Is digestive cathepsin D the rule in decapod crustaceans?

Martinez-Alarcon, Diana; Saborowski, Reinhard; Rojo‐Arreola, Liliana; García‐Carreño, Fernando

doi:10.1016/j.cbpb.2017.09.006

Cited by 17 publications

(5 citation statements)

References 44 publications

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“…However, the role of this enzyme remains unclear, and it appears to be induced by physiological processes [ 36 ]. The colorimetric determination of pepsin-like in the current study was based on the hydrolysis of non-specific substrate, hemoglobin, so that the detection of activity is likely an expression of other acidic proteases, such as lysosomal cathepsin D, that are involved in the extracellular and intracellular digestion processes [ 39 ]. Enzyme inhibitors should be used to identify the type of enzymes that are in the extracts.…”

Section: Discussionmentioning

confidence: 99%

Optimal Dietary Protein Requirement for Juvenile Sesarmid Crab (Episesarma singaporense)

Sudtongkong

Thongprajukaew

Saekhow

2020

Animals

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The optimal dietary protein requirement for sesarmid crabs (Episesarma singaporense) was investigated. Juvenile E. singaporense, individually reared in plastic glasses containing 250 mL sea water, were fed five fish meal-soybean meal-microbound diets variously containing dietary protein levels of 30%, 35%, 40%, 45% and 50% for six weeks. A completely randomized design was used in the experiment, comprising five treatments with 60 crabs in each. At the end of the experiment, a significant improvement in survival was observed in all treatments relative to the diet containing 30% dietary protein (p < 0.05) while the growth performance parameters did not differ across the five dietary groups. A significantly higher protein efficiency ratio was observed in the E. singaporense crabs receiving 45% dietary protein relative to the remaining treatments. The specific activities of the digestive enzymes, pepsin-like, trypsin, amylase, and lipase, and the amylase to trypsin ratio fluctuated across the five treatments, but that of chymotrypsin remained consistent, suggesting different nutritional responses to the various dietary protein levels. The crabs receiving the 45% protein diet had significantly higher in essential amino acid (EAA) profiles followed by the 40% protein diet, although some EAA values were only moderate. The pattern for the non-EAA (NEAA) was reversed, and the ΣEAA/ΣNEAA ratio was higher in the crabs receiving the 45% protein diets relative to the other treatments. Based on our investigations, the optimal dietary protein requirement achieving desirable characteristics of juvenile E. singaporense crabs was 45%. This finding would be a useful guideline in preparing artificial diets for the mariculture of this species.

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Section: Discussionmentioning

confidence: 99%

Optimal Dietary Protein Requirement for Juvenile Sesarmid Crab (Episesarma singaporense)

Sudtongkong

Thongprajukaew

Saekhow

2020

Animals

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“…Digestive enzyme activity has been used as an important indicator for evaluating the digestive capacity and nutritional status of different animals including crustaceans [54,55]. In addition, an appreciation of the digestion process is vital in terms of understanding the feeding and nutritional ecology of animals [53].…”

Section: Discussionmentioning

confidence: 99%

Biological Impact of Photoperiod on Fairy Shrimp (Branchinecta orientalis): Life History and Biochemical Composition

Farhadi

Kangarloei

Ahmad

et al. 2021

Biology

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B. orientalis, fairy shrimp, is often among the most conspicuous invertebrates inhabiting temporary aquatic habitats with a typical variation in environmental conditions. Its life history characteristics and biochemical composition were studied under four different photoperiodic regimes (24L:0D, 0L:24D, 16L:8D, and 12L:12D). The significantly highest cumulative and initial hatching rates (48 h) were obtained at 24L:0D (p < 0.05). Cultivating the larvae under different photoperiods did not significantly affect specific growth rate (SGR) (p > 0.05). However, higher final total body length and daily growth rate were recorded under constant darkness. Higher lipid content was found at 24L:0D to the extent that it was more than two times higher than that at 16L:8D and 12L:12D (p < 0.05). There was also a remarkable increase in body crude protein content at 24L:0D (p < 0.05). Body fatty-acid profiles of the fairy shrimps were also affected by culture condition (p < 0.05). Extension of lighting period resulted in a subtle increase in body contents of arginine, lysine, histidine, isoleucine, leucine, valine, methionine, and phenylalanine, especially in the group kept under a 16L:8D regime. The highest and lowest digestive enzyme activity was observed at 0L:24D and 24L:0D, respectively (p < 0.05). In contrast, the highest and lowest soluble protein content was recorded at 24L:0D and 0L:24D, respectively (p < 0.05). Similarly, antioxidant status was significantly higher at 0L:24D (p < 0.05). In conclusion, a 16L:8D light–dark cycle might be an optimal condition in terms of growth performance and physio-biochemical characteristics. These findings could be helpful in optimizing the rearing conditions for upscaling B. orientalis production.

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“…In 2017, the presence of the activity and mRNA for cathepsin D was reported in six decapod infraorders (Martínez‐Alarcón et al . ). Cathepsin D activity was found in all infraorders and seven species.…”

Section: Types Of Digestive Peptidasesmentioning

confidence: 97%

The toolbox for protein digestion in decapod crustaceans: a review

Toro¹,

García‐Carreño²

2018

Reviews in Aquaculture

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Pancrustacea, including both crustaceans and insects, is the most abundant and diverse clade of Animalia and occupy all types of environments. They are studied because of their ecological, agricultural, health and economic issues, some being delicatessens. Crustaceans evolved during the Cambrian, thanks to adaptations, such as macrophagy, that allowed them to prey on diverse and energy-rich foods, which depended on complex digestive systems that drove evolution. As heterotrophs, crustaceans depend on their ability to obtain nutrients to fulfil their material, mostly carbon and energy requirements. Protein is the most important nutrient; up to 70% dry mass of a crustacean is protein. Hence, food protein is needed to obtain free amino acids to synthesize protein de novo (from scratch), because animals cannot synthesize the ten essential amino acids de novo. The ability to digest protein must have emerged early in evolution, as shown by the finding of trypsin coding genes in eubacterial genomes. Primitive organisms selectively fixed the ability to use foreign protein as a source of amino acids by synthesizing enzymes, now termed peptidases, which were capable of recognizing and hydrolysing peptide bonds in a specific region of a polypeptide chain, based on specificity for the side chains of amino acid residues in the carboxylic side of the site of cleavage. Here, we present a brief history on what and how digestive peptidases in crustaceans became known.

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Is digestive cathepsin D the rule in decapod crustaceans?

Cited by 17 publications

References 44 publications

Optimal Dietary Protein Requirement for Juvenile Sesarmid Crab (Episesarma singaporense)

Optimal Dietary Protein Requirement for Juvenile Sesarmid Crab (Episesarma singaporense)

Biological Impact of Photoperiod on Fairy Shrimp (Branchinecta orientalis): Life History and Biochemical Composition

The toolbox for protein digestion in decapod crustaceans: a review

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