Search citation statements
Paper Sections
Citation Types
Year Published
Publication Types
Relationship
Authors
Journals
SynopsisThe nature of a specific association between acriflavine and cellular DNA was investigated by subjecting the DNA of intact cells to denaturation, ultraviolet radiation, and base analog substitution. The predictable modifications of DNA structure were then correlated with the quantitative alterations in dye adsorption by the cells. It was concluded that, under experimental conditions of limited dye availability, the bonding of dye was restricted to sites of highest bonding affinity. These were shown to be situated between adjacent thymine molecules on a single strand of DNA. The bmethyl groups of thymine may endow the bond with its specificity.The high bonding affinity between diaminoacridines and nucleic acids is demonstrable intracellularly as well as in purified reaction systems. 1-- 8 We have conducted studies of the bonding of acriflavine and the DNA of intact cells in the hope that, by keeping the ordered structure of the nucleoprotein-cation complex as intact as possible, additional information about this affinity might be gained. By the term "specific association" we refer to the selective and near-stoichiometric adsorption of acriflavine to cellular DNA as previously ascertained and r e p~r t e d .~. '~ The conditions for this were met by limiting amounts of dye in a standardized experimental model which restricts the association of dye and cellular anions to sites of highest bonding affinity.We are now reporting some changes in this bond produced by modifying cellular DNA with heat, ultraviolet radiation, and base analog substitution. We interpret the data as support for the concepts recently advanced by several a~t h o r s '~-'~ which, in their aggregate, point away from the complete intercalation model proposed by Lermanl8 and toward one in which diaminoacridines associate preferentially with adjacent thymine-containin.g base pairs in a strong bond which is disposed toward one of the DNA strands. Our data further suggest that the preferred binding site is situated between two adjacent thymine molecules whose 5-methyl groups endow the interaction with its specificity. 695
SynopsisThe nature of a specific association between acriflavine and cellular DNA was investigated by subjecting the DNA of intact cells to denaturation, ultraviolet radiation, and base analog substitution. The predictable modifications of DNA structure were then correlated with the quantitative alterations in dye adsorption by the cells. It was concluded that, under experimental conditions of limited dye availability, the bonding of dye was restricted to sites of highest bonding affinity. These were shown to be situated between adjacent thymine molecules on a single strand of DNA. The bmethyl groups of thymine may endow the bond with its specificity.The high bonding affinity between diaminoacridines and nucleic acids is demonstrable intracellularly as well as in purified reaction systems. 1-- 8 We have conducted studies of the bonding of acriflavine and the DNA of intact cells in the hope that, by keeping the ordered structure of the nucleoprotein-cation complex as intact as possible, additional information about this affinity might be gained. By the term "specific association" we refer to the selective and near-stoichiometric adsorption of acriflavine to cellular DNA as previously ascertained and r e p~r t e d .~. '~ The conditions for this were met by limiting amounts of dye in a standardized experimental model which restricts the association of dye and cellular anions to sites of highest bonding affinity.We are now reporting some changes in this bond produced by modifying cellular DNA with heat, ultraviolet radiation, and base analog substitution. We interpret the data as support for the concepts recently advanced by several a~t h o r s '~-'~ which, in their aggregate, point away from the complete intercalation model proposed by Lermanl8 and toward one in which diaminoacridines associate preferentially with adjacent thymine-containin.g base pairs in a strong bond which is disposed toward one of the DNA strands. Our data further suggest that the preferred binding site is situated between two adjacent thymine molecules whose 5-methyl groups endow the interaction with its specificity. 695
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.