Is <i>Helicobacter pylori</i> a True Microaerophile?

Bury‐Moné, Stéphanie; Kaakoush, Nadeem O.; Asencio, Corinne; Mégraud, Françis; Thibonnier, Marie; Reuse, Hilde De; Mendz, George L.

doi:10.1111/j.1523-5378.2006.00413.x

Cited by 55 publications

(53 citation statements)

References 44 publications

(47 reference statements)

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“…In contrast to these findings, Xia et al claimed that most of their H. pylori strains grew under aerobic conditions but with reduced cell counts and smaller colonies (585). This discrepancy can be explained by the fact that, at low bacterial concentrations, H. pylori is microaerophilic, while at high bacterial concentrations, it can grow aerobically (50). Several systems can be used to achieve a microaerobic atmosphere, from the most sophisticated systems, such as a microaerobic cabinet or an incubator with an adjustable gas level, to jars in which the adequate atmosphere is created with an automatic apparatus (Anoxomat, MART Microbiology BV, Lichtenwoorde, The Netherlands) or with H 2 -CO 2 -generating packs.…”

Section: Vol 20 2007contrasting

confidence: 38%

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

2007

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SUMMARY The discovery of Helicobacter pylori in 1982 was the starting point of a revolution concerning the concepts and management of gastroduodenal diseases. It is now well accepted that the most common stomach disease, peptic ulcer disease, is an infectious disease, and all consensus conferences agree that the causative agent, H. pylori, must be treated with antibiotics. Furthermore, the concept emerged that this bacterium could be the trigger of various malignant diseases of the stomach, and it is now a model for chronic bacterial infections causing cancer. Most of the many different techniques involved in diagnosis of H. pylori infection are performed in clinical microbiology laboratories. The aim of this article is to review the current status of these methods and their application, highlighting the important progress which has been made in the past decade. Both invasive and noninvasive techniques will be reviewed.

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Section: Vol 20 2007contrasting

confidence: 38%

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

2007

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“…H pylori has been considered a microaerophile; however, recent reports refer that, at high cell densities, the microaerophilic nature might not hold true (29). Indeed, our results show that an atmospheric oxygen range of 3-10% did not affect significantly the growth in the liquid medium.…”

Section: Discussionsupporting

confidence: 36%

Optimization and Culture Conditions to Improve Helicobacter Pylori Growth in HAM's F-12 Medium by Response Surface Methodology

Bessa

Correia

Cellini

et al. 2012

Int J Immunopathol Pharmacol

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Helicobacter pylori is a gastroduodenal pathogen that colonizes the human stomach and is the causal agent of gastric diseases. From the clinical and epidemiological point of view, enhancing and improving the growth of this bacterium in liquid media is an important goal to achieve in order to allow the performance of accurate physiological studies. The aim of this work was to optimize three culture conditions that influence the growth of H. pylori in the defined medium Ham's F-12 supplemented with 5% fetal bovine serum by using response surface methodology as a statistical technique to obtain the optimal conditions. The factors studied in this experimental design (Box-Behnken design) were the pH of the medium, the shaking speed (rpm) and the percentage of atmospheric oxygen, in a total of 17 experiments. The biomass specific growth rate (J1) was the response measured. The model was validated for pH and shaking speed. The percentage of atmospheric oxygen did not influence the growth for the range of values studied. At the optimal values found for pH and shaking speed, 8 and 130 rpm, respectively, a specific growth rate value of 0.164 hot, corresponding to a maximal concentration of approximately 1.5xl0 8 CFU/ml, was reached after 8 h. The experimental design strategy allowed, for the first time, the optimization of H. pylori growth in a semi-synthetic medium, which may be important to improve physiological and metabolic studies of this "fastidious" bacterium.

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“…In order to establish an infection and to persist in the stomach, H. pylori must overcome the host innate immune response, including macrophages (1,9,32,41). For optimal in vitro growth, microaerophilic conditions (low levels of O 2 ) and capnophilic conditions (high levels of CO 2 ) are used and media are supplemented with serum (6,12,33). However, in vitro the viability of H. pylori is reduced to the noncultivatable level within 7 to 10 days.…”

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confidence: 99%

Regulation of Cell Growth during Serum Starvation and Bacterial Survival in Macrophages by the Bifunctional Enzyme SpoT in Helicobacter pylori

et al. 2008

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In Helicobacter pylori the stringent response is mediated solely by spoT. The spoT gene is known to encode (p)ppGpp synthetase activity and is required for H. pylori survival in the stationary phase. However, neither the hydrolase activity of the H. pylori SpoT protein nor the role of SpoT in the regulation of growth during serum starvation and intracellular survival of H. pylori in macrophages has been determined. In this study, we examined the effects of SpoT on these factors. Our results showed that the H. pylori spoT gene encodes a bifunctional enzyme with both a hydrolase activity and the previously described (p)ppGpp synthetase activity, as determined by introducing the gene into Escherichia coli relA and spoT defective strains. Also, we found that SpoT mediates a serum starvation response, which not only restricts the growth but also maintains the helical morphology of H. pylori. Strikingly, a spoT null mutant was able to grow to a higher density in serum-free medium than the wild-type strain, mimicking the "relaxed" growth phenotype of an E. coli relA mutant during amino acid starvation. Finally, SpoT was found to be important for intracellular survival in macrophages during phagocytosis. The unique role of (p)ppGpp in cell growth during serum starvation, in the stress response, and in the persistence of H. pylori is discussed.

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Is Helicobacter pylori a True Microaerophile?

Cited by 55 publications

References 44 publications

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

Optimization and Culture Conditions to Improve Helicobacter Pylori Growth in HAM's F-12 Medium by Response Surface Methodology

Regulation of Cell Growth during Serum Starvation and Bacterial Survival in Macrophages by the Bifunctional Enzyme SpoT in Helicobacter pylori

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