Iron regulates the expression of ferroportin 1 in the cultured hFOB 1.19 osteoblast cell line

Zhao, Guoyang; Di, Donghua; Wang, Bo; Zhang, Peng; Xu, Youjia

doi:10.3892/etm.2014.1823

Cited by 11 publications

(6 citation statements)

References 32 publications

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“…It is this context that we developed a FCM based method for detecting chelatable iron in M. gryphiswaldense MSR-1 cells using phen green™ SK (PG-SK), whose fluorescence is quenched by metal ions including Fe 2+ and Fe 3+ . PG-SK has been previously used to study iron transporters 54 and efflux systems in E. coli as well as applied to studies in human cell lines for iron, copper and silver uptake 55 , 56 .…”

Section: Resultsmentioning

confidence: 99%

Flow cytometry as a rapid analytical tool to determine physiological responses to changing O2 and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1

Fernández-Castañé

Liu

Thomas

et al. 2017

Sci Rep

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Magnetotactic bacteria (MTB) are a diverse group of bacteria that synthesise magnetosomes, magnetic membrane-bound nanoparticles that have a variety of diagnostic, clinical and biotechnological applications. We present the development of rapid methods using flow cytometry to characterize several aspects of the physiology of the commonly-used MTB Magnetospirillum gryphiswaldense MSR-1. Flow cytometry is an optical technique that rapidly measures characteristics of individual bacteria within a culture, thereby allowing determination of population heterogeneity and also permitting direct analysis of bacteria. Scatter measurements were used to measure and compare bacterial size, shape and morphology. Membrane permeability and polarization were measured using the dyes propidium iodide and bis-(1,3-dibutylbarbituric acid) trimethine oxonol to determine the viability and ‘health’ of bacteria. Dyes were also used to determine changes in concentration of intracellular free iron and polyhydroxylakanoate (PHA), a bacterial energy storage polymer. These tools were then used to characterize the responses of MTB to different O2 concentrations and iron-sufficient or iron-limited growth. Rapid analysis of MTB physiology will allow development of bioprocesses for the production of magnetosomes, and will increase understanding of this fascinating and useful group of bacteria.

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Section: Resultsmentioning

confidence: 99%

Flow cytometry as a rapid analytical tool to determine physiological responses to changing O2 and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1

Fernández-Castañé

Liu

Thomas

et al. 2017

Sci Rep

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show abstract

“…Excess iron facilitates osteoclast differentiation, which is multinucleated giant cells, and bone resorption [ 26 , 27 ]. Previous studies also have demonstrated that higher iron level will also increase the intracellular ferroportin 1 (FPN-1) in osteoblast and other cells to maintain the iron homeostasis [ 28 ]. Iron which is not utilized is stored as ferritin in some cell types.…”

Section: Resultsmentioning

confidence: 99%

A Wearable Iron-Based Implant as an Intramedullary Nail in Tibial Shaft Fracture of Sheep

Siallagan

Silalahi

Boediono

et al. 2019

International Journal of Biomaterials

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A stable repaired fracture is the key factor responsible for the recovery of a damaged bone. The iron-based implant is one of the biodegradable metals that have been proven safe as a fracture fixation device. The objective of our experimental approach was to examine the potential of the iron-based implant as a biodegradable metal in tibia shaft fracture in sheep chronically. The samples used for this experiment were iron-based and stainless steel implants. Each had a diameter of 5 mm. These samples were analyzed through 3 phases which are material characterization, in vitro and in vivo examination. The samples were examined using a scanning electron microscope with energy dispersive spectrometer and X-ray diffraction. Based on the analysis carried out, the samples contained 90,02% and 60,81% Fe for iron-based implant and stainless implant, respectively. Both implants maintained high viability when being in contact with calf pulmonary artery endothelial cells, indicating that both implants had a minimum response to the cell in a hemocytometer and methyl tetrazolium (MTT) assay. The systemic effect of the implants was observed using hematology and blood chemistry examination. Data collection also shows that both implants also had a minimum response to the erythrocytes, leucocytes, blood chemistry, and blood mineral during the period of observation. Therefore, it could be concluded that the iron-based implant is tolerable for a period of 9 months. It also has the potential to be used as a biodegradable orthopedic implant.

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“…Indeed, HO-1 knockout in mice and HO-1 deficiency in humans is linked to anemia, due to accumulation of iron in tissues and decreased iron recycling 18 , 20 , 65 , whereas overexpression of HO-1 is associated with increased cellular iron efflux and decreased influx 66 . FAC has also been reported to increase ferritin synthesis 67 and ferroportin expression 68 , 69 . Taken together, these data suggest that an increase in HO-1 expression/activity would be expected to result in a secondary decrease in labile or “chelatable” iron, which has implications for downstream signaling.…”

Section: Discussionmentioning

confidence: 97%

Fluid shear stress regulates placental growth factor expression via heme oxygenase 1 and iron

Rashdan

Zhai

Lovern

2021

Sci Rep

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Increased fluid shear stress (FSS) is a key initiating stimulus for arteriogenesis, the outward remodeling of collateral arterioles in response to upstream occlusion. Placental growth factor (PLGF) is an important arteriogenic mediator. We previously showed that elevated FSS increases PLGF in a reactive oxygen species (ROS)-dependent fashion both in vitro and ex vivo. Heme oxygenase 1 (HO-1) is a cytoprotective enzyme that is upregulated by stress and has arteriogenic effects. In the current study, we used isolated murine mesentery arterioles and co-cultures of human coronary artery endothelial cells (EC) and smooth muscle cells (SMC) to test the hypothesis that HO-1 mediates the effects of FSS on PLGF. HO-1 mRNA was increased by conditions of increased flow and shear stress in both co-cultures and vessels. Both inhibition of HO-1 with zinc protoporphyrin and HO-1 knockdown abolished the effect of FSS on PLGF. Conversely, induction of HO-1 activity increased PLGF. To determine which HO-1 product upregulates PLGF, co-cultures were treated with a CO donor (CORM-A1), biliverdin, ferric ammonium citrate (FAC), or iron-nitrilotriacetic acid (iron-NTA). Of these FAC and iron-NTA induced an increase PLGF expression. This study demonstrates that FSS acts through iron to induce pro-arteriogenic PLGF, suggesting iron supplementation as a novel potential treatment for revascularization.

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Iron regulates the expression of ferroportin 1 in the cultured hFOB 1.19 osteoblast cell line

Cited by 11 publications

References 32 publications

Flow cytometry as a rapid analytical tool to determine physiological responses to changing O2 and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1

Flow cytometry as a rapid analytical tool to determine physiological responses to changing O2 and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1

A Wearable Iron-Based Implant as an Intramedullary Nail in Tibial Shaft Fracture of Sheep

Fluid shear stress regulates placental growth factor expression via heme oxygenase 1 and iron

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