Is microRNA 1910-3p (miR-1910-3p) a really distinctive marker for psoriasis?

Karabacak, Melek; Erturan, İ̇jlal; Öztürk, Kuyaş Hekimler; Ayvaz, Havva Hilal; Korkmaz, Selma; Yıldırım, Mehmet; Orhan, Hikmet

doi:10.3906/sag-2009-156

Cited by 2 publications

(1 citation statement)

References 25 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We reported that serum miR-1910-3p levels were significantly lower and miR-4649-3p levels were significantly higher in the patient group. Furthermore, the decreased expression levels of miR-1910-3p could differ psoriatic patients from healthy controls [31]. In Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi the present study, Although there was no significant difference between the patient and the healthy control group, it was shown in the logistic regression analysis that the decrease in miR-1910-3p may be significant in the development of the disease.…”

Section: Discussioncontrasting

confidence: 45%

The Possible Role of Mir-1910-3p, Mir-4649-3p, Mir-4296, and Mir-210 in the Pathogenesis of Atopic Dermatitis: May Mir-4296 Play Crucial Roles in the Development of Atopic Dermatitis?

Ayvaz

Öztürk

Atay

et al. 2022

SDÜ Tıp Fakültesi Dergisi

Self Cite

View full text Add to dashboard Cite

Objective: Atopic dermatitis (AD) is a chronic inflammatory skin disease with unexplained points in its pathogenesis. Altered expressions of microRNAs (miRNA, miR) in plasma can serve as markers that distinguish diseased individuals from healthy controls AD. In the present study, plasma expression levels of miR-1910-3p, miR-4649-3p, miR-4296 and miR-210 were investigated in AD. Material and Methods: Forty patients with AD and forty healthy control subjects were included in the study. Quantitative real-time PCR was used to measure miRNAs. Results: The mean plasma miR-4296 level was significantly higher in the patient group (p < 0.001). There was a statistically significant negative correlation between SCORAD scores and miR-210 levels (r:-0.340, p=0.032). miR-210 levels decreased with increasing disease severity. In logistic regression analyses, an increase in plasma miR-4296 levels and a decrease in plasma miR-1910-3p levels were found to be statistically significant. Conclusion: MiRNAs are crucial in the pathogenesis of AD. Increased miR-4296 and decreased miR-1910-3p expression levels seem to be significantly better at discriminating AD patients from healthy controls.

show abstract

Section: Discussioncontrasting

confidence: 45%

The Possible Role of Mir-1910-3p, Mir-4649-3p, Mir-4296, and Mir-210 in the Pathogenesis of Atopic Dermatitis: May Mir-4296 Play Crucial Roles in the Development of Atopic Dermatitis?

Ayvaz

Öztürk

Atay

et al. 2022

SDÜ Tıp Fakültesi Dergisi

Self Cite

View full text Add to dashboard Cite

show abstract

Vitamin D receptor-deficient keratinocytes-derived exosomal miR-4505 promotes the macrophage polarization towards the M1 phenotype

Sun

Chen

Li³

et al. 2023

PeerJ

View full text Add to dashboard Cite

Background The vitamin D receptor (VDR) has a low level of expression in the keratinocytes of patients with psoriasis and plays a role in the development of the disease. Furthermore, the crosstalk between macrophages and psoriatic keratinocytes-derived exosomes is critical for psoriasis progression. However, the effects of VDR-deficient keratinocytes-derived exosomes (Exos-shVDR) on macrophages and their underlying mechanisms remain largely unknown. Methods VDR-deficient keratinocytes were constructed by infecting HaCaT cells with a VDR-targeting lentivirus, mimicking the VDR-deficient state observed in psoriatic keratinocytes. Exosomes were characterized using transmission electron microscopy, nanoparticle tracking analysis, and Western blot. The effect of Exos-shVDR on macrophage proliferation, apoptosis, and M1/M2 polarization was assessed using cell counting kit-8 assay (CCK-8), flow cytometer, real-time quantitative polymerasechain reaction (RT-qPCR), and enzyme-linked immunosorbent assay (ELISA). The mechanism underlying the effect of Exos-shVDR on macrophage function was elucidated through data mining, bioinformatics, RT-qPCR, and rescue experiments. Results Our results revealed that both Exos-shVDR and Exos-shNC exhibited typical exosome characteristics, including a hemispheroid shape with a concave side and particle size ranging from 50 to 100 nm. The levels of expression of VDR were significantly lower in Exos-shVDR than in Exos-shNC. Functional experiments demonstrated that Exos-shVDR significantly promoted macrophage proliferation and polarization towards the M1 phenotype while inhibiting macrophage apoptosis. Moreover, miR-4505 was highly expressed in the skin tissue of patients with psoriasis. Its overexpression significantly increased macrophage proliferation and polarization towards M1 and inhibited apoptosis. Furthermore, the effects of Exos-shVDR on macrophage function occur through miR-4505. Conclusions Exos-shVDR exacerbates macrophage proliferation, promotes polarization towards the M1 phenotype, and inhibits macrophage apoptosis by increasing the levels of miR-4505. These results indicate that modulation of macrophage function is a potential strategy for developing new drugs for the treatment of psoriasis.

show abstract

Is microRNA 1910-3p (miR-1910-3p) a really distinctive marker for psoriasis?

Cited by 2 publications

References 25 publications

The Possible Role of Mir-1910-3p, Mir-4649-3p, Mir-4296, and Mir-210 in the Pathogenesis of Atopic Dermatitis: May Mir-4296 Play Crucial Roles in the Development of Atopic Dermatitis?

The Possible Role of Mir-1910-3p, Mir-4649-3p, Mir-4296, and Mir-210 in the Pathogenesis of Atopic Dermatitis: May Mir-4296 Play Crucial Roles in the Development of Atopic Dermatitis?

Vitamin D receptor-deficient keratinocytes-derived exosomal miR-4505 promotes the macrophage polarization towards the M1 phenotype

Contact Info

Product

Resources

About