A pot experiment was carried out to investigate the effect of iron supplementation on physiological and biochemical status of the low-chill peach cultivars (Saharanpur Prabhat, Shan-e-Punjab and Pratap) suffered from iron chlorosis in artificially created calcareous soil. Three most commonly used iron sources viz. Fe-sulphate (1.0 % and 0.5 %), Fe-citrate (1.0 % and 0.5 %) and FeEDTA (0.1 % and 0.2 %) were sprayed on the 4th and 5th leaves from the apex of the twig. And after 1 week of spraying, observation on various physiological and biochemical parameters in leaves were recorded. Improvement in plant physiological parameters like chlorophyll content index (CCI), photosynthetic rate (P n ), stomatal conductance (g s ) and intercellular CO 2 conc. (C i ) were recorded best with the application of 1.0 % Fe-sulphate both in treated and untreated upper leaves. The maximum recovery in biochemical parameters such as total leaf chlorophyll content, superoxide dismutase (SOD) and peroxidase (POD) activity was also noted with the application of 1.0 % Fe-sulphate. However, application of 1.0 % Fe-sulphate and 0.5 % Fesulphate had similar effect for most of the parameters under study. The ability of iron sources to induce physiological and biochemical responses in iron deficient low-chill peach plants were in the following order Fe-sulphate>Fe-citrate>FeEDTA. Differential responses in plant physiological and biochemical parameters were also exhibited by the low-chill peach cultivars with regard to supplementation of various iron sources. Among the low-chill peach cultivars, Saharanpur Prabhat responded best with the application of iron sources followed by Shan-e-Punjab and Pratap.