21 GBPs have raised much attention because of their superior optical properties, larger extinction cross section, and more signicant local electric-eld enhancement than gold nanorods. [27][28][29] Different from the rounded ends of gold nanorods, GBPs have two sharper apexes rising onto the cross point.…”

Section: Introductionmentioning

confidence: 99%

High and low molecular weight hyaluronic acid-coated gold nanobipyramids for photothermal therapy

et al. 2018

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Tailoring Hyaluronic Acid Hydrogels for Biomedical Applications

Luo,

Wang,

et al. 2023

Adv Funct Materials

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Hyaluronic acid (HA) is an attractive anionic polysaccharide polymer with inherent pharmacological properties and versatile chemical groups for modification. Due to their water retention ability, biocompatibility, biodegradation, cluster of differentiation‐44 targeting, and highly designable capacity, HA hydrogels have been an emerging biomaterial, showing tailoring performance in terms of chemical modifications and hydrogel forms. Various preparation technologies have been developed for the fabrication of the tailoring HA hydrogels with unique structures and functions. They have been utilized in diverse biomedical applications like drug delivery and tissue engineering scaffolds. Herein, this review comprehensively summarizes the HA derivatives with different molecule weights and functional modifications. Then the various fabrication methods to obtain tailoring hydrogels in the forms of nanogel, nanofiber, microparticle, microneedle patch, injectable hydrogel, and scaffold are reviewed as well. The emphasis is focused on the shining biomedical applications of these tailoring HA hydrogels in anti‐bacteria, anti‐inflammation, wound healing, cancer treatment, regenerative medicine, psoriasis treatment, diagnosis, etc. The potentials and prospects are subsequently given to inspire further investigation, aiming at accelerating product translation from research to clinic.

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Is molecular size a discriminating factor in hyaluronan interaction with human cells?

Cited by 74 publications

References 42 publications

A biophysically-defined hyaluronic acid-based compound accelerates migration and stimulates the production of keratinocyte-derived neuromodulators

A biophysically-defined hyaluronic acid-based compound accelerates migration and stimulates the production of keratinocyte-derived neuromodulators

High and low molecular weight hyaluronic acid-coated gold nanobipyramids for photothermal therapy

Tailoring Hyaluronic Acid Hydrogels for Biomedical Applications

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