Is Leishmania (Viannia) braziliensis preferentially restricted to the cutaneous lesions of naturally infected dogs?

Madeira, Maria de Fátima; Schubach, Armando de Oliveira; Schubach, Tânia Maria Pacheco; Serra, Carlos Henrique Aguiar; Pereira, Sandro Antônio; Figueiredo, Fabiano Borges; Confort, Eliame Mouta; Quintella, Leonardo Pereira; Marzochi, Mauro Célio de Almeida

doi:10.1007/s00436-005-1374-y

Cited by 20 publications

(18 citation statements)

References 21 publications

(20 reference statements)

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“…In Paracatu, Minas Gerais, 5% out of 267 dogs with positive serology for VL were asymptomatic (Dias et al 2011). The presence of L. infantum amastigotes in the skin of asymptomatic seropositive dogs has been reported previously (Lainson & Rangel 2005, Madeira et al 2005, Dias et al 2011. In this study, parasitological examination showed the presence of amastigotes in bone marrow (100%), spleen and liver (54.5%), lymph nodes (45.4%) and skin (90.9%) in all asymptomatic dogs.…”

Section: Discussionmentioning

confidence: 70%

Outbreak of autochthonous canine visceral leishmaniasis in Santa Catarina, Brazil

Steindel

Menin

Evangelista³

et al. 2013

Pesq. Vet. Bras.

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The present study reports the first outbreak of autochthonous canine visceral leishmaniasis in Florianópolis, Santa Catarina, southern Brazil. Following the report of two cases of CVL, the Control Center of Zoonotic Diseases conducted a serological survey by ELISA and IFAT assays in seven districts of the Santa Catarina Island. Eleven seropositive dogs of autochthonous transmission were used in the present study. Infection by Leishmania sp. was confirmed by parasitological examination of bone marrow, liver, spleen and lymph nodes, culture in Schneider's medium and PCR. Leishmania sp. isolates were characterized by PCR-RFLP and hybridization with specific probes, allowing for the identification of Leishmania infantum. Autochthonous transmission of this disease in an area with high tourist traffic presents a major public health concern and signifies the emergence of an important zoonosis in southern Brazil. Therefore, the implementation of surveillance and control measures is imperative to prevent the spread of the disease among the canine population as well as transmission to the human population. infecção por Leishmania sp. foi realizada pelo exame parasitológico da medula óssea, fígado, baço e linfonodos, cultura em meio Schneider e PCR. Os isolados de Leishmania sp. foram caracterizados por PCR-RFLP e hibridação com sondas específicas, permitindo a identificação de Leishmania infantum. A transmissão autóctone da LCV em uma área com grande fluxo turístico como Florianópolis representa um preocupante risco à saúde pública e o surgimento de uma importante zoonose no sul do Brasil. Neste contexto, a implementação de medidas de vigilância e controle da doença são fundamentais para evitar a propagação da doença entre a população canina, bem como a transmissão para a população humana.TERMOS DE INDEXAÇÃO: Leishmaniose visceral canina, Leishmania infantum, zoonose, doenças negligenciadas, saúde pública.

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Section: Discussionmentioning

confidence: 70%

Outbreak of autochthonous canine visceral leishmaniasis in Santa Catarina, Brazil

Steindel

Menin

Evangelista³

et al. 2013

Pesq. Vet. Bras.

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“…Some authors have only isolated parasites from skin lesions (MADEIRA et al, 2005(MADEIRA et al, , 2006CASTRO et al, 2007). Moreover, Castro et al (2007) suggested that dogs are not good reservoirs for L. (V.) braziliensis and Massunari et al (2009) suggested that dogs have a secondary role in the transmission cycle of this parasite.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological profile of cutaneous leishmaniasis in an endemic region in the State of Rio de Janeiro, Brazil

Bustamante¹,

Pereira²,

Schubach³

et al. 2009

RBPV

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The objective of the present study was to investigate the dynamics and profile of American Cutaneous Leishmaniasis (ACL) in an old colonization area in the State of Rio de Janeiro. Health departments of municipalities in the Central-Southern region that had notified cases to the Ministry of Health's Notifiable Diseases Information System between 1997 and 2002 were contacted to obtain data. Out of the 119 cases recorded, 51 patients were visited for an interview and inspection of the environment. The cases of ACL exhibited a profile similar to that observed in other Brazilian cities, affecting individuals of both genders, all age groups and different occupational categories, especially students. Risk activities were reported by 56.9% of the interviewees and 84.3% had never left their homeland. Water courses, chicken coops, bamboo plantations and different fruit trees including banana plants and mango trees were frequently observed in the surrounding environment. Most of the interviewees had not noted any environmental changes prior to becoming sick. Domestic animals were present in all homes, but only dogs presented lesions suggestive of the disease. These were on different occasions that rarely preceded or coincided with the human cases. The possible existence of distinct transmission cycles, i.e. one for canines and another for humans, was discussed.Keywords: American Cutaneous Leishmaniasis, Leishmania (Viannia) braziliensis, zoonotic diseases, epidemiology. ResumoEste estudo teve como objetivo conhecer a dinâmica e o perfil da Leishmaniose Tegumentar Americana (LTA) em uma área de colonização antiga do Estado do Rio de Janeiro. As secretarias de saúde dos municípios da Região Centro-Sul que notificaram casos ao Sistema de Informação de Agravos de Notificação do Ministério da Saúde entre os anos de 1997 e 2002 foram contactadas para obtenção dos dados. Do total de 119 casos registrados, 51 pacientes foram visitados para realização de entrevista e observação do ambiente. A LTA exibiu perfil semelhante ao de outras cidades brasileiras, acometendo indivíduos de ambos os sexos, de todas as faixas etárias e diversas categorias ocupacionais, principalmente estudantes. A realização de atividades de risco foi relatada por 56,9% dos entrevistados e 84,3% não se deslocaram do município de residência. No ambiente circundante havia, frequentemente, cursos d'água, galinheiros, bambuzais e diversas frutíferas, entre elas: bananeiras e mangueiras. A maioria dos entrevistados não observou mudanças ambientais anteriormente ao adoecimento. Animais domésticos estiveram presentes em todos os domicílios, mas apenas cães evidenciaram lesões sugestivas da doença em ocasiões diversas, raramente antecedendo ou coincidindo com casos humanos. A possibilidade de existirem ciclos de transmissão distintos, um canino e outro, humano, foi discutida.

