Irradiation by ultraviolet light-emitting diodes inactivates influenza a viruses by inhibiting replication and transcription of viral RNA in host cells

Nishisaka-Nonaka, Risa; Mawatari, Kazuaki; Yamamoto, Tomomi M.; Kojima, Mizuki; Shimohata, Takaaki; Uebanso, Takashi; Nakahashi, Mutsumi; Emoto, Takahiro; Akutagawa, Masatake; Kinouchi, Yohsuke; Wada, Takahiro; Okamoto, Masayuki; Ito, Hiroshi; Yoshida, Kenichi; Daidoji, Tomo; Nakaya, Takaaki; Takahashi, Akira

doi:10.1016/j.jphotobiol.2018.10.017

Cited by 56 publications

(75 citation statements)

References 34 publications

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“…From these reports, low WL UV-LEDs around 260-266 nm would exhibit the highest microbial reduction among UV-LEDs and LP-UV lamps. We previously reported that irradiation by UVA (365 nm), UVB (310 nm), and UVC (280 nm)-LEDs inhibited the infectious titer of both IAV H1N1 and H5N1 subtypes [20]. In our study, we found that a UVC-LED with a peak WL of 280 nm showed the highest inactivation effect on IAVs among the UV-LEDs tested.…”

Section: Introductionsupporting

confidence: 49%

“…The damage to viral RNA by UV irradiation was measured by reverse transcription (RT)-quantitative real-time polymerase chain reaction (qPCR) as previously reported [20,24,25]. To isolate viral RNA from the viral suspension with or without UV irradiation, a QIAamp Viral RNA Mini Kit (Qiagen, Redwood City, CA, USA) was used according to the manufacturer's instructions.…”

Section: Rna Extraction and Rt-qpcrmentioning

confidence: 99%

“…Virus pellets were resuspended in PBS and purified the viral RNA by a QIAamp Viral RNA Mini Kit (Qiagen). The purified viral RNA were checked by RT-PCR for segment 4 (supplementary Figure S1), as previously reported [20].…”

Section: Calculation Of Correlation Coefficients Between the Absorbanmentioning

confidence: 99%

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Irradiation by a Combination of Different Peak-Wavelength Ultraviolet-Light Emitting Diodes Enhances the Inactivation of Influenza A Viruses

Kojima

Mawatari

Emoto³

et al. 2020

Microorganisms

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Influenza A viruses (IAVs) pose a serious global threat to humans and their livestock. This study aimed to determine the ideal irradiation by ultraviolet-light emitting diodes (UV-LEDs) for IAV disinfection. We irradiated the IAV H1N1 subtype with 4.8 mJ/cm2 UV using eight UV-LEDs [peak wavelengths (WL) = 365, 310, 300, 290, 280, 270, and 260 nm)] or a mercury low pressure (LP)-UV lamp (Peak WL = 254 nm). Inactivation was evaluated by the infection ratio of Madin–Darby canine kidney (MDCK) cells or chicken embryonated eggs. Irradiation by the 260 nm UV-LED showed the highest inactivation among all treatments. Because the irradiation-induced inactivation effects strongly correlated with damage to viral RNA, we calculated the correlation coefficient (RAE) between the irradiant spectrum and absorption of viral RNA. The RAE scores strongly correlated with the inactivation by the UV-LEDs and LP-UV lamp. To increase the RAE score, we combined three different peak WL UV-LEDs (hybrid UV-LED). The hybrid UV-LED (RAE = 86.3) significantly inactivated both H1N1 and H6N2 subtypes to a greater extent than 260 nm (RAE = 68.6) or 270 nm (RAE = 42.2) UV-LEDs. The RAE score is an important factor for increasing the virucidal effects of UV-LED irradiation.

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Section: Introductionsupporting

confidence: 49%

Section: Rna Extraction and Rt-qpcrmentioning

confidence: 99%

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Irradiation by a Combination of Different Peak-Wavelength Ultraviolet-Light Emitting Diodes Enhances the Inactivation of Influenza A Viruses

Kojima

Mawatari

Emoto³

et al. 2020

Microorganisms

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“…A deep ultraviolet light-emitting diode (DUV-LED) instrument generating around 250-300 nm wavelength has been reported to effectively inactivate microorganisms, including bacteria, viruses and fungi [8][9][10][11], but effects on SARS-CoV-2 have not been reported. We evaluated the antiviral efficacy of irradiation by DUV-LED, generating a narrow-range wavelength (280 ± 5 nm, mean ± SD) (Nikkiso Co., Tokyo, Japan), which we tested against SARS-CoV-2.…”

