IS FÆCAL Α1-Antitrypsin EXCRETION a RELIABLE SCREENING TEST FOR PROTEIN-LOSING ENTEROPATHY?

Haeney, M R; Carter, R.A.; Fields, J.; Thompson, Rachel; Asquith, P.

doi:10.1016/s0140-6736(79)92387-0

Cited by 29 publications

(9 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hill et al [3] also reported no false positive or false negative results while using AATC to evaluate enteric protein loss in 15 children. On the other hand, in two other studies using higher AATC levels as the cut-off (30 and 45 ml/day), the sensitivity was reported to be 80–100% and the specificity was 50–58% [5,6]. The explanation of poor sensitivity of AATC in our study remains uncertain and can be related to technical problems in faecal sample collection and processing.…”

Section: Discussioncontrasting

confidence: 68%

“…There were significant variations in the reported sensitivity and specificity of the AATC in diagnosing PLE in the literature [3,4,5,6,7]. The discrepancy may partly be accounted for by the difference in the ‘normal’ AATC level in various studies.…”

Section: Discussionmentioning

confidence: 99%

“…α 1 -Antitrypsin is a broad-spectrum protease inhibitor that is synthesized in the liver and is resistant to proteolytic degradation within intestinal secretion and faeces. Enteric protein loss can be demonstrated by measuring the clearance of α 1 -antitrypsin from the plasma [3,4,5,6,7]. Technetium-99m ( 99m Tc)-labelled HSA scintigraphy is another widely used diagnostic tool for PLE since its introduction in 1986 [8].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of Performance of Measurement of Faecal α1-Antitrypsin Clearance and Technetium-99m Human Serum Albumin Scintigraphy in Protein-Losing Enteropathy

et al. 2011

View full text Add to dashboard Cite

Background and Aim: Our study aimed to compare the performance of faecal α₁-antitrypsin clearance (AATC) and radiolabelled human serum albumin (HSA) scintigraphy in protein-losing enteropathy (PLE). Methods: Patients studied by both AATC and technetium-99m (^99mTc)-labelled HSA scintigraphy were recruited and categorized into PLE and non-PLE groups based on clinical and laboratory findings. The performance of AATC and ^99mTc-labelled HSA scintigraphy was evaluated using clinical diagnosis of PLE as a gold standard. Results: 29 patients were recruited and 13 patients were considered to have definite PLE (PLE group). In the PLE group, all patients had a positive HSA scinigraphy and 10 (77%) had demonstrable positive tracing in the early phase. Conversely, only 6 of them (46%) had elevated AATC level (>13 m/day). Results of ^99mTc-labelled HSA scan (but not AATC) showed significant agreement with the clinical diagnosis (ĸ 0.35, p = 0.013). ^99mTc-labelled HSA scintigraphy carried higher sensitivity (100 vs. 46%) and negative predictive value (100 vs. 63%) compared to AATC in diagnosing PLE. The correlation between the results of these two investigations was only modest (ĸ 0.27, p = 0.04). The area under the receiver operating characteristic curve of AATC level showed no optimal diagnostic cut-off for PLE. Conclusion:^99mTc-labelled HSA scintigraphy was superior to AATC in diagnosing PLE.

show abstract

Section: Discussioncontrasting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Performance of Measurement of Faecal α1-Antitrypsin Clearance and Technetium-99m Human Serum Albumin Scintigraphy in Protein-Losing Enteropathy

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Assessment of protein loss can also be performed by measuring faecal a 1 -antitrypsin loss, which is considered to parallel the faecal excretion of albumin (15)(16)(17)(18)(19)(20). Alpha-1antitrypsin is a major protease inhibitor in human plasma.…”

