Photochemistry of the Nucleic Acids

Smith, Kendric C.

doi:10.1016/b978-1-4832-2739-9.50015-6

Cited by 52 publications

(11 citation statements)

References 127 publications

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“…However, what we observed is similar t o the description of efficient DNA-protein cross-linking by oxygen-dependent photodynamic action (Smith and Hanawalt, 1969). BrdUrd substitution also enhances DNA-protein cross-linking at 254 nm U V irradiation (Smith, 1964a).…”

Section: Discussionmentioning

confidence: 99%

Hoechst 33258 Dye Generates Dna‐protein Cross‐links During Ultraviolet Light‐induced Photolysis of Bromodeoxyuridine in Replicated and Repaired Dna

Xi-Cang

Morgan

Cleaver

1986

Photochem & Photobiology

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Abstract— Substitution of bromodeoxyuridine for thymidine in the DNA of mammalian cells sensitizes them to a range of wavelengths of ultraviolet light. Cells are also sensitized to photochemical reactions involving dyes such as Hoechst 33258, which is used to produce differential staining of chromatids according to their bromodeoxyuridine content. Irradiation with 313 nm light of human and hamster cells containing bromodeoxyuridine in their DNA produced single‐strand breaks, detectable by alkaline elution, but no DNA‐protein cross‐links. Irradiation with 360 nm light in the presence of Hoechst 33258 produced extensive DNA‐protein cross‐linkage as well as single‐strand breaks. These cross‐links were observed in DNA containing bromodeoxyuridine incorporated by either semiconservative or repair replication, and may provide a method for identification of proteins in close proximity to replication forks or repair sites. When the protein was removed with proteinase K, bromodeoxyuridine in repair patches after irradiation by doses of ultraviolet (254 nm) light as low as 0.26 J/m2 could readily be detected. Hoechst 33258‐mediated photolysis, therefore, provides a sensitive new technique for measuring repair replication after ultraviolet light irradiation.

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Section: Discussionmentioning

confidence: 99%

Hoechst 33258 Dye Generates Dna‐protein Cross‐links During Ultraviolet Light‐induced Photolysis of Bromodeoxyuridine in Replicated and Repaired Dna

Xi-Cang

Morgan

Cleaver

1986

Photochem & Photobiology

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“…Increased sensitivity toward cross-linking of proteins to BU-DNA occurring in bacterial systems was shown by Smith (1964b), who used DNA extractability criteria to study cross-linking in E. coli B/R in which 70% of the thymine in the cellular DNA had been replaced by BU. UV-induced cross-linking of protein in mammalian cells, in which thymine had been substituted with BU, was studied by Smets and Cornelis (1971).…”

Section: Rna Virusesmentioning

confidence: 98%

Cross-Linking of Proteins to Nucleic Acids by Ultraviolet Light

Shetlar

1980

Photochemical and Photobiological Reviews

100

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“…We have also found it to be quite stable to a variety of conditions as shown in Table 3. Failure to isolate uracil dimer from irradiated polymers would therefore 720t seem to be due to its chemical lability (unless its presence in a polynucleotide chain confers lability) but is more probably related to the frequency of neighbouring uracil residues and to the inefficiency of dimer formation because of competing hydration reactions (27).…”

Section: Fig 4 the Absorption Spectra Of Irradiated And Non-irradiamentioning

confidence: 99%

Photochemical Reactions of Thymine, Uracil, Uridine, Cytosine and Bromouracil in Frozen Solution and in Dried Films*

Smith

1963

Photochem & Photobiology

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144

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Abstract— The photochemistry of uracil, uridine, cytosine, thymine and broinouracil has been investigated in frozen aqueous solution and in dried films. Essentially the same photoproducts were obtained in the two conditions; however, the yield of photoproducts was considerably greater in frozen solution. Uracil forms a dimer which can exist in two forms. Some kinetic data are presented for the interconversion of these two forms, The mixed dimer of thymine and uracil can also exist in two forms. Uridine forms only one acid stable photoproduct and does not appear to form mixed photoproducts under the conditions used. Two new photoproducts of thymine other than the dimer are described. Cytosine was at first considered to be completely inert hut using more sensitive detecting equipment it has recently been found to form uracil dinier as a result of dinierization and deamination. The most remarkable response was shown by bromouracil. When irradiated by itself it formed no photoproducts but when irradiated in the presence of uracil, uridine, cytosine or NaOH it formed many photoproducts. Most of these products were devoid of bromide, but two still contained bromine. One of these has been identified as the mixed dimer of uracil and bromouracil while the other has been tentatively identified as the dimer of bromouracil. Dimers of thymine or bromouracil were not formed by X‐rays.

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Photochemistry of the Nucleic Acids

Cited by 52 publications

References 127 publications

Hoechst 33258 Dye Generates Dna‐protein Cross‐links During Ultraviolet Light‐induced Photolysis of Bromodeoxyuridine in Replicated and Repaired Dna

Hoechst 33258 Dye Generates Dna‐protein Cross‐links During Ultraviolet Light‐induced Photolysis of Bromodeoxyuridine in Replicated and Repaired Dna

Cross-Linking of Proteins to Nucleic Acids by Ultraviolet Light

Photochemical Reactions of Thymine, Uracil, Uridine, Cytosine and Bromouracil in Frozen Solution and in Dried Films*

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