Is abnormal sperm function an indicator among couples with recurrent pregnancy loss?

Saxena, Pikee; Misro, M. M.; Chaki, Sankar P.; Chopra, K.; Roy, Somnath; Nandan, Devki

doi:10.1016/j.fertnstert.2007.09.031

Cited by 39 publications

(28 citation statements)

References 16 publications

(13 reference statements)

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“…As a result, other relative factors should also be given a consideration. Currently, an increasing amount of studies have found an important role of sperm chromatin assessment employing different methods in RPL [17,19]. Talebi et al [7] used four different cytochemical tests including aniline blue (AB), chromomycin A3 (CMA3), toluidine blue (TB), acridine orange (AO), as well as nuclear chromatin stability assay, to analyze sperm.…”

Section: Discussionmentioning

confidence: 99%

The impact of semen quality, occupational exposure to environmental factors and lifestyle on recurrent pregnancy loss

Wang

Zhou

Zhang

et al. 2013

J Assist Reprod Genet

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Purpose This study aimed to investigate the effect of male semen quality, occupational exposure, and lifestyle on recurrent pregnancy loss (RPL). Methods Information on couples' occupational exposure and lifestyle was collected using a detailed questionnaire from 68 RPL couples and 63 randomly selected healthy controls. Semen parameters were estimated by computer-assisted sperm analysis, and sperm nuclear status was detected with aniline blue (AB) staining. Results Patients in the RPL group had significantly lower viability, normal morphology, and total progressive motility of sperm, and a higher mean percentage of AB staining positive sperm compared with those of controls (P <0.05). There were no differences in sperm concentration, and motility between the groups (P >0.05). Significant odds ratio (OR) was found when occupational exposure and unhealthy habits were superimposed (OR: 11.965, P =0.005). Conclusions In addition to standard female factors for evaluating the risk for RPL, the use of male factors should also be taken into consideration. We found that sperm quality, occupational exposure, and lifestyle are factors that affect RPL. Consequently, occupational exposure and lifestyle factors should constitute an important section of questionnaires given to patients, and these factors should be evaluated by a clinician or trained staff.

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Section: Discussionmentioning

confidence: 99%

The impact of semen quality, occupational exposure to environmental factors and lifestyle on recurrent pregnancy loss

Wang

Zhou

Zhang

et al. 2013

J Assist Reprod Genet

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“…Due to the tight packaging afforded by the protamines, any modification or absence of these proteins lead to an anomaly in the packaging process of sperm nucleus and influence sperm quality (morphology) and fertilizing capacity. Abnormalities such as loosely packaged chromatin and damaged DNA have already been observed in poor quality semen samples [10]. Pervious studies have shown that protamine deficiency measured by CMA3 staining independently effect fertilization [1,20].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion

Kazerooni

Asadi

Jadid

et al. 2009

J Assist Reprod Genet

103

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Objective To evaluate the sperm's chromatin quality in couples with spontaneous recurrent abortion. Methods Thirty couples with spontaneous recurrent abortion (case group) and 30 fertile couples (control group) referring to Zeinabieh Gynecology clinic of Shiraz were included. Semen samples were collected for each participant and were used for standard semen analysis and sperm nuclear maturity tests including Chromomycin A3 (CMA3), Aniline Blue (AB) staining and Acridine Orange (AO) test (by light microscopy).Result Patients in case group had significantly higher percentage of CMA3 (p < 0.001) and AB (p <0.001) positive spermatozoa compared to controls. However AO results did not differ significantly between groups (p= 0.656). Sperm morphology and progressive motility were negatively correlated with CMA3 (p=0.001 and p=0.043) and AB (p=0.015 and p=0.031) respectively. Conclusion Evaluation of the sperm's quality via CMA3 and AB staining could be considered as one of the complementary tests of semen analysis for assessment of male factor in couples with spontaneous recurrent abortion.

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“…Subnormal scores were observed in 14(15%) individuals of RPL group. Saxena et al, [29] reported mean less scores for all the three function tests in RPL group when compared to controls. The pilot study carried out by Chaithra et al, [30] reported significantly lower scores for semen profile and sperm function tests in the RPL group when compared to the control group.…”

Section: Discussionmentioning

confidence: 94%

“…The failure of sperm decondensation in the oocytes may be a consequence of a subtle sperm abnormality like structural or biochemical defects associated with chromatin packaging or organization during spermatogenesis [29]. Chromatin damage precedes the loss of fertilization potential and poor embryo quality, resulting in pregnancy loss.…”

Section: Discussionmentioning

confidence: 99%

Positive Association of Sperm Dysfunction in the Pathogenesis of Recurrent Pregnancy Loss

Kavitha

Malini

2014

JCDR

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Is abnormal sperm function an indicator among couples with recurrent pregnancy loss?

Cited by 39 publications

References 16 publications

The impact of semen quality, occupational exposure to environmental factors and lifestyle on recurrent pregnancy loss

The impact of semen quality, occupational exposure to environmental factors and lifestyle on recurrent pregnancy loss

Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion

Positive Association of Sperm Dysfunction in the Pathogenesis of Recurrent Pregnancy Loss

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