Section: Alpha-1-antitrypsinmentioning

confidence: 99%

Faecal Parameters in the Assessment of Activity in Inflammatory Bowel Disease

Veer

Biemond²,

Verspaget³

et al. 1999

Scandinavian Journal of Gastroenterology

View full text Add to dashboard Cite

Van der Sluys Veer A, Biemond I, Verspaget HW, Lamers CBHW. Faecal parameters in the assessment of activity in inflammatory bowel disease. Scand J Gastroenterol 1999;34 Suppl 230:106-10.Background: Determination of inflammatory activity is helpful when assessing the efficacy of drugs in therapeutic trials and in facilitating management of individual patients with inflammatory bowel disease (IBD). Faecal parameters have been hypothesized to be more specific than non-faecal measurements in the assessment of intestinal inflammation. Methods: Review of the literature on faecal measurements in IBD. Results and conclusions: Leakage of various proteins and leukocyte products into the intestinal lumen can be assessed and quantified in stool specimens and serve as a measurement of inflammatory activity. Several of these faecal parameters are raised in patients with IBD. There is a considerable overlap between patients with active and those with inactive disease, however, and the correlation of the faecal parameters with disease activity indices is often low. The value of a 1 -antitrypsin measurement in faeces in the assessment of intestinal inflammation has been well established. Further studies in patients with IBD are needed to determine whether other faecal parameters, such as lactoferrin, tumour necrosis factor a, PMNelastase, lysozyme, leucocyte esterase, immunoglobulin A, among others, are more accurate or costeffective than measurement of a 1 -antitrypsin in the stools of such patients.ORIGINAL ARTICLE Scand J Gastroenterol 1999 (Suppl 230) Scand J Gastroenterol Downloaded from informahealthcare.com by Lakehead University on 11/03/14For personal use only. Scand J Gastroenterol 1999 (Suppl 230) 110A. van der Sluys Veer et al.Scand J Gastroenterol Downloaded from informahealthcare.com by Lakehead University on 11/03/14For personal use only.

show abstract

“…Seit etwa 1960 werden elektrophoretische und immunologische Methoden zur Plasmaproteinbestimmuhgim Stuhl eingesetzt (Übersicht 14). In den letzten Jahren wurde vor allem Alpha-1-Antitrypsin im 0.43 0.63 Stuhl und im Plasma quantitativ immunologisch zur Erfassung der eiweißverlierenden Gastroenteropathien bestimmt (2,4,7,9). in einer eigenen Untersuchungsreihe konnten wir zeigen, daß eine einmalige, zufällig entnommene Stuhlprobe fmicrorandom-sampling) sich vortrefflich zur qualitativen Plasmaproteinbestimmung im Stuhl eignet und unter vergleichbaren experimentellen Bedingungen den klassischen okkulten Blutnachw^is in der Sensibilität übertraf (11 Hierzu wird der betreffende Parameter aus einem Präzisionskontrollserum an 20 aufeinander folgenden Tagen (bei seltenen Bestimmungen an 5 aufeinander folgenden Tagen) bestimmt und aus den Einzelwer-: ten der Mittelwert = ( jq = Sumn me der Einzelwerte, n = Zahl der Bestimmungen) sowie die Standardabweichung Tritt einer der unter (a)-(d) genannten Fälle auf, sind die Meßergebnisse der Patientenproben der letzten Serie zu verwerfen und nach Eliminierung der Fehlerursache zu wiederholen.…”

Section: Diskussionunclassified

Ausbildung und Beruf

1982

LaboratoriumsMedizin

View full text Add to dashboard Cite

Es wird ein Probenbehälter beschrieben, mit dem unter hygienischen Bedingungen kleine und vergleichbare Mengen von Siuhlproben entnommen, versandt, extrahiert, zentrifugiert und zur immunchemischen Untersuchung verwendet werden können. Der Probenbehälter besteht aus einem Reagenzröhrchen, einem Schraubverschluß mit zentraler verschließbarer Bohrung, die über eine Verbindungshülle in einen Probenbecher mündet. Der Probenbecher ist nach oben mit einer Platte verschlossen, die nach Eingang in das Labor der Expression und Zerkleinerung des Stuhls dient. Der Stuhl wird mit physiologischem Puffer und 0,1% Na-azide 1:2 (Gewicht: Volumen) extrahiert und mit der doppelten und radialen Geldiffusion auf das Vorkommen von Plasmaproteinen untersucht. Schlüsselwörter: Plasmaproteine i. St. -immunologische Bestimmung -Stuhlextraktion -Stuhlteströhrchen Summary:A micro sampling collecting device for fecal material is described which permit s random sampling, transportation, extraction, centrifugation offeces under optimal hygienic conditions. The device consists of a polystyrene tube with a perforated cap connected by a perforated tube with the sample collector. The tube and the sample collector are separated by a polystyrene plate. This plate is used for the expulsion offeces into the tube and for homogenization. Thefeces is extracted with PBS -OJ N aacide 1:2 (wjv). The supernatant is tested for plasma proteins by double and radial gel diffusion using specific antisera.

show abstract

IS FÆCAL Α1-Antitrypsin EXCRETION a RELIABLE SCREENING TEST FOR PROTEIN-LOSING ENTEROPATHY?

Cited by 29 publications

References 2 publications

Evaluation of Performance of Measurement of Faecal α1-Antitrypsin Clearance and Technetium-99m Human Serum Albumin Scintigraphy in Protein-Losing Enteropathy

Evaluation of Performance of Measurement of Faecal α1-Antitrypsin Clearance and Technetium-99m Human Serum Albumin Scintigraphy in Protein-Losing Enteropathy

Faecal Parameters in the Assessment of Activity in Inflammatory Bowel Disease

Ausbildung und Beruf

Contact Info

Product

Resources

About