Is HPV virus associated with human breast cancer? Time to re-examine the postulate. Egyptian Study

Elsheshtawy, Nadia M.; Shakweer, Marwa M; Elghamrini, Yasser

doi:10.21276/apalm.2017.1143

Cited by 2 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sample size calculation: the total number of cases that attended NCI with BC was estimated to be 2500 per year. A previous study conducted in Egypt by El-Sheshtawy et al (2017) 31 indicated that the overall prevalence of HPV in malignant breast tissue was 16.7%. For a two-sided 95% confidence interval for a single proportion based on these criteria, the minimum required sample size to evaluate the practice was 54 cases with a 10% margin of error.…”

Section: Methodsmentioning

confidence: 93%

“… 39 Another report from Egypt demonstrated the presence of HPV DNA in 16.7% of fresh BC tissues. 31 Other studies demonstrated the absence of HPV DNA in fresh tissues. 40 The difference in result may be due to low viral load, 41 , 42 possible inter-laboratory inconsistency in collecting and handling samples 38 and storage of specimens.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Presence of HPV, EBV and HMTV Viruses Among Egyptian Breast Cancer Women: Molecular Detection and Clinical Relevance

Metwally¹,

Abo-Shadi²,

Fattah³

et al. 2021

IDR

View full text Add to dashboard Cite

Background Oncogenic viruses, their possible association with breast cancer (BC) and effect on its clinical course are interesting issue. The present study evaluates the presence of human papillomavirus (HPV), EpsteinBarr virus (EBV), and human mammary tumor virus (HMTV) in BC and their relation with clinico-pathological characteristics. Patients and Methods This study was conducted on 80 Egyptian women with BC and 30 control women without known oncological disease. Forty formalin-fixed paraffin-embedded (FFPE) tissues, forty fresh tissue samples, and white blood cells (WBCs) of BC patients and WBCs of controls were subjected to a qualitative polymerase chain reaction (PCR). Quantitative real-time PCR was used to measure viral loads in fresh tissues of BC. The result was correlated with clinico-pathological characteristics of BC. Results HPV was detected in 33 (41.25%), EBV in 30 (37.5%) and HMTV in 33 (41.25%) BC patients. None of the control women was positive for HPV or EBV while HMTV was detected in 7 (23.3%). Among 40 BC WBCs specimens, HPV/HMTV were found together in 25%, followed by EBV/HMTV in 2.5% and EBV/HPV in 2.5%. However, the three viruses (HPV/EBV/HMTV) were found together in only 5%. In the 40 fresh BC tissues, the three viruses were found together in 12 (30%), EBV/HMTV in 7 (17.5%), HPV/HMTV in 4 (10%), and HPV/EBV in 4 (10%). EBV, HMTV, or multiple viral infections were associated with younger age of BC women. HPV, EBV, and HMTV median loads in fresh tissues were 4.8×10 3 copies/μL, 6.3×10 3 copies/μL, and 97 copies/μL, respectively. Conclusion WBCs could be a more suitable specimen instead of fresh tissue for HMTV detection in BC patients to avoid invasive procedures. The presence of HPV, EBV, and HMTV together in Egyptian women with BC was significantly associated with younger age.

show abstract

Section: Methodsmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 98%

Presence of HPV, EBV and HMTV Viruses Among Egyptian Breast Cancer Women: Molecular Detection and Clinical Relevance

Metwally¹,

Abo-Shadi²,

Fattah³

et al. 2021

IDR

View full text Add to dashboard Cite

show abstract

“…12 The frequency of infection with HR-HPV genotypes in Egyptian women with BC was reported (22.2%) and is consistent with previously published worldwide data. 12,28,[55][56][57][58][59][60][61][62][63] The lower HR-HPV detection rate reported in some studies may be a result of difficulties in PCR detection due to low viral load in BC in different populations. [64][65][66] We detected HR-HPV in cancerous tissue or noncancerous safety margin adjacent to BC tissue as previously reported.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Human Papillomavirus Infection and Risk Factors in Egyptian Women With Breast Cancer

El-Sheikh

Mousa

Tawfeik

et al. 2021

Breast Cancer�(Auckl)

View full text Add to dashboard Cite

Numerous risk factors for breast cancer (BC) have been identified. High-risk human papilloma virus (HR-HPV) is the etiological agent of cervical cancer and in some cases of head and neck cancer, specifically oropharyngeal cancer, but the role of HR-HPV in evoking neoplasia in BC is still unclear. In this study, all women above the age of 18 visiting the oncology clinic at Al-Azhar university hospital and Ain Shams specialized hospital between the period of February 2017 and March 2018 were invited to participate. We determined the prevalence of HR-HPV genotypes 16, 18, and 31 in breast tissue samples from 72 women with treatment-naïve BC and 15 women with benign breast lesions (BBL) by quantitative real-time PCR (qRT-PCR) and primer sets targeting the E6 and E7 regions. High-risk human papilloma virus DNA was detected in 16 of 72 (22.2%) BC cases (viral load range = 0.3-237.8 copies/uL) and 0 of 15 women with BBL. High-risk human papilloma virus was detected in 14 of 16 (87.5%), 2 of 16 (12.5%), and 0 of 16 (0%) for genotypes 16, 18, and 31, respectively. Forty-three age-matched healthy Egyptian women were enrolled as controls for assessment of local risk factors that can be used to initiate a strategy of BC prevention in Egypt. Assessment of the risk factors demonstrated that low education level, passive smoking, lack of physical activity, family history of cancer, and use of oral contraception were significant risk factors for BC. In conclusion, our results lead us to postulate that HR-HPV infection may be implicated in the development of some types of BC in Egyptian women. In addition, identification of local risk factors can support practical prevention strategies for BC in Egypt.

show abstract

Is HPV virus associated with human breast cancer? Time to re-examine the postulate. Egyptian Study

Cited by 2 publications

References 20 publications

Presence of HPV, EBV and HMTV Viruses Among Egyptian Breast Cancer Women: Molecular Detection and Clinical Relevance

Presence of HPV, EBV and HMTV Viruses Among Egyptian Breast Cancer Women: Molecular Detection and Clinical Relevance

Assessment of Human Papillomavirus Infection and Risk Factors in Egyptian Women With Breast Cancer

Contact Info

Product

Resources

About