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“…In wild and synantropic animals (considered potential reservoirs for L. braziliensis), such a distinction regarding tissue tropism does not exist (ROQUE et al, 2010;ROQUE & JANSEN, 2014), and the parasite may be detected in many organs. In dogs, this species has only been detected in cutaneous lesions (MADEIRA et al, 2005;DANTAS-TORRES, 2007). The visceralization detected herein by L. braziliensis may be a consequence of the impaired immune system of the dogs.…”

Section: Discussionmentioning

confidence: 69%

“…Cross-reactivity using Leishmania infantum antigen with sera from Leishmania braziliensis-infected dogs is commonly observed (BRASIL, 2006), but dog culling, in this case, is not recommended. Dogs infected with L. braziliensis commonly present localized skin lesions without systemic dissemination (MADEIRA et al, 2005). The urban reservoir of L. braziliensis is still not known in Brazil.…”

Section: Introductionmentioning

confidence: 99%

Hepatozoon canis and Leishmania spp. coinfection in dogs diagnosed with visceral leishmaniasis

Morgado

Cavalcanti

Miranda

et al. 2016

Rev. Bras. Parasitol. Vet.

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This study describes the occurrence of dogs naturally co-infected with Hepatozoon canis and two Leishmania species: L. infantum or L. braziliensis. Four dogs serologically diagnosed with Visceral Leishmaniasis were euthanized. Liver and spleen samples were collected for histopathological analysis and DNA isolation. H. canis meronts were observed in tissues from all four dogs. H. canis infection was confirmed by PCR followed by sequencing of a fragment of 18S rRNA gene. Leishmania detection and typing was confirmed by ITS1' PCR-RFLP and parasite burden was calculated using ssrRNA quantitative qPCR. A DPP -Dual Path platform test was performed. One out (Dog #2) of four animals was asymptomatic. Dogs #1 and #4 were infected by L. infantum and were DPP test positive. Dogs #2 and #3 were infected by L. braziliensis and were DPP test negative. Furthermore, visceral dissemination was observed in Dogs #2 and #3, since L. braziliensis was detected in liver and spleen samples. The visceral dissemination of L. braziliensis associated with systemic signs suggested that this co-infection could influence the parasite burden and disease progression.Keywords: Hepatozoonosis, canine visceral leishmaniasis, Leishmania infantum, Hepatozoon canis, co-infection, Leishmania braziliensis. ResumoO presente estudo descreve a ocorrência de coinfecção com Hepatozoon canis e duas espécies de Leishmania (L. infantum ou L. braziliensis) em cães. Quatro cães sorologicamente diagnosticados com leishmaniose visceral foram eutanasiados. Amostras do baço e fígado foram submetidas à histopatologia e extração de DNA. Merontes de H. canis foram observados nos quatro cães. A infecção por H. canis foi confirmada por PCR e sequenciamento de um fragmento do gene 18S rRNA. A infecção por Leishmania e tipagem foram realizadas por PCR-RFLP do região intergênica ITS1. A carga parasitária foi calculada pela qPCR quantitativa baseada no gene ssrRNA. O teste DPP -Dual Path platform foi realizado. Apenas o Cão #2 era assintomático. Os cães #1 e #4 estavam infectados com L. infantum e foram positivos no DPP. Os cães #2 e #3 estavam infectados com L. braziliensis e foram negativos no DPP. Além disso, visceralização foi observada nos cães #2 e #3, nos quais L. braziliensis foi detectada em amostras de baço e fígado. A visceralização da L. braziliensis associada a sinais clínicos sistêmicos sugerem que esta coinfecção pode ter influenciado na carga parasitária e progressão da doença.Palavras-chave: Hepatozoonose, leishmaniose visceral canina, Leishmania infantum, Hepatozoon canis, coinfecção, Leishmania braziliensis.

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Is Leishmania (Viannia) braziliensis preferentially restricted to the cutaneous lesions of naturally infected dogs?

Cited by 20 publications

References 21 publications

Outbreak of autochthonous canine visceral leishmaniasis in Santa Catarina, Brazil

Outbreak of autochthonous canine visceral leishmaniasis in Santa Catarina, Brazil

Epidemiological profile of cutaneous leishmaniasis in an endemic region in the State of Rio de Janeiro, Brazil

Hepatozoon canis and Leishmania spp. coinfection in dogs diagnosed with visceral leishmaniasis

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