Is it feasible to control pathogen infection by competitive binding of probiotics to the host?

Fukuda, Kenji

doi:10.1080/21505594.2017.1382798

Cited by 7 publications

(9 citation statements)

References 50 publications

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“…Interaction between molecules distributed in the gut epithelia and the surface of bacterial cells mediates the adhesion and colonization of bacteria. Commensal or probiotic bacteria produce adhesive surface molecules (e.g., enolases, glyceraldehyde-3-phosphate, and pyruvate dehydrogenase) and adhere to the extracellular matrix of the host (33,34). These adhesive surface molecules assist commensal bacteria and probiotics in contesting and preventing pathogenic bacterial attachment and colonization (35,36).…”

Section: Competitive Pathogen Exclusionmentioning

confidence: 99%

Modulatory Effects of Probiotics During Pathogenic Infections With Emphasis on Immune Regulation

et al. 2021

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In order to inhibit pathogenic complications and to enhance animal and poultry growth, antibiotics have been extensively used for many years. Antibiotics applications not only affect target pathogens but also intestinal beneficially microbes, inducing long-lasting changes in intestinal microbiota associated with diseases. The application of antibiotics also has many other side effects like, intestinal barrier dysfunction, antibiotics residues in foodstuffs, nephropathy, allergy, bone marrow toxicity, mutagenicity, reproductive disorders, hepatotoxicity carcinogenicity, and antibiotic-resistant bacteria, which greatly compromise the efficacy of antibiotics. Thus, the development of new antibiotics is necessary, while the search for antibiotic alternatives continues. Probiotics are considered the ideal antibiotic substitute; in recent years, probiotic research concerning their application during pathogenic infections in humans, aquaculture, poultry, and livestock industry, with emphasis on modulating the immune system of the host, has been attracting considerable interest. Hence, the adverse effects of antibiotics and remedial effects of probiotics during infectious diseases have become central points of focus among researchers. Probiotics are live microorganisms, and when given in adequate quantities, confer good health effects to the host through different mechanisms. Among them, the regulation of host immune response during pathogenic infections is one of the most important mechanisms. A number of studies have investigated different aspects of probiotics. In this review, we mainly summarize recent discoveries and discuss two important aspects: (1) the application of probiotics during pathogenic infections; and (2) their modulatory effects on the immune response of the host during infectious and non-infectious diseases.

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Section: Competitive Pathogen Exclusionmentioning

confidence: 99%

Modulatory Effects of Probiotics During Pathogenic Infections With Emphasis on Immune Regulation

et al. 2021

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“…It is covered by mucus that accommodates the majority of the microbiota and is the place for the attachment of bacteria, being either probiotic, commensal or pathogenic, and intimate encounters with the host. The mucus ( Figure 1 ) is a gel layer serving for hydration and lubrication as well as a barrier against pathogens and harmful substances [ 14 , 15 , 16 ]. It consists mostly of water, glycoproteins, salts, and lipids.…”

Section: Gastrointestinal Tract Mucins and Lactobacillimentioning

confidence: 99%

“…Lactobacilli can exert health benefits in several ways, for example by inhibiting growth of pathogenic bacteria as they produce lactic, propionic, and acetic acids lowering the pH that suppresses proliferation of pathogens in the GIT [ 20 , 30 , 31 ]. Additionally, they can competitively prevent attachment of pathogens to the epithelium [ 16 , 32 , 33 , 34 , 35 ]. Lactobacilli have also been connected with improvements of gut diseases or disease-associated symptoms.…”

Section: Gastrointestinal Tract Mucins and Lactobacillimentioning

confidence: 99%

“…Besides pili proteins, which are situated on the bacterial surface and have a canonical function in cell-cell and cell-extracellular matrix protein interactions, lactic acid bacteria contain a large number of proteins referred to as moonlighting proteins, which have been demonstrated to act in adhesion [ 16 , 66 , 68 ] ( Figure 5 ). Moonlighting proteins are multifunctional proteins that participate in unrelated biological processes and which are not the result of gene fusion.…”

