Iron biofortification and availability in the mycelial biomass of edible and medicinal basidiomycetes cultivated in sugarcane molasses

Scheid, Simone Schenkel; Faria, Maria Graciela Iecher; Velasquez, Leonardo Garcia; Valle, Juliana Silveira do; Gonçalves, Affonso Celso; Dragunski, Douglas Cardoso; Colauto, Nelson Barros; Linde, Giani Andréa

doi:10.1038/s41598-020-69699-0

Cited by 17 publications

(6 citation statements)

References 17 publications

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“…The differences in Mg concentration probably influenced these observations. Interestingly, studies of the effect of the addition of Mn to Fe-fortified substrate on the growth and accumulation of L. crinitus have been published by Meniqueti et al [40]. The authors tested the effect of Mn (0.9 mg L −1 ) on mushroom growth, bioaccumulation, and transfer of Fe at different cultivation times (7, 14, and 21 days) in a culture medium.…”

Section: Discussionmentioning

confidence: 99%

Influence of Iron Addition (Alone or with Calcium) to Elements Biofortification and Antioxidants in Pholiota nameko

Budzyńska

Siwulski

Magdziak

et al. 2021

Plants

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Mushrooms supplementation with iron (Fe) is usually limited, and therefore it would be beneficial to search for other vital elements able to improve the process. The aim of this study was to verify a possible interaction between Fe and calcium (Ca) to estimate the role of the addition of the latter metal to stimulate Fe accumulation in Pholiota nameko. Additionally, an analysis of phenolic compounds and low molecular weight organic acids (LMWOAs) was performed. The increase of Fe concentration in the substrate caused a significantly higher accumulation of this metal in P. nameko. The addition of Ca (5 or 10 mM) stimulated Fe accumulation, just as Fe concentration in the substrate stimulated Ca accumulation, which pointed to a synergism between these metals. The obtained results show that the presence of Fe in the substrate may also promote K, Mg, Mn, Na, P, and S accumulation. In contrast, the addition of Ca stimulates and/or inhibits their content in fruit bodies. The phenolic and organic acids profile was poor. Only gallic, 4-hydroxybenzoic, sinapic and syringic acids (phenolics), as well as citric and succinic acids (LMWOAs), were quantified in some combinations in P. nameko fruiting bodies.

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Section: Discussionmentioning

confidence: 99%

Influence of Iron Addition (Alone or with Calcium) to Elements Biofortification and Antioxidants in Pholiota nameko

Budzyńska

Siwulski

Magdziak

et al. 2021

Plants

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“…Biological efficiency, nutrient contents, and antioxidant activity in this mushroom species when 500 mg kg −1 Fe was added to a substrate-containing rice bran and maize stalk were described. Iron biofortification and availability from L. crinitus , G. lucidum , Schizophyllum commune , P. ostreatus , P. eryngii , L. edodes, and A. subrufescens grown in two different culture media (malt extract with 0.116 mg L −1 or sugarcane molasses with 91.2 mg L −1 ) were described by Scheid et al [ 56 ]. A comparison of Fe bioaccumulation in mycelial biomass of Letinula edodes , P. eryngii , P. ostreatus, and S. commune grown in malt extract agar with 50 mg L −1 enrichment was made by Umeo et al [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Biofortification of Three Cultivated Mushroom Species with Three Iron Salts—Potential for a New Iron-Rich Superfood

et al. 2022

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Mushrooms fortified with iron (Fe) can offer a promising alternative to counter the worldwide deficiency problem. However, the factors that may influence the efficiency of fortification have not yet been fully investigated. The aim of this study was to compare the effects of three Fe forms (FeCl3 6H2O, FeSO4 7H2O, or FeHBED) in three concentrations (5, 10, or 50 mM) for three mushroom species (Pleurotus eryngii, P. ostreatus, or Pholiota nameko) on their chemical composition, phenolic compounds, and organic acid production. The most effective metal accumulation of all the investigated species was for the 50 mM addition. FeCl3 6H2O was the most favorable additive for P. eryngii and P. nameko (up to 145 and 185% Fe more than in the control, respectively) and FeHBED for P. ostreatus (up to 108% Fe more than in control). Additionally, P. nameko showed the highest Fe accumulation among studied species (89.2 ± 7.51 mg kg−1 DW). The creation of phenolic acids was generally inhibited by Fe salt supplementation. However, an increasing effect on phenolic acid concentration was observed for P. ostreatus cultivated at 5 mM FeCl3 6H2O and for P. eryngii cultivated at 5 mM FeCl3 6H2O and 5 mM FeSO4 7H2O. In the case of organic acids, a similar situation was observed. For P. ostreatus, FeSO4 7H2O and FeHBED salts increased the formation of the determined organic acids in fruiting bodies. P. eryngii and P. nameko were characterized by a much lower content of organic acids in the systems supplemented with Fe. Based on the obtained results, we recommend starting fortification by preliminarily indicating which form of the element is preferred for the species of interest for supplementation. It also seems that using an additive concentration of 50 mM or higher is most effective.

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“…Ganoderma lucidum (Curtis) P. Karst. (Basidiomycotaete: Polyporales) has the ability to bioaccumulate metals of nutritional and pharmacological interest in its mycelial structures (MENIQUETI et al, 2020;SCHEID et al, 2020;UMEO et al, 2020;MATUTE et al, 2011;MENIQUETI et al, 2021). The bioaccumulation of metals in basidiomycetes is a particularly interesting strategy to control the solubility and availability of metals.…”

Section: Introductionmentioning

confidence: 99%

Acute safety assessment of lithium bioacummulated in mycelial biomass of Ganoderma lucidum

Silva¹,

Amaral²,

Silva³

et al. 2022

CONJ

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Lithium is a first-line treatment for bipolar disorder, a disorder that is characterized by alternating manic and depressive episodes. However, because of its narrow therapeutic index, lithium can cause adverse effects. The association of lithium with more complex molecules, such as proteins and polysaccharides, may help control the release of lithium throughout the digestive system, thereby reducing the doses that are used by patients and consequently reducing adverse effects. The present study evaluated the acute oral toxicity of lithium bioaccumulated in mycelial biomass of Ganoderma lucidum, a research step that precedes preclinical efficacy studies. The study followed Organization for Economic Cooperation and Development guideline. Male Wistar rats (n = 6) were acutely treated by gavage with fixed doses (300 and 2000 mg/kg) of lithium bioaccumulated in mycelial biomass of G. lucidum. Changes in clinical signs, body mass, relative organ mass, biochemical parameters, blood count, leukocytes, and histopathological changes were evaluated. A group of animals that were treated with vehicle (n = 6) served as the basal group. No signs of toxicity were observed in any of the investigated parameters. Lithium bioaccumulation in mycelial biomass of G. lucidum did not induce signs of toxicity in rats that were acutely orally treated.

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Iron biofortification and availability in the mycelial biomass of edible and medicinal basidiomycetes cultivated in sugarcane molasses

Cited by 17 publications

References 17 publications

Influence of Iron Addition (Alone or with Calcium) to Elements Biofortification and Antioxidants in Pholiota nameko

Influence of Iron Addition (Alone or with Calcium) to Elements Biofortification and Antioxidants in Pholiota nameko

Biofortification of Three Cultivated Mushroom Species with Three Iron Salts—Potential for a New Iron-Rich Superfood

Acute safety assessment of lithium bioacummulated in mycelial biomass of Ganoderma lucidum

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