Is feminization in alcoholic men due in part to portal hypertension: A rat model

Quantitative Studies of Liver Atrophy After Portacaval Shunt in the Rat

“…This effect is supported by the resultant measured hormonal changes and the increased estradiol demonstrated in this study is comparable to similar findings in man [25][26][27][28] and the rat [29]. The accumulation of estradiol in liver disease is a consequence of reduced hepatic clearance of androgens and sex hormones [26] as well as an increase in the nonhepatic peripheral aromatisation conversion of weak androgen to estrogens [29]. These hormonal al- ternations are known to lead to DNA fragmentation and apoptosis [32].…”

Testicular immunohistochemical and Ultrastructural changes associated with chronic cholestasis in rats: Effect of ursodeoxycholic acid

“…Microscopic examination of the testis of BDL rats showed tubular distortion and atrophy, accompanied by thick irregular boundaries, vacuolation, disorganized epithelium, and exfoliated cells. Similar testicular pathological observations were reported after bile acid supplementation [24] and in other models of liver disease [5,6,25].…”

The use of quantitative scintigraphy in the measurement of portal-systemic shunting in rats

“…One animal was excluded from the analysis because part of the dose was lost by extravasation. The average of the ROI determinations by three independent observers yielded a rvalue of 0.99 (y=0.97±2.40; Sy.x=5.08) for %PSS when calculated by formula (1). Thus, the degree of PSS seen in vitro could be predicted from the averaged scintigraphic findings with an accuracy of approximately ±10%.…”

“…1,3 Briefly, in animals weighing 150-250 g the portal vein was surgically exposed under ether anesthesia. A single ligature of 4-0 silk was placed around the portal vein between Vol.…”

“…1 Previously described reference methods for measuring PSS in small laboratory animals utilizing injections of radioactive microspheres into the portal system2.3 require sacrifice of the animal for the quantitation of radioactivity in the dissected isolated organs, and do not permit serial studies. We therefore developed an alternative method for in vivo measurement of PSS in rats by quantitative planar scintigraphy after intraportal injection of 99 mTc- MAA shunt percentages obtained by injection of either MAA (10-90 ,um diameter) or 15 ,um polystyrene microspheres in order to relate our results to those of other investigators.…”