Iron homeostasis and eye disease

Loh, Allison R.; Hadziahmetovic, Majda; Dunaief, Joshua L.

doi:10.1016/j.bbagen.2008.11.001

Cited by 108 publications

(91 citation statements)

References 129 publications

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“…Iron is crucial for cell survival, and stable transferrin expression has been demonstrated to play a major role in iron homeostasis within the retina (43). Iron storage is regulated by ferritin and iron export by proteins such as ceruloplasmin, ferroportin, and hephaestin, and transferrin is involved in iron import into the cell (44).…”

Section: Discussionmentioning

confidence: 99%

Identification of a Novel Neurotrophic Factor from Primary Retinal Müller Cells Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)

Toerne

Menzler

et al. 2014

Molecular & Cellular Proteomics

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Retinal Mü ller glial cells (RMGs) have a primary role in maintaining the homeostasis of the retina. In pathological situations, RMGs execute protective and regenerative effects, but they can also contribute to neurodegeneration. It has recently been recognized that cultured primary RMGs secrete pro-survival factors for retinal neurons for up to 2 weeks in culture, but this ability is lost when RMGs are cultivated for longer durations. In our study, we investigated RMG supernatants for novel neuroprotective factors using a quantitative proteomic approach. Stable isotope labeling by amino acids in cell culture (SILAC) was used on primary porcine RMGs. Supernatants of RMGs cultivated for 2 weeks were compared with supernatants from cells that had already lost their protective capacity. Using this approach, we detected established neurotrophic factors such as transferrin, osteopontin, and leukemia inhibitory factor and identified C-X-C motif chemokine 10 (CXCL10) as a novel candidate neuroprotective factor. All factors prolonged photoreceptor survival in vitro. Ex vivo treatment of retinal explants with leukemia inhibitory factor or CXCL10 demonstrated a neuroprotective effect on photoreceptors. Western blots on CXCL10-and leukemia inhibitory factor-stimulated explanted retina and photoreceptor lysates indicated activation of pro-survival signal transducer and activator of transcription signaling and B-cell lymphoma pathways. These findings suggest that CXCL10 contributes to the supportive potential of RMGs toward retinal neurons. Molecular & Cellular Proteomics 13:

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Section: Discussionmentioning

confidence: 99%

Identification of a Novel Neurotrophic Factor from Primary Retinal Müller Cells Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)

Toerne

Menzler

et al. 2014

Molecular & Cellular Proteomics

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“…The latter has been correlated with DNA oxidation and down-regulation of p53 (40). Interestingly, it has been suggested that the presence of iron deposits along the corneal head of pterygium may indicate oxidative stress affecting local epithelial cells and resulting in disturbed iron homeostasis (35). The topical use of antioxidants as an adjuvant treatment option for pterygium would thus seem logical and should be investigated in future research projects.…”

Section: Concepts Of Inheritancementioning

confidence: 99%

“…The presence of Stocker's line along the head of pterygia lacking growth potential, as previously mentioned, may represent abnormal local iron metabolism along the advancing head of pterygium. Iron itself may be associated with increased free radical formation through biochemical (Fenton) reactions (35).…”

Section: Concepts Of Inheritancementioning

confidence: 99%

Pathogenetic mechanisms and treatment options for ophthalmic pterygium: Trends and perspectives (Review)

Detorakis

Spandidos²

2009

Int J Mol Med

150

103

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Abstract. Pterygium is a fibrovascular lesion of the ocular surface that can display an aggressive clinical behavior and, occasionally, threaten vision. Although there is no consensus on its pathogenesis, recent evidence suggests that it is a proliferative, rather than degenerative condition strongly correlated with exposure to ultraviolet radiation (UVR) of solar light. Molecular genetic alterations reported in association with pterygium include loss of heterozygosity (LOH), point mutations of proto-oncogenes, such as K-ras and alterations in the expression of tumor suppressor genes, such as p53 or p63. Other findings in pterygium include the frequent detection of HPV DNA, ocular surface changes such as the overexpression of various proteins, including defensins and phospolipases D, as well as the up-regulation of growth factors, such as bFGF or VEGF. Although pterygium management has traditionally involved surgery, often enhanced by the use of antimetabolites, recent advancements in the understanding of molecular and biochemical events underlying pterygium pathogenesis may enable the use of less invasive treatment methods.

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“…При некоторых заболеваниях глаз выявляют на-рушение минерального обмена в тканях, отводя особую роль Fe [22,23]. Отложение пигмента неред-ко происходит именно в роговице.…”

Section: Sem-edsunclassified