Section: Lettermentioning

confidence: 99%

Rapid inactivation of SARS-CoV-2 with deep-UV LED irradiation

Inagaki

Saito

Sugiyama

et al. 2020

Emerging Microbes & Infections

252

219

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The spread of novel coronavirus disease 2019 (COVID-19) infections worldwide has raised concerns about the prevention and control of SARS-CoV-2. Devices that rapidly inactivate viruses can reduce the chance of infection through aerosols and contact transmission. This in vitro study demonstrated that irradiation with a deep ultraviolet light-emitting diode (DUV-LED) of 280 ± 5 nm wavelength rapidly inactivates SARS-CoV-2 obtained from a COVID-19 patient. Development of devices equipped with DUV-LED is expected to prevent virus invasion through the air and after touching contaminated objects.

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“…Inactivation of influenza A viruses (IAVs) via sterilization equipment with light-emitting diodes (LEDs) at peak wavelengths of 365 nm (UVA-LED), 310 nm (UVB-LED), and 280 nm (UVC-LED) was tested. These tests proved a decreased accumulation of intracellular total viral RNA in infected Madin-Darby canine kidney cells as well as a suppressed accumulation of intracellular mRNA (messenger RNA), vRNA (viral RNA), and cRNA (complementary RNA), as measured by strand-specific RT-PCR [21]. UVA light's side effects on blood coagulation has undergone substantial testing in rabbit ear bleeding time.…”

Section: Discussionmentioning

confidence: 97%

Novel method for reduction of virus load in blood plasma by sonication

Pförringer¹,

Braun²,

Mühlhofer³

et al. 2020

Eur J Med Res

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Background: Aim of the present study is the evaluation of ultrasound as a physical method for virus inactivation in human plasma products prior to transfusion. Our study is focused on achieving a high level of virus inactivation simultaneously leaving blood products unaltered, measured by the level of degradation of coagulation factors, especially in third world countries where virus contamination of blood products poses a major problem. Virus inactivation plays an important role, especially in the light of newly discovered or unknown viruses, which cannot be safely excluded via prior testing. Methods: Taking into account the necessary protection of the relevant coagulation activity for plasma, the basis for a sterile virus inactivation under shielding gas insufflation was developed for future practical use. Influence of frequency and power density in the range of soft and hard cavitation on the inactivation of transfusion-relevant model viruses for Hepatitis-(BVDV = bovine diarrhea virus), for Herpes-(SFV = Semliki Forest virus, PRV = pseudorabies virus) and Parvovirus B19 (PPV = porcine parvovirus) were examined. Coagulation activity was examined via standard time parameters to minimize reduction of functionality of coagulation proteins. A fragmentation of coagulation proteins via ultrasound was ruled out via gel electrophoresis. The resulting virus titer was examined using end point titration. Results: Through CO 2 shielding gas insufflation-to avoid radical emergence effects-the coagulation activity was less affected and the time window for virus inactivation substantially widened. In case of the non-lipidated model virus (AdV-luc = luciferase expressing adenoviral vector), the complete destruction of the virus capsid through hard cavitation was proven via scanning electron microscopy (SEM). This can be traced back to microjets and shockwaves occurring in hard cavitation. The degree of inactivation seems to depend on size and compactness of the type of viruses. Using our pre-tested and subsequently chosen process parameters with the exception of the small PPV, all model viruses were successfully inactivated and reduced by up to log 3 factor. For a broad clinical usage, protection of the coagulation activities may require further optimization. Conclusions: Building upon the information gained, an optimum inactivation can be reached via raising of power density up to 1200 W and simultaneous lowering of frequency down to 27 kHz. In addition, the combination of the two physical methods UV treatment and ultrasound may yield optimum results without the need of substance removal after the procedure.

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Irradiation by ultraviolet light-emitting diodes inactivates influenza a viruses by inhibiting replication and transcription of viral RNA in host cells

Cited by 56 publications

References 34 publications

Irradiation by a Combination of Different Peak-Wavelength Ultraviolet-Light Emitting Diodes Enhances the Inactivation of Influenza A Viruses

Irradiation by a Combination of Different Peak-Wavelength Ultraviolet-Light Emitting Diodes Enhances the Inactivation of Influenza A Viruses

Rapid inactivation of SARS-CoV-2 with deep-UV LED irradiation

Novel method for reduction of virus load in blood plasma by sonication

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