The probiotic potential of Lactobacillus plantarum strain RW1 isolated from canine faeces

Abstract

To evaluation the probiotic potential of Lactobacillus plantarum strain RW1 isolated from healthy dogs for its further utilization as a dietary supplement for dogs. Methods and Results:This study aimed to evaluate the probiotic potential of L. plantarum strain RW1 isolated from canine faeces. After confirming by conventional and then by 16S rRNA sequencing, the identified strain RW1 was in vitro screened for its survivability in simulated gastrointestinal conditions, low pH, bile salts and adhesion to gut epithelial tissues, growth inhibitory effects on common pathogens and anti-inflammatory potential by measuring the mRNA expression level of IL-6, IL-8, IL-1β in Salmonella-infected MODE-K cells. Furthermore, the effects on epithelial barrier function and host defensin peptide (beta-defensin 3) was studied by measuring the mRNA expression level of tight junction protein (occludin) and betadefensin 3 in MODE-K cells. The strain RW1 showed a considerable potential to survive in simulated gastrointestinal environmental conditions, low pH and high bile salt concentrations along with good adhesion to MODE-K cell line. Pathogenic bacterial growth and their adhesion to MODE-K cell line were significantly inhibited by the strain RW1. Real-time PCR analyses demonstrated that the strain RW1 inhibited Salmonella-induced pro-inflammatory cytokines (IL-6, IL-8 and IL-1β) production and reinforced the expression of tight junction protein (occludin). The strain RW1 did not induce mRNA expression of beta-defensin 3. Conclusion:Based on in vitro results, the strain RW1 has the potential to be used as a probiotic supplement in dogs. However, further study involving in vivo health effects is needed.Significance and Impact of the Study: Antibiotics have many side effects and nowadays the probiotics are considered as a potential alternative to antibiotics. This study evaluates the probiotic potential of dog isolated L. plantarum strain RW1 to use it as a dietary supplement in dogs feeding to control infectious diseases.

Antioxidant Mechanism of Lactiplantibacillus plantarum KM1 Under H2O2 Stress by Proteomics Analysis

“…However, the number of viable cells of L. plantarum L3C1E8 decreased from 8.84 ± 0.23 to 2.25 ± 0.14 log CFU/ml at pH 2.5 for 2 h, and its survival rate was only 25.45% (Ribeiro et al, 2018 ), which was evidently lower than that of L. plantarum KM1. The survival rate of L. plantarum KM1 cultured at 0.2% bile salt concentration for 2 h was 80.26%, which was significantly higher than L. plantarum RW1 at the same concentration for 3 h (75%) (Raheem et al, 2021 ). Antibiotics including kanamycin sulfate, streptomycin sulfate, tetracycline HCl, and ampicillin trihydrate inhibited protein synthesis (Oh and Jung, 2015 ).…”

Influence of dietary supplementation with new Lactobacillus strains on hematology, serum biochemistry, nutritional status, digestibility, enzyme activities, and immunity in dogs

“…Lactobacillus plantarum strain RW1 was also reported to reduce the release of proinflammatory cytokines from Salmonella spp. [ 28 ]. Lactobacillus rhamnosus MP01 and MP02 increased fecal Lactobacillus counts in dogs and provided protection against gastrointestinal infection [ 29 ].…”

Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FRT7 from Chinese Paocai

“…Furthermore, Lp. plantarum strains have been used as probiotics due to their immunomodulation properties [ 31 ], pathogen suppression [ 32 ], modulation of intestinal balance [ 33 ], and cholesterol-lowering ability [ 34 ]. Therefore, GABA produced by Lp.…”