Section: Adhesion Factors Of Lactobacillimentioning

confidence: 99%

“…They are anchorless and the mechanism of the export to the surface has not been identified, but it was proposed to occur eitherby non-canonical secretion, as these proteins lack a signal motif, or by binding of cytosolic proteins from lysed cells onto the cell wall of intact bacteria, although this latter mechanism recently received reduced support [ 5 , 61 , 68 , 69 , 70 ]. Moonlighting proteins comprise a subset of multifunctional proteins belonging to different categories, metabolic enzymes, molecular chaperones, translational elongation factors, ribosomal and other proteins [ 16 , 29 , 50 , 66 , 68 , 69 , 71 ]. Following simulated GIT passage by exposure to bile and acidic pH, L. paracasei CIDCA 83123 showed clearly increased capacity to adhere to mucin and Caco-2 cells.…”

Section: Adhesion Factors Of Lactobacillimentioning

confidence: 99%

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Dietary Nutrients, Proteomes, and Adhesion of Probiotic Lactobacilli to Mucin and Host Epithelial Cells

Çelebioğlu

Svensson

2018

Microorganisms

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The key role of diet and environment in human health receives increasing attention. Thus functional foods, probiotics, prebiotics, and synbiotics with beneficial effects on health and ability to prevent diseases are in focus. The efficacy of probiotic bacteria has been connected with their adherence to the host epithelium and residence in the gut. Several in vitro techniques are available for analyzing bacterial interactions with mucin and intestinal cells, simulating adhesion to the host in vivo. Proteomics has monitored and identified proteins of probiotic bacteria showing differential abundance elicited in vitro by exposure to food components, including potential prebiotics (e.g., certain carbohydrates, and plant polyphenols). While adhesion of probiotic bacteria influenced by various environmental factors relevant to the gastrointestinal tract has been measured previously, this was rarely correlated with changes in the bacterial proteome induced by dietary nutrients. The present mini-review deals with effects of selected emerging prebiotics, food components and ingredients on the adhesion of probiotic lactobacilli to mucin and gut epithelial cells and concomitant abundancy changes of specific bacterial proteins. Applying this in vitro synbiotics-like approach enabled identification of moonlighting and other surface-located proteins of Lactobacillus acidophilus NCFM that are possibly associated with the adhesive mechanism.

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The probiotic potential of Lactobacillus plantarum strain RW1 isolated from canine faeces

Raheem

Wang

Zhang

et al. 2022

Journal of Applied Microbiology

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To evaluation the probiotic potential of Lactobacillus plantarum strain RW1 isolated from healthy dogs for its further utilization as a dietary supplement for dogs. Methods and Results:This study aimed to evaluate the probiotic potential of L. plantarum strain RW1 isolated from canine faeces. After confirming by conventional and then by 16S rRNA sequencing, the identified strain RW1 was in vitro screened for its survivability in simulated gastrointestinal conditions, low pH, bile salts and adhesion to gut epithelial tissues, growth inhibitory effects on common pathogens and anti-inflammatory potential by measuring the mRNA expression level of IL-6, IL-8, IL-1β in Salmonella-infected MODE-K cells. Furthermore, the effects on epithelial barrier function and host defensin peptide (beta-defensin 3) was studied by measuring the mRNA expression level of tight junction protein (occludin) and betadefensin 3 in MODE-K cells. The strain RW1 showed a considerable potential to survive in simulated gastrointestinal environmental conditions, low pH and high bile salt concentrations along with good adhesion to MODE-K cell line. Pathogenic bacterial growth and their adhesion to MODE-K cell line were significantly inhibited by the strain RW1. Real-time PCR analyses demonstrated that the strain RW1 inhibited Salmonella-induced pro-inflammatory cytokines (IL-6, IL-8 and IL-1β) production and reinforced the expression of tight junction protein (occludin). The strain RW1 did not induce mRNA expression of beta-defensin 3. Conclusion:Based on in vitro results, the strain RW1 has the potential to be used as a probiotic supplement in dogs. However, further study involving in vivo health effects is needed.Significance and Impact of the Study: Antibiotics have many side effects and nowadays the probiotics are considered as a potential alternative to antibiotics. This study evaluates the probiotic potential of dog isolated L. plantarum strain RW1 to use it as a dietary supplement in dogs feeding to control infectious diseases.

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Is it feasible to control pathogen infection by competitive binding of probiotics to the host?

Cited by 7 publications

References 50 publications

Modulatory Effects of Probiotics During Pathogenic Infections With Emphasis on Immune Regulation

Modulatory Effects of Probiotics During Pathogenic Infections With Emphasis on Immune Regulation

Dietary Nutrients, Proteomes, and Adhesion of Probiotic Lactobacilli to Mucin and Host Epithelial Cells

The probiotic potential of Lactobacillus plantarum strain RW1 isolated from canine faeces

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